Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,077,116 T→C E589E (GAA→GAG)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,077,1160TC95.7% 297.8 / 1.9 92E589E (GAA→GAG) putAfused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (35/53);  total (38/54)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

GCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGC  >  NC_000913/1077019‑1077212
                                                                                                 |                                                                                                
gCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTtctc                                                                                                <  2:2343797/100‑1 (MQ=255)
 cccTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTACCCTTCCTGTTGCGCCAGTTTctct                                                                                               <  2:316076/100‑1 (MQ=255)
  ccTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTa                                                                                              >  2:2579946/1‑100 (MQ=255)
   cTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAc                                                                                             >  1:1822963/1‑100 (MQ=255)
     gCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcag                                                                                           >  2:266251/1‑100 (MQ=255)
     gCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcag                                                                                           >  1:2111607/1‑100 (MQ=255)
     gCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcag                                                                                           >  2:266233/1‑100 (MQ=255)
      ccGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcagc                                                                                          <  2:280717/100‑1 (MQ=255)
       cGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTAcagca                                                                                         >  2:1562352/1‑100 (MQ=255)
        gAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcagcag                                                                                        >  2:137921/1‑100 (MQ=255)
          gTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTg                                                                                      <  2:1468230/100‑1 (MQ=255)
             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                   >  1:2028078/1‑100 (MQ=255)
             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                   <  1:813423/100‑1 (MQ=255)
                  gcCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATc                                                                              <  2:2759298/100‑1 (MQ=255)
                  gcCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATc                                                                              <  2:317829/100‑1 (MQ=255)
                  gcCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATc                                                                              <  2:2403176/100‑1 (MQ=255)
                  gcCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATc                                                                              <  2:2403004/100‑1 (MQ=255)
                     cGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGc                                                                           <  2:987464/100‑1 (MQ=255)
                     cGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGc                                                                           >  2:1983838/1‑100 (MQ=255)
                      gTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCg                                                                          <  1:1442325/100‑1 (MQ=255)
                       tGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGa                                                                         >  2:1286662/1‑100 (MQ=255)
                        gACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAc                                                                        >  2:1511337/1‑100 (MQ=255)
                        gACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAc                                                                        <  1:2706443/100‑1 (MQ=255)
                        gACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAc                                                                        <  1:787777/100‑1 (MQ=255)
                         aCCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAcc                                                                       <  1:2182753/100‑1 (MQ=255)
                         aCCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAcc                                                                       <  1:2400828/100‑1 (MQ=255)
                         aCCGTAAAGATCGCGCGGCAGGGGAATTGTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAcc                                                                       <  2:1979784/100‑1 (MQ=255)
                          ccGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAcc                                                                       >  2:483831/1‑99 (MQ=255)
                          ccGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCa                                                                      >  2:606676/1‑100 (MQ=255)
                          ccGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCa                                                                      <  1:1169204/100‑1 (MQ=255)
                           cGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAg                                                                     <  2:113891/100‑1 (MQ=255)
                             tAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGtn                                                                   <  2:1667186/100‑2 (MQ=255)
                               ttccgatctcgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCa                                                                 <  1:34040/91‑1 (MQ=255)
                                aGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCAt                                                                >  2:725540/1‑100 (MQ=255)
                                 gATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATc                                                               <  1:1409175/100‑1 (MQ=255)
                                 gATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATc                                                               >  1:18934/1‑100 (MQ=255)
                                 gATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATc                                                               >  2:1609790/1‑100 (MQ=255)
                                 gATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATc                                                               <  1:2011898/100‑1 (MQ=255)
                                  aTCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATcc                                                              <  2:613576/100‑1 (MQ=255)
                                      cgcgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTg                                                          <  1:543590/100‑1 (MQ=255)
                                        cgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCaagatcggaa                                                        >  2:34041/1‑91 (MQ=255)
                                         ggCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCATTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGATCATCCAGTGGc                                                        >  1:229250/1‑99 (MQ=255)
                                            aGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAag                                                    >  2:1475754/1‑100 (MQ=255)
                                             ggggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAaga                                                   <  1:2062271/100‑1 (MQ=255)
                                              gggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAagag                                                  <  1:528013/100‑1 (MQ=255)
                                              gggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAagag                                                  >  1:462475/1‑100 (MQ=255)
                                               ggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                                 <  1:1232929/100‑1 (MQ=255)
                                                   ttttCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTc                                             >  2:890559/1‑100 (MQ=255)
                                                   ttttCGGATGCGGTAATCCAGTTTGCCCTTCAAGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTc                                             >  1:2684253/1‑100 (MQ=255)
                                                    tttCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCg                                            <  2:416378/100‑1 (MQ=255)
                                                    tttCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCg                                            <  2:1168757/100‑1 (MQ=255)
                                                     ttCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCgg                                           <  2:2384504/100‑1 (MQ=255)
                                                     ttCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGGATCTCTACAGCAGTGACCGGATCAGCGACAAGTTCGTCGAGTGGCAAAGAGgtgt                                              >  1:2665749/1‑97 (MQ=255)
                                                      tCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGTAAAGAGGTGTCggn                                          <  1:1202447/100‑2 (MQ=255)
                                                      tCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGc                                          <  2:712847/100‑1 (MQ=255)
                                                      tCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGc                                          <  2:2028106/100‑1 (MQ=255)
                                                        ggATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCaa                                        >  1:2898284/1‑100 (MQ=255)
                                                           tGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATAc                                     >  2:2150738/1‑100 (MQ=255)
                                                             cGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACgg                                   <  1:1822088/100‑1 (MQ=255)
                                                             cGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACgg                                   >  2:1329266/1‑100 (MQ=255)
                                                              ggTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGt                                  <  2:995495/100‑1 (MQ=255)
                                                               gTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGtt                                 <  1:2381085/100‑1 (MQ=255)
                                                                  aTCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAAc                              <  1:379266/100‑1 (MQ=255)
                                                                  aTCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAAc                              >  1:951703/1‑100 (MQ=255)
                                                                   tCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACa                             <  1:2427440/100‑1 (MQ=255)
                                                                    ccAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACaa                            >  1:247669/1‑100 (MQ=255)
                                                                    ccAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACaa                            >  2:820457/1‑100 (MQ=255)
                                                                      aGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAAc                          >  2:1090826/1‑100 (MQ=255)
                                                                         ttGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAg                       <  2:660216/100‑1 (MQ=255)
                                                                         ttGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAg                       <  2:1401353/100‑1 (MQ=255)
                                                                          tGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAgg                      <  1:750412/100‑1 (MQ=255)
                                                                          tGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAgg                      <  2:1283308/100‑1 (MQ=255)
                                                                          tGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAgg                      <  1:1090810/100‑1 (MQ=255)
                                                                            cccTTCCTGTGGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgtg                    <  2:1316543/100‑1 (MQ=255)
                                                                             ccTTCCTGTTGCGCCCGTTTCTCTACAGCAGTGACCGGAGCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgtgt                   <  1:483826/100‑1 (MQ=255)
                                                                              cTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGtt                  <  1:890550/100‑1 (MQ=255)
                                                                                tCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAg                <  1:1475730/100‑1 (MQ=255)
                                                                                tCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAg                <  2:749249/100‑1 (MQ=255)
                                                                                tCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAg                <  2:2111635/100‑1 (MQ=255)
                                                                                 ccTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGc               <  2:1822989/100‑1 (MQ=255)
                                                                                  cTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCa              >  2:1945006/1‑100 (MQ=255)
                                                                                  cTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCa              <  2:229253/100‑1 (MQ=255)
                                                                                   tGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAc             >  1:1420382/1‑100 (MQ=255)
                                                                                   tGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAc             >  1:1419266/1‑100 (MQ=255)
                                                                                   tGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAc             >  2:437021/1‑100 (MQ=255)
                                                                                    gTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGcccc            <  1:2579913/100‑1 (MQ=255)
                                                                                     ttGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCg           <  2:2397338/100‑1 (MQ=255)
                                                                                       gcgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGtt         >  2:388408/1‑100 (MQ=255)
                                                                                        cgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGttt        >  1:1651690/1‑100 (MQ=255)
                                                                                         gcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGtttt       >  2:1468195/1‑100 (MQ=255)
                                                                                           cAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTcc     <  1:254751/100‑1 (MQ=255)
                                                                                           cAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTcc     <  2:2321354/100‑1 (MQ=255)
                                                                                           cAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTcc     <  2:1857936/100‑1 (MQ=255)
                                                                                            aGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCca    >  2:1662740/1‑100 (MQ=255)
                                                                                              tttctCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCcagc  >  2:1793659/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGC  >  NC_000913/1077019‑1077212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑