Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,277,706 (A)7→6 coding (370/465 nt) yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,7000A.98.0% 444.4 / 1.6 101coding (364/465 nt)yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (44/55);  total (45/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 118 total aligned reads displayed.

GGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACC  >  NC_000913/3277601‑3277795
                                                                                                   |                                                                                               
ggTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTa                                                                                                 <  1:1614739/100‑1 (MQ=255)
  tGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaa                                                                                               >  1:1496058/1‑100 (MQ=255)
   gCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaa                                                                                              <  2:1730204/100‑1 (MQ=255)
   gCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaa                                                                                              <  2:1496082/100‑1 (MQ=255)
    cTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaa                                                                                             >  1:2264511/1‑100 (MQ=255)
    cTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaa                                                                                             <  1:1385579/100‑1 (MQ=255)
     tGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaa                                                                                            >  2:120986/1‑100 (MQ=255)
      ggTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                           <  2:1323054/100‑2 (MQ=255)
      ggTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                           >  2:644793/1‑99 (MQ=255)
      ggTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                           >  1:1299062/1‑99 (MQ=255)
       gTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                          <  1:1304625/100‑3 (MQ=255)
       gTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                          <  2:297169/100‑3 (MQ=255)
        tCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaacag                                                                                         <  2:145246/100‑4 (MQ=255)
        tCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaacag                                                                                         <  2:82366/100‑4 (MQ=255)
          gTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGAt                                                                                      <  1:1167042/100‑1 (MQ=255)
             aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGGAAACACTCTGCGCACCTACGGTAAAAAACAGATGcc                                                                                   <  2:705343/100‑1 (MQ=255)
             aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGcc                                                                                   >  1:1166316/1‑100 (MQ=255)
             aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGcc                                                                                   <  1:1880144/100‑1 (MQ=255)
             aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGcc                                                                                   <  1:389762/100‑1 (MQ=255)
               cGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCta                                                                                 >  1:450127/1‑100 (MQ=255)
                gTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCtat                                                                                >  2:2478790/1‑100 (MQ=255)
                  ctctTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATAc                                                                              <  2:1889489/100‑1 (MQ=255)
                      tcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTa                                                                          <  1:1277552/100‑1 (MQ=255)
                       cttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTAt                                                                         >  1:840481/1‑100 (MQ=255)
                       cttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTAt                                                                         >  1:731521/1‑100 (MQ=255)
                       cttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTAt                                                                         >  2:2084725/1‑100 (MQ=255)
                        tttCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTAtt                                                                        <  2:945181/100‑1 (MQ=255)
                         ttCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTc                                                                       <  1:863256/100‑1 (MQ=255)
                          tCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCa                                                                      <  2:573844/100‑1 (MQ=255)
                          tCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCa                                                                      >  1:2111273/1‑100 (MQ=255)
                           cGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAg                                                                     >  1:753625/1‑100 (MQ=255)
                            gTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCGTATACCGTATTCAGc                                                                    >  2:2864157/1‑100 (MQ=255)
                               ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCaaa                                                                 <  2:1948479/100‑1 (MQ=255)
                                 aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAAt                                                               <  1:30028/100‑1 (MQ=255)
                                 aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAAt                                                               >  1:2629683/1‑100 (MQ=255)
                                 aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAAt                                                               >  1:291170/1‑100 (MQ=255)
                                  ctGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATg                                                              <  2:1313504/99‑1 (MQ=255)
                                   tGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGt                                                             >  2:325337/1‑100 (MQ=255)
                                    gAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAACATGtt                                                            >  1:827658/1‑100 (MQ=255)
                                    gAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATgag                                                            >  1:1313486/1‑98 (MQ=255)
                                    gAAAAAGAGAAAGACATCATTCTGGGATGGATGAACGATGAAAACACACTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGtt                                                            >  1:1064393/1‑100 (MQ=255)
                                     aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTa                                                           <  2:1062304/100‑1 (MQ=255)
                                     aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTa                                                           <  2:253945/100‑1 (MQ=255)
                                      aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaa                                                          >  1:1438162/1‑100 (MQ=255)
                                        aaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaa                                                        >  1:2091874/1‑100 (MQ=255)
                                          gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAag                                                      <  1:456368/100‑1 (MQ=255)
                                          gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAag                                                      <  2:1446359/100‑1 (MQ=255)
                                          gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAag                                                      >  1:2811701/1‑100 (MQ=255)
                                          gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAag                                                      >  1:1615934/1‑100 (MQ=255)
                                           agaAAGTCATCATTCTAGGATGGATGAACGATGAAAACAATCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAAAAAAATATTAAAAaga                                                     <  1:1353096/100‑1 (MQ=255)
                                            gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAagag                                                    <  1:2827337/100‑1 (MQ=255)
                                             aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAgg                                                   <  2:2653318/100‑1 (MQ=255)
                                             aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAgg                                                   <  1:747856/100‑1 (MQ=255)
                                             aaaGTCATCATTCTGGGAGGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGACGCCTATACCGTATTCAGCAAAATGTTAAAAAGAgg                                                   <  1:880036/100‑1 (MQ=255)
                                              aaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGa                                                  <  2:384105/100‑1 (MQ=255)
                                              aaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGa                                                  <  2:536444/100‑1 (MQ=255)
                                              aaGTCATCATTCTGGGATAGATGAAAGATGAAAACACTCTGCGAAAAAACAAAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGa                                                  <  1:2136705/100‑1 (MQ=255)
                                               aGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGAc                                                 <  2:2811737/100‑1 (MQ=255)
                                                   atcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcc                                             <  1:2248773/100‑1 (MQ=255)
                                                   atcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcc                                             <  1:2758449/100‑1 (MQ=255)
                                                   atcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACATACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcc                                             <  1:1830530/100‑1 (MQ=255)
                                                     catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctc                                           >  2:413138/1‑100 (MQ=255)
                                                     catTCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAACAGATACCTATAACGTATCCAGCAAAATGTTAAAAAGAGGCCAt                                               >  1:1161700/1‑96 (MQ=255)
                                                      atTCTGGGATGGATGAACGATGAAAACACTCTGCGCACGTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctcc                                          <  1:1579529/100‑1 (MQ=255)
                                                      atTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctcc                                          <  2:98950/100‑1 (MQ=255)
                                                      atTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctcc                                          <  1:696910/100‑1 (MQ=255)
                                                             gatggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGAc                                   >  2:2206625/1‑100 (MQ=255)
                                                             gatggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAATGTTAAAAAGAGGACATCCTCCTGCCGACt                                  >  2:2099247/1‑100 (MQ=255)
                                                               tggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTg                                 <  1:2688386/100‑1 (MQ=255)
                                                                ggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTgg                                <  2:585360/100‑1 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                               <  1:1799429/100‑1 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                               <  1:649749/100‑1 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                               >  2:1851495/1‑100 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                               >  1:1264707/1‑100 (MQ=255)
                                                                 gatgAACGATGAAAACAAACTGCGCACCTGCGGTAAAAGACAAATGCCTATACAGTATTCAGCAAAATGTTTAAAAGACGACATCCTCCTGCCGGCTgg                                >  1:1729369/1‑99 (MQ=255)
                                                                  atgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGa                              <  2:446241/100‑1 (MQ=255)
                                                                   tgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                               <  1:951185/98‑1 (MQ=255)
                                                                   tgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa                             >  1:3873/1‑100 (MQ=255)
                                                                    gAACGATGAAAACACTCTGCGCACCTACGGTAAAAACCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaaa                            >  1:1650127/1‑100 (MQ=255)
                                                                      aCGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAcc                          >  1:2670807/1‑100 (MQ=255)
                                                                       cGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                         <  2:2421449/100‑1 (MQ=255)
                                                                       cGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAcc                          <  2:1299080/99‑1 (MQ=255)
                                                                        gATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCGAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                        >  1:1859143/1‑100 (MQ=255)
                                                                        gATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                        <  1:543701/100‑1 (MQ=255)
                                                                        gATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                        <  1:1419186/100‑1 (MQ=255)
                                                                            aaaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                    <  1:1101182/100‑1 (MQ=255)
                                                                            aaaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                    >  2:2178113/1‑100 (MQ=255)
                                                                             aaaCACTCTGCGCACCTACGGTAAAAACACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                    >  1:1112067/1‑100 (MQ=255)
                                                                             aaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAccc                   >  1:2521836/1‑100 (MQ=255)
                                                                             aaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAccc                   >  1:1918243/1‑100 (MQ=255)
                                                                              aaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                  <  1:120983/100‑1 (MQ=255)
                                                                                cacTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgag                >  1:91448/1‑100 (MQ=255)
                                                                                  ctctGCGCACATACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaa              <  1:1903240/100‑1 (MQ=255)
                                                                                    ctGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc            <  1:1580659/100‑1 (MQ=255)
                                                                                      gcgcACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACag          <  2:1264725/100‑1 (MQ=255)
                                                                                       cgcACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACaga         <  2:714577/100‑1 (MQ=255)
                                                                                         cACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaag       <  2:2810246/100‑1 (MQ=255)
                                                                                         cACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaag       >  1:895641/1‑100 (MQ=255)
                                                                                            cTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaaga      <  1:486425/98‑1 (MQ=255)
                                                                                             tACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAc   >  2:2904395/1‑100 (MQ=255)
                                                                                                   |                                                                                               
GGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACC  >  NC_000913/3277601‑3277795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: