BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,706,017	0	C	T	30.2%	386.9 / 181.3	251	intergenic (‑227/+81)	dppB/dppA	dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand): ref base C (81/76); new base T (65/3); total (172/79)
Fisher's exact test for biased strand distribution p-value = 8.88e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 251 total aligned reads displayed.

GCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGT  >  NC_000913/3705920‑3706114

gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                                                       >  1:1582320/1‑100 (MQ=255)

  cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                                                                     >  2:495863/1‑100 (MQ=255)

      gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑Ga                                                                                                >  1:2857568/1‑100 (MQ=255)

      gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑Ga                                                                                                >  2:1800017/1‑100 (MQ=255)

         tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑At‑t                                                                                           >  2:2769128/1‑100 (MQ=255)

         tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑At‑t                                                                                           >  2:1449114/1‑100 (MQ=255)

           taCAGATTGCTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑At‑t‑tt                                                                                        >  1:1395319/1‑100 (MQ=255)

             cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑g                                                                                    <  2:2860928/100‑1 (MQ=255)

             cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑g                                                                                    <  1:451959/100‑1 (MQ=255)

             cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTGATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑g                                                                                    <  2:1683035/100‑1 (MQ=255)

                aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAg                                                                                 <  1:1022406/100‑1 (MQ=255)

                   gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGcc                                                                              <  1:2213964/100‑1 (MQ=255)

                   gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGcc                                                                              >  1:1525502/1‑100 (MQ=255)

                             tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCgg                                                                    <  1:225885/100‑1 (MQ=255)

                                 gaatttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑AT‑CCCTT‑‑GTAGGCCTGATAAGCGTAgcgc                                                               >  2:1092872/11‑100 (MQ=18)

                                 cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                                                <  2:2717074/100‑1 (MQ=255)

                                 cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                                                <  2:763909/100‑1 (MQ=255)

                                  attttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCA‑GA‑AA‑C‑CTT‑‑GTAGGCCTGATAAGCGTAgcgc                                                               >  1:1707567/10‑100 (MQ=17)

                                  attttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCATACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAgcgc                                                               >  2:2901299/10‑100 (MQ=18)

                                   ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCa                                                              <  1:1106731/100‑1 (MQ=255)

                                   atttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑ATCC‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCAt                                                             >  2:1615108/9‑100 (MQ=18)

                                    tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCA‑GG‑GA‑C‑TAT‑‑GTAGGCCTGATAAGCATAGCGCAt                                                             >  2:543247/8‑100 (MQ=18)

                                     gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                                                            >  2:457510/1‑100 (MQ=255)

                                      tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GC‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                                                           >  1:1220173/1‑100 (MQ=255)

                                      gatctGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                                                           <  1:731614/96‑1 (MQ=255)

                                       ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTACA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                                                          <  1:1546220/100‑1 (MQ=255)

                                       gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCA‑‑A‑TA‑T‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                         >  2:1299027/5‑100 (MQ=18)

                                       gtaggCCGGATAAGGCGTTAACGCCGCATCCGGCATACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                                                           >  2:2697775/5‑99 (MQ=17)

                                        taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCA‑GA‑GT‑T‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                         >  2:300596/4‑100 (MQ=17)

                                        taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCA‑GA‑GT‑T‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                         >  1:1718905/4‑100 (MQ=17)

                                        taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCA‑GT‑TT‑A‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAgg                                                         >  2:1307194/4‑100 (MQ=21)

                                        taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCA‑GAGAT‑T‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                         >  2:1911166/4‑100 (MQ=17)

                                         aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCA‑AT‑TC‑T‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                        >  2:2856373/3‑100 (MQ=18)

                                         aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAATCATGCAAATCCAAAATATTGCA‑GG‑AG‑T‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                       >  2:622935/3‑100 (MQ=21)

                                          tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                                                       >  2:22551/1‑100 (MQ=255)

                                          ggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCA‑GA‑AA‑T‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                       >  2:1770372/2‑100 (MQ=18)

                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑ATCC‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt                                                      >  2:2196595/2‑100 (MQ=18)

                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGG‑GC‑C‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                       >  2:2209020/2‑100 (MQ=17)

                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCA‑GAGAT‑T‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                       >  2:2580175/2‑100 (MQ=17)

                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAATCATGCAAATCCAAAATATTGCA‑GG‑AG‑T‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt                                                      >  2:2547557/2‑100 (MQ=21)

                                          ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                                                       >  2:1132295/2‑100 (MQ=17)

                                           gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                      >  2:819102/1‑100 (MQ=255)

                                           gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑ATCC‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                     >  1:1083590/1‑100 (MQ=18)

                                           gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCA‑TG‑GG‑C‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                      >  1:236359/1‑99 (MQ=17)

                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCA‑TG‑AG‑C‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                      >  1:1802046/1‑99 (MQ=17)

                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCA‑TG‑AG‑C‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                      >  1:1270798/1‑99 (MQ=17)

                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGAT‑CG‑AT‑C‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                      >  1:166973/1‑99 (MQ=21)

                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑‑A‑AT‑C‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                      >  1:2545427/1‑99 (MQ=18)

                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCA‑GG‑AT‑A‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                      >  1:785572/1‑99 (MQ=18)

                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAATCATGCAAATCCAAAATATTGCA‑GG‑AG‑T‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                     >  1:2454157/1‑100 (MQ=21)

                                            ccggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCA‑TG‑AG‑C‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                     >  2:2141641/9‑98 (MQ=17)

                                            ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGAT‑CG‑AT‑C‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                     >  1:1428842/9‑98 (MQ=21)

                                            ccggataaggcgTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGTGGAGCGCATCAGGCAtt                                                     >  2:1232612/9‑100 (MQ=18)

                                            ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                                                     >  1:2412885/1‑100 (MQ=255)

                                            ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                                                     >  2:2314444/1‑100 (MQ=255)

                                             cggataaggcgTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑ATCC‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttt                                                    >  2:2028565/8‑99 (MQ=18)

                                             cggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCA‑GG‑AG‑C‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagtt                                                    >  2:153092/8‑97 (MQ=18)

                                              ggataaggcgTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑ATCC‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                                                  >  2:1472089/7‑98 (MQ=21)

                                              ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCA‑GA‑GT‑T‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                   >  1:1648582/7‑100 (MQ=17)

                                              ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCA‑TG‑AG‑C‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                   >  1:2088474/7‑100 (MQ=17)

                                              ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCA‑CC‑AT‑C‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaattc                                                   >  2:1077259/7‑96 (MQ=21)

                                              ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCA‑GAGAT‑C‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                                                   >  2:2415926/7‑100 (MQ=17)

                                              ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAATCATGCAAATCCAAAATATTGCA‑GG‑AG‑T‑TAT‑‑GTAGGCCTGATAAGCGTAGTGCATCAGGCAtttat                                                  >  2:1862266/7‑98 (MQ=21)

                                               gataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCA‑GG‑AG‑C‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttg                                                  >  1:483435/6‑99 (MQ=18)

                                                ataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCA‑GA‑GT‑T‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttg                                                 <  2:382742/96‑1 (MQ=17)

                                                ataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCA‑GA‑GA‑T‑GGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCATTtttg                                                 <  1:383830/96‑1 (MQ=11)

                                                 tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                                                <  2:1420729/100‑1 (MQ=255)

                                                  gcggcgTTAACGCCTTATCCGGTCTACAAAATCGTGCAAATTCAATACAGTGCA‑GA‑AT‑T‑TTC‑‑GTAGGAATGATAAGCGGAGCGCATCAGGCATTtttgcc                                               <  2:2471634/100‑1 (MQ=18)

                                                  gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                                               <  2:2113156/100‑1 (MQ=255)

                                                   cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                                              >  1:1872371/1‑100 (MQ=255)

                                                   cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                                              >  2:373174/1‑100 (MQ=255)

                                                    ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt                                             >  1:2109630/1‑100 (MQ=255)

                                                     gcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgccttt                                            >  2:2102081/1‑100 (MQ=255)

                                                      cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg                                           <  1:825230/100‑1 (MQ=255)

                                                      cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg                                           <  1:2667617/100‑1 (MQ=255)

                                                       gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc                                          <  2:1665023/100‑1 (MQ=255)

                                                         tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa                                        >  1:373385/1‑100 (MQ=255)

                                                         tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa                                        >  1:683596/1‑100 (MQ=255)

                                                          aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt                                       >  1:1766420/1‑100 (MQ=255)

                                                             gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg                                    <  2:1594445/100‑1 (MQ=255)

                                                                 tATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCtt                                >  2:2358935/1‑100 (MQ=255)

                                                                  aTCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTg                               <  2:711334/100‑1 (MQ=255)

                                                                   tCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGt                              >  2:2697195/1‑100 (MQ=255)

                                                                    ccGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                             >  1:1473410/1‑100 (MQ=255)

                                                                     cGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTTTTTTTTTTTTCAGTCTTGTAt                            >  1:1883816/1‑100 (MQ=18)

                                                                         cTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCt                        >  1:436188/1‑100 (MQ=255)

                                                                         cTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCt                        >  2:1993701/1‑100 (MQ=255)

                                                                             aaaaTCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTa                    <  1:500813/100‑1 (MQ=255)

                                                                              aaaTCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTaa                   <  1:2901261/100‑1 (MQ=255)

                                                                               aaTCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAat                  <  1:593003/100‑1 (MQ=255)

                                                                                 tCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAatta                <  2:2610222/100‑1 (MQ=255)

                                                                                  cGTGCAAATTCAATACATTGCA‑GA‑AC‑T‑TTC‑‑GTAGGCCTGCTAAGCGGAGAGCATAAGGCATTTTTGCCTTTGCCATCAGCCTTGTATGTTTTTTAattat               <  1:963589/100‑1 (MQ=25)

                                                                                    tGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc             <  1:1132277/100‑1 (MQ=255)

                                                                                     gCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCg            >  2:887178/1‑100 (MQ=255)

                                                                                     gCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCg            <  1:1723240/100‑1 (MQ=255)

                                                                                      cAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGa           >  1:816311/1‑100 (MQ=255)

                                                                                      cAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGa           >  1:2311927/1‑100 (MQ=255)

                                                                                        aaTTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATa         >  2:642843/1‑100 (MQ=255)

                                                                                         aTTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATag        <  2:2085135/100‑1 (MQ=255)

                                                                                            cAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAgaga     <  1:1463185/100‑1 (MQ=255)

GCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGT  >  NC_000913/3705920‑3706114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`