Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9700GA35.3% 290.6 / 199.2 201intergenic (‑180/+128)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (66/64);  new base A (70/1);  total (136/65)
Fisher's exact test for biased strand distribution p-value = 1.73e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-01

Only 100 of 218 total aligned reads displayed.

CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069
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 aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                                                                                            >  2:1393026/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                           >  2:750776/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                           >  1:2163729/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                           >  1:1996054/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                           >  2:1549688/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                           >  2:1893230/1‑100 (MQ=255)
   ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGATGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                           <  1:2753011/99‑1 (MQ=255)
   ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                          <  2:1842474/100‑1 (MQ=255)
   ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                          >  1:2567862/1‑100 (MQ=255)
         aaGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                    <  1:836007/100‑1 (MQ=255)
          aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAc                                                                                                   <  2:44227/100‑1 (MQ=255)
           gTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                                  <  2:1775227/100‑1 (MQ=255)
           gTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                                  <  1:1186991/100‑1 (MQ=255)
             aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                <  2:1493413/100‑1 (MQ=255)
              ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                               >  1:858897/1‑100 (MQ=255)
               tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                              >  2:333279/1‑100 (MQ=255)
               tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                              >  2:697337/1‑100 (MQ=255)
                gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                             <  2:2819044/100‑1 (MQ=255)
                   gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                          >  2:375634/1‑100 (MQ=255)
                     cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                        >  1:2471602/1‑100 (MQ=255)
                     cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                        <  2:862823/100‑1 (MQ=255)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACAGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                     <  1:1063920/100‑1 (MQ=255)
                         tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                    <  2:2496524/100‑1 (MQ=255)
                          ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                   <  2:2592051/100‑1 (MQ=255)
                           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                 >  2:826200/1‑100 (MQ=255)
                              cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                              >  2:1723266/1‑100 (MQ=255)
                              cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                              >  2:1759176/1‑100 (MQ=255)
                              cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                              >  2:1299659/1‑100 (MQ=255)
                              cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcataaacaa                                                                               >  2:722757/1‑92 (MQ=25)
                                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                           <  1:1422990/100‑1 (MQ=255)
                                   cgtagagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                         >  1:1387172/4‑100 (MQ=255)
                                     cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                       >  2:2369323/1‑100 (MQ=255)
                                       gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaaa                                                                     >  2:1817733/1‑100 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                 >  2:1653039/1‑100 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                 >  2:2873943/1‑100 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                 >  1:675812/1‑100 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                 <  2:752611/100‑1 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                 <  1:1083665/100‑1 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                               <  1:2708076/100‑1 (MQ=255)
                                              aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                              <  1:712651/100‑1 (MQ=255)
                                              aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                              <  2:1102034/100‑1 (MQ=255)
                                                   cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                         >  2:495863/1‑100 (MQ=255)
                                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                   <  2:2690713/100‑1 (MQ=255)
                                                       gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGa                                                  >  1:1664997/1‑100 (MQ=255)
                                                          tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAtt                                             >  2:2769128/1‑100 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                       <  2:2575269/100‑1 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                       <  1:451959/100‑1 (MQ=255)
                                                               aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑Gt                                      >  1:2092942/1‑100 (MQ=255)
                                                                 aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAg                                    <  1:512197/100‑1 (MQ=255)
                                                                 aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAg                                    <  1:1022406/100‑1 (MQ=255)
                                                                   cataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGc                                  >  2:1263416/6‑100 (MQ=18)
                                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGcc                                 >  1:1525502/1‑100 (MQ=255)
                                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGcc                                 >  2:812691/1‑100 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCgg                       <  1:1450723/100‑1 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCgg                       <  1:225885/100‑1 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCg                        <  1:2232902/99‑1 (MQ=255)
                                                                                  cTTTGTTTATGCCGGAGGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                   <  2:626922/100‑1 (MQ=255)
                                                                                   attttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCATACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcgc                  >  2:2901299/10‑100 (MQ=18)
                                                                                    atttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCAt                >  2:1615108/9‑100 (MQ=18)
                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGCATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa              >  1:1220173/1‑100 (MQ=255)
                                                                                       gatctGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa              <  1:731614/96‑1 (MQ=255)
                                                                                        ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTAC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg             <  1:1546220/100‑1 (MQ=255)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAg             >  2:1787988/5‑100 (MQ=18)
                                                                                        gtaggCCGGATAAGGCGTTAACGCCGCATCCGGCATACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa              >  2:2697775/5‑99 (MQ=17)
                                                                                         taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg            >  1:1718905/4‑100 (MQ=17)
                                                                                         taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg            >  1:382737/4‑100 (MQ=17)
                                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAgg            >  2:1307194/4‑100 (MQ=21)
                                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg            >  2:2449185/4‑100 (MQ=21)
                                                                                          aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:2140386/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  2:2320983/3‑100 (MQ=21)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGC‑‑AGGATACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  2:2234509/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAACCGCGCAAATTCAATATATTGCGGAGAAAAT‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGct          >  2:2051672/3‑99 (MQ=18)
                                                                                          aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc           >  1:1821833/1‑100 (MQ=255)
                                                                                          aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc           >  2:512177/1‑100 (MQ=255)
                                                                                           tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa          >  2:22551/1‑100 (MQ=255)
                                                                                           tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa          <  2:2130947/100‑1 (MQ=255)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt         >  2:2196595/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:2209020/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGC‑‑AGAGATTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:1986094/2‑100 (MQ=14)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:1443462/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAAGCa          >  2:167696/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGC‑‑AGGAAACAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCa          >  1:894326/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:1344500/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt         >  2:2547557/2‑100 (MQ=21)
                                                                                           ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa          >  2:1132295/2‑100 (MQ=17)
                                                                                            gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  2:2730472/1‑100 (MQ=255)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaa         >  1:460059/1‑99 (MQ=21)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGA‑‑TCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag         >  1:166973/1‑99 (MQ=21)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa         >  1:2545427/1‑99 (MQ=18)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGC‑‑AGGATACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa         >  1:785572/1‑99 (MQ=18)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa         >  2:5827/1‑99 (MQ=17)
                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt        >  2:1078306/1‑100 (MQ=255)
                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt        >  1:2412885/1‑100 (MQ=255)
                                                                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCGTCAGGCATTtttg    <  1:1799991/100‑1 (MQ=255)
                                                                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg    <  1:1103607/100‑1 (MQ=255)
                                                                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg    <  1:697331/100‑1 (MQ=255)
                                                                                                  tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc   >  2:2099407/1‑100 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGTCTACAAAATCGTGCAAATTCAATACAGTGC‑‑AGAATTTTC‑‑GTAGGAATGATAAGCGGAGCGCATCAGGCATTtttgcc  <  2:2471634/100‑1 (MQ=18)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc  <  2:2113156/100‑1 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc  <  1:2367093/100‑1 (MQ=255)
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CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: