Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,531,596 (G)6→5 coding (134/762 nt) cysZ → sulfate transporter, sulfite inhibited

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,531,5910G.99.1% 498.0 / ‑3.0 112coding (129/762 nt)cysZsulfate transporter, sulfite inhibited
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base . (53/57);  minor base T (1/0);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCT  >  NC_000913/2531494‑2531686
                                                                                                 |                                                                                               
gCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATggg                                                                                               >  1:1605496/1‑100 (MQ=255)
 cGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgg                                                                                                <  1:1420746/98‑1 (MQ=255)
  ggTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATggggg                                                                                             >  1:2672917/1‑100 (MQ=255)
    tttttACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgggggcg                                                                                           <  2:1551812/100‑3 (MQ=255)
      tttACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgggggcgca                                                                                         <  2:441679/100‑5 (MQ=255)
       ttACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCAt                                                                                       >  2:1057342/1‑100 (MQ=255)
          cTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGTGGCGCATTCt                                                                                    >  1:2230233/1‑100 (MQ=255)
          cTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCt                                                                                    <  1:717869/100‑1 (MQ=255)
            aTTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtgg                                                                                  <  1:2120463/100‑1 (MQ=255)
            aTTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtgg                                                                                  >  2:1044467/1‑100 (MQ=255)
            aTTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtgg                                                                                  <  2:1944417/100‑1 (MQ=255)
            aTTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtgg                                                                                  <  1:193830/100‑1 (MQ=255)
             ttttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtggt                                                                                 >  2:961201/1‑100 (MQ=255)
              tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtggtg                                                                                <  2:1935010/100‑1 (MQ=255)
              tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtggtg                                                                                <  2:680159/100‑1 (MQ=255)
              tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTGGGTTATTTTACCGCTGCTGGTCAATATGTTGTTGGTGGGGGCGCATGgtggtg                                                                                >  2:1992305/1‑100 (MQ=255)
               ttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtggtgg                                                                               <  1:1993721/100‑1 (MQ=255)
                tGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGc                                                                              <  1:2544596/100‑1 (MQ=255)
                  cgcAAGGCTGGAAGCTCGTCACGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGctc                                                                            >  1:1780301/1‑100 (MQ=255)
                    cAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCtt                                                                          <  2:1931100/100‑1 (MQ=255)
                    cAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCtt                                                                          >  1:230736/1‑100 (MQ=255)
                    cAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGTTGGGGGCGCATTCTGGTGGCTCtt                                                                          >  1:2456070/1‑100 (MQ=255)
                      aGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTa                                                                        <  1:1058109/100‑1 (MQ=255)
                      aGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTa                                                                        <  2:1115103/100‑1 (MQ=255)
                      aGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTa                                                                        <  2:77129/100‑1 (MQ=255)
                       ggCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTa                                                                        >  2:1743533/1‑100 (MQ=255)
                       ggCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCtttcc                                                                       <  2:1960350/100‑3 (MQ=255)
                       ggCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTac                                                                       >  2:319831/1‑100 (MQ=255)
                       ggCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTac                                                                       <  1:2445241/100‑1 (MQ=255)
                        gCTGGAAGCTCGTTTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTaca                                                                      >  2:1970189/1‑100 (MQ=255)
                        gCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTaca                                                                      >  2:1529967/1‑100 (MQ=255)
                         cTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTacac                                                                     <  2:2694626/100‑1 (MQ=255)
                          tGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTAcaca                                                                    <  1:1970161/100‑1 (MQ=255)
                          tGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTAcaca                                                                    >  2:957536/1‑100 (MQ=255)
                            gAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGc                                                                  >  2:2148350/1‑100 (MQ=255)
                              aGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTc                                                                <  2:1859158/100‑1 (MQ=255)
                              aGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTc                                                                >  2:2642320/1‑100 (MQ=255)
                                cTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGa                                                              >  1:2204193/1‑100 (MQ=255)
                                  cGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATg                                                            >  2:901107/1‑100 (MQ=255)
                                  cGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATg                                                            >  1:927419/1‑100 (MQ=255)
                                    tctcGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTc                                                          <  2:1322136/100‑1 (MQ=255)
                                     ctcGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCt                                                         >  2:1356920/1‑100 (MQ=255)
                                      tcGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTg                                                        <  1:973727/100‑1 (MQ=255)
                                      tcGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTg                                                        <  1:216546/100‑1 (MQ=255)
                                       cGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTgg                                                       >  1:804015/1‑100 (MQ=255)
                                       cGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTgg                                                       >  1:1659282/1‑100 (MQ=255)
                                        gCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGa                                                      >  2:1176392/1‑100 (MQ=255)
                                        gCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGa                                                      <  1:453562/100‑1 (MQ=255)
                                           aCCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTTGCTCTTTACACAGCTCGATGTCTGGATcc                                                   <  1:723291/100‑1 (MQ=255)
                                           aCCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATcc                                                   >  2:290477/1‑100 (MQ=255)
                                           aCCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATcc                                                   >  1:2063775/1‑100 (MQ=255)
                                            ccTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATccc                                                  <  1:1055202/100‑1 (MQ=255)
                                             cTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCg                                                 >  1:1632390/1‑100 (MQ=255)
                                              tGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGa                                                >  2:953753/1‑100 (MQ=255)
                                              tGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGa                                                >  2:2064787/1‑100 (MQ=255)
                                                 gATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGActc                                             >  2:2561340/1‑100 (MQ=255)
                                                  aTTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGActct                                            <  1:585264/100‑1 (MQ=255)
                                                  aTTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGActct                                            <  1:1199170/100‑1 (MQ=255)
                                                   ttCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACtctc                                           >  2:2120107/1‑100 (MQ=255)
                                                   ttCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACtctc                                           >  2:1445001/1‑100 (MQ=255)
                                                     cGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCAt                                         <  2:2764908/100‑1 (MQ=255)
                                                     cGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCAt                                         <  2:2797159/100‑1 (MQ=255)
                                                      ggCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATg                                        <  1:1517853/100‑1 (MQ=255)
                                                      ggCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATg                                        <  1:843191/100‑1 (MQ=255)
                                                         gTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGt                                     >  2:141418/1‑100 (MQ=255)
                                                           ttCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTa                                   <  1:2692934/100‑1 (MQ=255)
                                                            tCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTGTCATGAGTTa                                   >  1:2420449/1‑99 (MQ=255)
                                                             cGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACGCAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACg                                 >  2:1595843/1‑100 (MQ=255)
                                                             cGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACg                                 <  1:1057328/100‑1 (MQ=255)
                                                             cGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACg                                 >  1:522335/1‑100 (MQ=255)
                                                              gTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGt                                >  1:1266283/1‑100 (MQ=255)
                                                              gTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGt                                <  2:2204220/100‑1 (MQ=255)
                                                              gTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGt                                >  1:447934/1‑100 (MQ=255)
                                                                 aTTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTcc                             >  2:2077262/1‑100 (MQ=255)
                                                                   tttACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCgg                           <  1:778023/100‑1 (MQ=255)
                                                                     tACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGAc                         >  1:2359754/1‑100 (MQ=255)
                                                                     tACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGAc                         >  1:2360098/1‑100 (MQ=255)
                                                                       ccGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTg                       <  2:522340/100‑1 (MQ=255)
                                                                       ccGCTCCTGGTAAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCGTTACACTGTTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGAc                         <  1:1091050/98‑1 (MQ=255)
                                                                        cGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTgg                      <  1:2068354/100‑1 (MQ=255)
                                                                        cGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTgg                      <  1:2530098/100‑1 (MQ=255)
                                                                               ggTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAAt               <  2:1878360/100‑1 (MQ=255)
                                                                                gTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATg              <  2:2538071/100‑1 (MQ=255)
                                                                                gTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATg              <  2:1780327/100‑1 (MQ=255)
                                                                                 tCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATgg             >  2:888407/1‑100 (MQ=255)
                                                                                 tCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATgg             >  2:1912864/1‑100 (MQ=255)
                                                                                  cAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGc            >  1:2243472/1‑100 (MQ=255)
                                                                                   aaTATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCt           >  1:1394847/1‑100 (MQ=255)
                                                                                    atatTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTg          >  1:2849692/1‑100 (MQ=255)
                                                                                    atatTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTg          <  1:961192/100‑1 (MQ=255)
                                                                                     tatTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGa         >  1:1292861/1‑100 (MQ=255)
                                                                                      atTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAg        <  1:1431655/100‑1 (MQ=255)
                                                                                       ttttGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGt       <  2:2626844/100‑1 (MQ=255)
                                                                                         ttgttgATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTa     <  1:1044454/100‑1 (MQ=255)
                                                                                          tgttgATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTAt    >  2:637884/1‑100 (MQ=255)
                                                                                          tgttgATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTAt    >  1:266773/1‑100 (MQ=255)
                                                                                           tggggaggggggCGCGTTGTGGGGGGTCTTGACACAGCGGGATGTCTGGAGCACGAGTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAg        <  1:2642286/90‑1 (MQ=255)
                                                                                           gttgATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATc   >  2:814850/1‑100 (MQ=255)
                                                                                           gttgATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATc   <  2:2572155/100‑1 (MQ=255)
                                                                                            ttgATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCt  <  2:1564358/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCT  >  NC_000913/2531494‑2531686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: