BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	94.6%	368.9 / 13.1	112	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (3/3); new base C (47/59); total (50/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGt‑tt                                                                                                     >  2:1094534/1‑100 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc                                                                                                 >  1:805228/1‑100 (MQ=255)

     aGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑c                                                                                            >  2:1915206/1‑100 (MQ=255)

      gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑cc                                                                                           <  2:2112696/100‑1 (MQ=255)

      gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑cc                                                                                           >  1:431870/1‑100 (MQ=255)

       cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑cc‑c                                                                                         <  1:1829815/100‑1 (MQ=255)

         tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑cc‑ccg                                                                                       <  1:2407886/100‑3 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑cc‑ccgcc                                                                                     >  2:566052/1‑96 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑cc‑ccgcc                                                                                     <  1:1915179/100‑5 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑cc‑ccgcc                                                                                     >  2:2141189/1‑96 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAg                                                                                    >  2:1063709/1‑100 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAg                                                                                    >  1:1849268/1‑100 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑CC‑CC‑CC‑CGCCAgc                                                                                   >  2:1948879/1‑100 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑cc‑cc‑cc‑ccgcccgcg                                                                                 >  1:728170/1‑91 (MQ=255)

                  agaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCa                                                                               <  2:1827638/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTATTGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAAcc                                                                            <  2:2036524/100‑1 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAAcc                                                                            <  1:900620/98‑1 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCAt                                                                          <  2:32642/100‑1 (MQ=255)

                        ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATa                                                                         <  1:1099113/100‑1 (MQ=255)

                         cAGCGGCTGGGTCTCGACCCCCCATGTTTCCAATTGTCGATGGGTGGATTCCAACCTCCGGTAAATTTTCCGGT‑TCC‑CC‑CC‑CC‑CGCCAGCGCCAAc                                                                             <  1:1623062/96‑1 (MQ=255)

                          gtcgagaacttCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGt                                                                       >  1:1023365/10‑100 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTATCGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGtt                                                                      <  2:2010726/100‑1 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGtt                                                                      <  1:2192755/100‑1 (MQ=255)

                            cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGttt                                                                     <  1:1164325/100‑1 (MQ=255)

                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCCTAGTTTAt                                                                   >  1:746827/1‑100 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATcc                                                                 >  1:1278264/1‑100 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACCC‑CCGGGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATc                                                                  <  1:480883/99‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCa                                                                <  1:1799439/100‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCa                                                                <  2:2539050/100‑1 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATccct                                                               >  2:928547/1‑98 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGACCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCAt                                                               >  2:1766930/1‑100 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCTGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  1:1597044/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:2103472/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:2163424/100‑1 (MQ=255)

                                      tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTgc                                                           >  1:920117/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTTATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTCCCC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:2206028/99‑1 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  1:2190513/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGAGCCAACCATAGTTTATCAATTTgcg                                                          >  1:2458040/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑CC‑CCACC‑CGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  1:1534996/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGAAGGTTGCTTTCTAACGT‑CGGTGAATTTTCCCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGCTTCTCGATTTgc                                                           >  1:2247485/1‑99 (MQ=255)

                                         aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                        <  2:2398484/100‑1 (MQ=255)

                                         aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                        >  2:2286185/1‑100 (MQ=255)

                                           gCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGC‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCg                                                      >  2:259626/1‑100 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                     >  1:2394969/1‑100 (MQ=255)

                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                >  1:829672/1‑100 (MQ=255)

                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                <  1:1241698/100‑1 (MQ=255)

                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                >  2:2052416/1‑100 (MQ=255)

                                                    tgCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  2:2421331/1‑100 (MQ=255)

                                                    tgCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  1:1313566/1‑100 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  1:522039/100‑1 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                         <  2:155432/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     >  1:2204412/1‑100 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:1702703/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TC‑‑CCTCC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  1:2042825/100‑1 (MQ=255)

                                                              cGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   <  2:1023376/100‑1 (MQ=255)

                                                              cGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   >  1:1687127/1‑100 (MQ=255)

                                                              cGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   <  2:1269233/100‑1 (MQ=255)

                                                                    ttGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             >  1:1300602/1‑100 (MQ=255)

                                                                    ttGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             >  2:2592564/1‑100 (MQ=255)

                                                                      gATTTCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCca                           <  1:2140826/100‑1 (MQ=255)

                                                                        tttCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  1:1064312/100‑1 (MQ=255)

                                                                        tttCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  2:888292/100‑1 (MQ=255)

                                                                         ttCTAACGT‑CGGTGAATTTTCTCGTGTC‑‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGGTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                        <  1:1208022/100‑1 (MQ=255)

                                                                         ttCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                        <  2:746832/100‑1 (MQ=255)

                                                                         ttCTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                        >  1:1902310/1‑100 (MQ=255)

                                                                         ttCTAACGT‑CGGTGAATATTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                        <  2:1936934/100‑1 (MQ=255)

                                                                           cTAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                      <  2:1858578/100‑1 (MQ=255)

                                                                            tAACGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                     >  2:1910713/1‑100 (MQ=255)

                                                                            tAACGT‑CGGTGAATTTTCTCGT‑TT‑‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                    >  2:369794/1‑100 (MQ=255)

                                                                             aaCGT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                    <  2:1350694/100‑1 (MQ=255)

                                                                              aCGT‑CGGTGAATTTTCTCGT‑TCC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                     <  1:1026639/98‑1 (MQ=255)

                                                                                gT‑CGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                 <  2:2190540/100‑1 (MQ=255)

                                                                                   cGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               >  1:2811539/1‑100 (MQ=255)

                                                                                   cGGTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               <  2:575902/100‑1 (MQ=255)

                                                                                     gTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             >  2:1650675/1‑100 (MQ=255)

                                                                                     gTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  1:2769711/100‑1 (MQ=255)

                                                                                     gTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  1:1043240/100‑1 (MQ=255)

                                                                                     gTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             >  2:43681/1‑100 (MQ=255)

                                                                                     gTGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             >  2:2762625/1‑100 (MQ=255)

                                                                                      tGAATTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt            <  2:1849294/100‑1 (MQ=255)

                                                                                        aaTTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtga          <  2:2217894/100‑1 (MQ=255)

                                                                                         aTTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  2:1393739/100‑1 (MQ=255)

                                                                                         aTTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  2:862779/1‑100 (MQ=255)

                                                                                         aTTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:885237/100‑1 (MQ=255)

                                                                                         aTTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  2:2245888/100‑1 (MQ=255)

                                                                                         aTTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  1:1895391/1‑100 (MQ=255)

                                                                                         aTTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  1:2273759/1‑100 (MQ=255)

                                                                                         aTTTTCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCCCCCGCGTTTCATCCTGAAGTGGCTAAATCCACAGATGCGCCtgattgag         >  1:2423467/1‑100 (MQ=255)

                                                                                          ttttCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt        <  1:1391459/100‑1 (MQ=255)

                                                                                           tttCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGTTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  2:728176/98‑1 (MQ=255)

                                                                                           tttCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg       <  2:829678/100‑1 (MQ=255)

                                                                                           tttCTCGT‑TT‑‑CC‑CC‑CCTCGCCAGCGCCAACCATAGTTTATCCAGTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:2893635/98‑1 (MQ=255)

                                                                                            ttCTCGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt      >  2:1980143/1‑100 (MQ=255)

                                                                                             tctcGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa     >  1:566551/1‑100 (MQ=255)

                                                                                             tctcGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa     >  1:1398555/1‑100 (MQ=255)

                                                                                             tctcGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa     <  2:1826344/100‑1 (MQ=255)

                                                                                               tcGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg   <  2:1202047/100‑1 (MQ=255)

                                                                                                cGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGACGTGGCGAAAACCACAGATGCGCCTGAGTGAGTGTACg   >  1:542742/1‑99 (MQ=255)

                                                                                                cGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  <  2:2449292/100‑1 (MQ=255)

                                                                                                cGT‑TTC‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  >  1:1870730/1‑100 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGT‑CGGTGAATTTTCTCGT‑TT‑‑CC‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`