Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,774 (T)7→8 coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7671.T98.2% 374.8 / ‑0.6 110coding (151/738 nt)ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (45/63);  total (46/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 129 total aligned reads displayed.

GCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGA  >  NC_000913/2809672‑2809860
                                                                                                 |                                                                                              
gCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑tttt                                                                                           <  2:979133/100‑1 (MQ=255)
 cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttt                                                                                          <  1:2177025/100‑1 (MQ=255)
   aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttt                                                                                        <  2:1623075/100‑1 (MQ=255)
   aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttt                                                                                        <  1:655437/100‑1 (MQ=255)
   aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttt                                                                                        >  2:677887/1‑100 (MQ=255)
    aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑tttttttt                                                                                       <  2:2819494/100‑2 (MQ=255)
     gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttttc                                                                                      <  1:2726959/100‑3 (MQ=255)
     gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttttc                                                                                      <  2:1700238/100‑3 (MQ=255)
      aCAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttttct                                                                                     >  2:863829/1‑97 (MQ=255)
       cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttttctc                                                                                    <  1:2839367/100‑5 (MQ=255)
       cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttttctc                                                                                    >  1:600033/1‑96 (MQ=255)
        aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑ttttttttctcc                                                                                   <  1:2780663/100‑6 (MQ=255)
         gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCt                                                                                   >  1:1389192/1‑100 (MQ=255)
         gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCt                                                                                   >  1:1215647/1‑100 (MQ=255)
          tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTg                                                                                  <  2:1926385/100‑1 (MQ=255)
            tACCGATTGTTATTATTGATATTCCGGTGGCCTTGGCGTTCTGTCTGACTTAGTCACATCTGAGATTAGCACCCCTCGAAAGC‑G‑‑ttttttttctct                                                                                   <  1:128800/96‑6 (MQ=255)
            tACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGca                                                                                <  1:847987/100‑1 (MQ=255)
             aCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGcat                                                                               >  1:1293267/1‑100 (MQ=255)
              ccGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGcatc                                                                              <  2:1548132/100‑1 (MQ=255)
               cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGcatca                                                                             <  2:2476019/100‑1 (MQ=255)
               cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGcatc                                                                              <  1:273500/99‑1 (MQ=255)
                gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGcatcat                                                                            >  2:2330627/1‑100 (MQ=255)
                gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGcatcat                                                                            >  2:2894178/1‑100 (MQ=255)
                  ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCAttt                                                                          <  2:13430/100‑1 (MQ=255)
                   tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTa                                                                         <  1:263479/100‑1 (MQ=255)
                   tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTa                                                                         <  2:6548/100‑1 (MQ=255)
                   tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTa                                                                         <  1:98086/100‑1 (MQ=255)
                    gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTAt                                                                        <  2:2599774/100‑1 (MQ=255)
                    gTTATTAGTTATATTCCGGTGGACTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGAGTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTAt                                                                        >  1:1692822/1‑100 (MQ=255)
                     ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATg                                                                       <  1:1494000/100‑1 (MQ=255)
                     ntattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑TTTTTTTCTCCTGCATCATTTATGc                                                                      >  2:2772184/2‑100 (MQ=255)
                      tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGc                                                                      >  2:1142135/1‑100 (MQ=255)
                      tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGc                                                                      <  2:563074/100‑1 (MQ=255)
                         taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAgg                                                                   >  2:261549/1‑100 (MQ=255)
                         taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAgg                                                                   <  1:312749/100‑1 (MQ=255)
                          aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGTCCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATAAATGATGCAgga                                                                  <  1:1660636/100‑2 (MQ=255)
                          aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGgc                                                                  >  1:1037345/1‑100 (MQ=255)
                          aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGgc                                                                  >  2:80746/1‑100 (MQ=255)
                          aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCGCCTGCATAAATGATGCAgga                                                                  <  2:2638010/100‑2 (MQ=255)
                          aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCGCCTGCATAAATGATGCAgga                                                                  <  2:2811462/100‑2 (MQ=255)
                           gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGgcg                                                                 <  1:677881/100‑1 (MQ=255)
                            ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATAAATGATGCAggaga                                                                <  2:1687711/100‑4 (MQ=255)
                             tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGcgcg                                                               <  2:302764/100‑1 (MQ=255)
                             tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATAAATGATGCAggagaa                                                               <  2:1279267/100‑5 (MQ=255)
                               tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAg                                                             <  2:1672863/100‑1 (MQ=255)
                                nttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                            >  2:1345477/2‑100 (MQ=255)
                                 ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                           <  1:746955/100‑1 (MQ=255)
                                 ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                           <  2:1582851/100‑1 (MQ=255)
                                 ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                           <  2:1106810/100‑1 (MQ=255)
                                        ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGt                                                    >  1:1485936/1‑100 (MQ=255)
                                        ggCCTTTGCGTTCGGTCTGAATGCGACCCGGCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                     <  1:1118119/99‑1 (MQ=255)
                                          ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                  <  2:2849639/100‑1 (MQ=255)
                                           cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                 <  1:2575790/100‑1 (MQ=255)
                                           cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                 <  2:1452746/100‑1 (MQ=255)
                                           cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                 >  1:2486804/1‑100 (MQ=255)
                                           cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                 >  2:2090711/1‑100 (MQ=255)
                                             ttGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTa                                               <  2:2726044/100‑1 (MQ=255)
                                              tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc                                              >  2:2775297/1‑100 (MQ=255)
                                                cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                            >  2:253967/1‑100 (MQ=255)
                                                cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                            >  2:190029/1‑100 (MQ=255)
                                                 gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                           <  1:2767081/100‑1 (MQ=255)
                                                   tCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGa                                         <  2:2351536/100‑1 (MQ=255)
                                                   tCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGa                                         <  1:863823/100‑1 (MQ=255)
                                                     ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                       <  1:261545/100‑1 (MQ=255)
                                                     ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                       >  2:1759012/1‑100 (MQ=255)
                                                      gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc                                      >  2:1565381/1‑100 (MQ=255)
                                                        cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCAT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                    <  1:2215596/100‑1 (MQ=255)
                                                         tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                   >  1:899816/1‑100 (MQ=255)
                                                         tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                   <  2:1037358/100‑1 (MQ=255)
                                                          gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                  <  1:2341487/100‑1 (MQ=255)
                                                            aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                                >  2:122387/1‑100 (MQ=255)
                                                             tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                 >  1:419150/1‑98 (MQ=255)
                                                              gCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGcc                              <  1:1626123/100‑1 (MQ=255)
                                                               cGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GTTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGcc                              >  2:2558787/1‑100 (MQ=255)
                                                               cGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCCGGCAGCCg                             <  2:2752822/100‑1 (MQ=255)
                                                                gACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCTCGAGCCAGTTCGTCATTACCGCGATGCTGTTAGCCgg                            >  1:370707/1‑100 (MQ=255)
                                                                gACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                            <  2:600039/100‑1 (MQ=255)
                                                                 aCCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                           <  2:1086758/100‑1 (MQ=255)
                                                                    cGTCTGGGATGCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                        <  1:1949328/100‑1 (MQ=255)
                                                                       cTGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtagt                     >  2:541872/1‑100 (MQ=255)
                                                                        tGGGATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTTTTTTTTCATTTATACAAGCGCGAGCAAGTTCGGCATTACCGCGATGCTGGCAGCCGGGagtatt                     >  1:272445/1‑97 (MQ=255)
                                                                         gggATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTTCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtggt                     >  1:1468419/1‑98 (MQ=255)
                                                                           gATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt                 <  2:1485960/100‑1 (MQ=255)
                                                                           gATTCTCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt                 <  2:582993/100‑1 (MQ=255)
                                                                           gATTCCCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGCAGTAGTTtgt                 >  2:628223/1‑100 (MQ=255)
                                                                             ttCCCTCCTCTCGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt                 <  2:392415/98‑1 (MQ=255)
                                                                              tctctcCTCTTGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGa              <  1:2780809/100‑1 (MQ=255)
                                                                                tctcctctcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt            <  2:2186057/100‑1 (MQ=255)
                                                                                 ctcctctcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg           >  1:451832/1‑100 (MQ=255)
                                                                                  tcctctcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc          >  2:1716713/1‑100 (MQ=255)
                                                                                  tcctctcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc          >  2:2128444/1‑100 (MQ=255)
                                                                                  gtatctcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc          <  1:1364048/97‑1 (MQ=255)
                                                                                    ctctcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        <  1:1349660/100‑1 (MQ=255)
                                                                                     tctcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc       <  1:402679/100‑1 (MQ=255)
                                                                                      ctcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGGGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc       <  1:1423362/99‑1 (MQ=255)
                                                                                       tcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc     <  1:223733/100‑1 (MQ=255)
                                                                                       tcGAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc     <  2:1810173/100‑1 (MQ=255)
                                                                                        cGAGAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGGGGGCCAGTTCGTTATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgatgc       <  1:1423504/97‑1 (MQ=255)
                                                                                        cGAAAGC‑AT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACt    >  2:1529887/1‑100 (MQ=255)
                                                                                         gAAAGC‑GT‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg   <  1:1142117/100‑1 (MQ=255)
                                                                                                 |                                                                                              
GCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGA  >  NC_000913/2809672‑2809860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑