Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,8780TA25.2% 197.6 / 71.5 108intergenic (+20/+29)slyX/slyDphi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base T (34/46);  new base A (10/17);  total (44/64)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 110 total aligned reads displayed.

GCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGT  >  NC_000913/3477780‑3477974
                                                                                                  |                                                                                                
gctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAtt                                                                                                 >  2:2293157/1‑100 (MQ=255)
gctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAtt                                                                                                 >  2:2404606/1‑100 (MQ=255)
   gACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTccc                                                                                              >  2:1564388/1‑100 (MQ=255)
   gACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTccc                                                                                              <  1:1814089/100‑1 (MQ=255)
     ccGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCg                                                                                            >  2:1369262/1‑100 (MQ=255)
      cGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGc                                                                                           >  1:2359698/1‑100 (MQ=255)
       gagaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCt                                                                                          >  1:379087/1‑100 (MQ=255)
         gaAGTTAAAAGCCAGTCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttt                                                                                        <  2:2865562/100‑1 (MQ=255)
         gaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttt                                                                                        <  2:2217070/100‑1 (MQ=255)
         gaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttt                                                                                        <  2:2447285/100‑1 (MQ=255)
          ttccgatctgccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttt                                                                                       <  2:2659934/91‑1 (MQ=255)
          aaGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttt                                                                                       <  1:1876258/100‑1 (MQ=255)
          aaGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCACtttt                                                                                       <  1:516324/100‑1 (MQ=255)
           aGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                      <  2:1714852/100‑1 (MQ=255)
           aGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttttt                                                                                      <  2:1000455/100‑1 (MQ=255)
           aGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttttt                                                                                      <  1:1226850/100‑1 (MQ=255)
           aGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttttt                                                                                      >  2:1633357/1‑100 (MQ=255)
            gTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCtttttt                                                                                     >  2:2155451/1‑100 (MQ=255)
            gTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttttt                                                                                     <  1:1042276/100‑1 (MQ=255)
            gTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttttt                                                                                     <  2:1643432/100‑1 (MQ=255)
            gTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttttt                                                                                     <  1:1633332/100‑1 (MQ=255)
             ttAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCCCCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTg                                                                                    <  1:343735/100‑1 (MQ=255)
             ttAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTg                                                                                    <  1:753555/100‑1 (MQ=255)
             ttAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttttttt                                                                                    <  2:1075587/100‑2 (MQ=255)
              tAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGt                                                                                   >  1:607370/1‑100 (MQ=255)
               aaaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTc                                                                                  <  1:2259483/100‑1 (MQ=255)
               aaaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTc                                                                                  <  2:1644548/100‑1 (MQ=255)
               aaaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTc                                                                                  >  1:2055290/1‑100 (MQ=255)
               aaaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttttttac                                                                                  <  2:2723978/100‑4 (MQ=255)
               aaaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttttttac                                                                                  <  2:423934/100‑4 (MQ=255)
               aaaaGCCAGCCAGCCGGCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttttttta                                                                                   <  2:345061/99‑3 (MQ=255)
                aaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCa                                                                                 <  2:926708/100‑1 (MQ=255)
                aaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCa                                                                                 >  1:1057161/1‑100 (MQ=255)
                 aaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttttttacgc                                                                                <  1:2227083/100‑6 (MQ=255)
                 aaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttttttacg                                                                                 <  2:2844331/99‑5 (MQ=255)
                  agccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACt                                                                               >  1:832308/1‑100 (MQ=255)
                  agccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtttttttacgcc                                                                               <  2:410967/100‑7 (MQ=255)
                   gccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACtt                                                                              <  2:1889864/100‑1 (MQ=255)
                   gccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACtt                                                                              >  2:2487418/1‑100 (MQ=255)
                   gccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttttagatcggaa                                                                              >  1:2659898/1‑91 (MQ=255)
                    ccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACttt                                                                             <  2:2566631/100‑1 (MQ=255)
                     cagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACtttt                                                                            <  2:2435760/100‑1 (MQ=255)
                       gccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTc                                                                          <  2:1721181/100‑1 (MQ=255)
                       gccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTc                                                                          >  2:1259777/1‑100 (MQ=255)
                        ccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCg                                                                         >  1:2085303/1‑100 (MQ=255)
                         cagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCgg                                                                        <  2:810156/100‑1 (MQ=255)
                          agccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGt                                                                       <  2:780957/100‑1 (MQ=255)
                          agccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGt                                                                       <  1:591665/100‑1 (MQ=255)
                           ggcGTCGAACATTGAGTCGCAGGCTGAAGAAACTCAACCGCCACATTATTGAGGCGTAAAAGAAGGGGGGATTCCCCGCTTTTTTGTCCCTTTTTCGGTa                                                                      <  1:916890/98‑1 (MQ=255)
                           gccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTa                                                                      <  1:729966/100‑1 (MQ=255)
                           gccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTa                                                                      <  2:1798664/100‑1 (MQ=255)
                            gcGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGACGCGTAAAAAAAGCGGGGAGTCCCCGCTTTTTTGTCACTTTGTCGGTa                                                                      <  1:2441848/98‑1 (MQ=255)
                            ccGTCGAACATCGCGTCGCAGGCTTAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTa                                                                      <  1:1187149/99‑1 (MQ=255)
                              gTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTa                                                                   >  1:140296/1‑100 (MQ=255)
                              gTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTa                                                                   <  1:2178221/100‑1 (MQ=255)
                               tCGAACATCGCGTCGCAGGCTGAAGAAGCGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAg                                                                  <  2:2671502/100‑1 (MQ=255)
                               tCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAg                                                                  <  1:1065826/100‑1 (MQ=255)
                               tCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAg                                                                  >  2:543744/1‑100 (MQ=255)
                                 gAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTg                                                                >  2:517518/1‑100 (MQ=255)
                                 gAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTg                                                                <  1:1117455/100‑1 (MQ=255)
                                  aaCATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTgg                                                               >  2:1808983/1‑100 (MQ=255)
                                     aTCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgca                                                             <  1:2359117/99‑1 (MQ=255)
                                      tCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaac                                                           <  2:489188/100‑1 (MQ=255)
                                      tCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaac                                                           <  2:1981976/100‑1 (MQ=255)
                                       cgcgTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaacc                                                          <  2:1226418/100‑1 (MQ=255)
                                         cgTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaaccgc                                                        <  2:2491345/100‑1 (MQ=255)
                                          gTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaaccgca                                                       <  1:1259759/100‑1 (MQ=255)
                                              cAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGcaaccgcaaccg                                                   >  1:652405/1‑100 (MQ=255)
                                                ggCTGAAGAAACGCCACCGCTACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAAccgcc                                                 >  1:1773180/1‑100 (MQ=255)
                                                 gCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAAccgccg                                                <  1:1483243/100‑1 (MQ=255)
                                                     aagaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGc                                            >  2:2354228/1‑100 (MQ=255)
                                                       gaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCt                                          <  1:437125/100‑1 (MQ=255)
                                                            gccaccgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTAcc                                     <  1:307461/100‑1 (MQ=255)
                                                            gccaccgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTAcc                                     <  2:1040845/100‑1 (MQ=255)
                                                                 cgccacGTTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCcac                                <  2:1948720/100‑1 (MQ=255)
                                                                 cgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCcac                                <  2:2132468/100‑1 (MQ=255)
                                                                  gccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCcaca                               <  1:2082671/100‑1 (MQ=255)
                                                                    cacaTTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAcagc                             >  2:1920588/1‑100 (MQ=255)
                                                                     acaTTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAcagca                            >  2:607892/1‑100 (MQ=255)
                                                                          attGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCtt                       >  2:1121624/1‑100 (MQ=255)
                                                                           ttGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTTGTTTTTTTTTGCAACCGCAACCGCCGTTGCCTTTACCGCCTCAGCAGCCtt                       >  1:1879161/1‑99 (MQ=255)
                                                                              aGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGcc                   <  2:499743/100‑1 (MQ=255)
                                                                                gtgacaaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACAGCAACCGCCGTTGCCTTTACCGCCAcagca                            >  1:2683227/6‑89 (MQ=37)
                                                                                gCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCAc                 >  1:2889784/1‑100 (MQ=255)
                                                                                 cGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCAcc                <  1:2590973/100‑1 (MQ=255)
                                                                                  gTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTTTGTTTTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGTAGCCTTCGCCACCg               >  1:1796702/1‑100 (MQ=255)
                                                                                   tAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCgt              >  1:2135734/1‑100 (MQ=255)
                                                                                    caaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCgtg             >  1:1084731/2‑100 (MQ=255)
                                                                                    caaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCgtg             >  2:283866/2‑100 (MQ=255)
                                                                                    caaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCgtg             >  2:2642369/2‑100 (MQ=255)
                                                                                      aaaaaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGtt           <  2:484852/100‑1 (MQ=255)
                                                                                      aaaaaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGtt           <  2:607376/100‑1 (MQ=255)
                                                                                      aaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGtt           >  2:333397/1‑100 (MQ=255)
                                                                                      aaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGtt           >  2:380209/1‑100 (MQ=255)
                                                                                      aaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGtt           >  2:1616968/1‑100 (MQ=255)
                                                                                        aaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCa         >  2:2800709/1‑100 (MQ=255)
                                                                                          aGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATg       <  1:2404574/100‑1 (MQ=255)
                                                                                          aGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATg       <  1:501892/100‑1 (MQ=255)
                                                                                              gggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCg   <  2:2330468/100‑1 (MQ=255)
                                                                                               ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGt  <  2:338231/100‑1 (MQ=255)
                                                                                                  |                                                                                                
GCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGT  >  NC_000913/3477780‑3477974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑