BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,997	G→A	T68T (ACG→ACA)	yceD →	DUF177 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,997	0	G	A	99.2%	440.3 / ‑4.6	128	T68T (ACG→ACA)	yceD	DUF177 family protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (63/63); total (63/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 128 total aligned reads displayed.

ATCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAA  >  NC_000913/1146901‑1147093

aTCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTa                                                                                               <  2:1220856/100‑1 (MQ=255)

 tCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTaa                                                                                              <  2:2320021/100‑1 (MQ=255)

  ccGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAAc                                                                                             >  1:1656368/1‑100 (MQ=255)

  ccGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAAc                                                                                             <  2:1006190/100‑1 (MQ=255)

  ccGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAAc                                                                                             <  2:2832617/100‑1 (MQ=255)

   cGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACg                                                                                            >  2:2630888/1‑100 (MQ=255)

   cGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACg                                                                                            <  1:916119/100‑1 (MQ=255)

   cGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACg                                                                                            >  1:2176537/1‑100 (MQ=255)

       gTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCg                                                                                        <  1:735293/100‑1 (MQ=255)

       gTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCg                                                                                        >  2:204909/1‑100 (MQ=255)

       gTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCg                                                                                        >  1:851575/1‑100 (MQ=255)

         cAGTGTGGACAGTGATGTGGAATGCTTCATGTCGTTCGCTATCGATAAACAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCg                                                                                        <  2:502714/98‑1 (MQ=255)

         cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                      <  2:1085031/100‑1 (MQ=255)

         cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                      <  2:848943/100‑1 (MQ=255)

         cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                      <  1:1006405/100‑1 (MQ=255)

          aGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgt                                                                                     >  2:165052/1‑100 (MQ=255)

          aGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                      >  1:396841/1‑99 (MQ=255)

            tgtgGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgtgt                                                                                   >  1:2343890/1‑100 (MQ=255)

             gtgGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgtgt                                                                                   >  1:1252760/1‑99 (MQ=255)

              tgGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCa                                                                                 <  2:2655504/100‑1 (MQ=255)

               ggACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAg                                                                                >  1:848843/1‑100 (MQ=255)

                  cAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTGACGCTCGAGTGTCAGCGt                                                                             >  2:2304348/1‑100 (MQ=255)

                  cAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGt                                                                             >  2:90901/1‑100 (MQ=255)

                    gTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTg                                                                           >  2:1322718/1‑100 (MQ=255)

                     tGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGc                                                                          <  2:1072122/100‑1 (MQ=255)

                      gATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCg                                                                         <  1:1299398/100‑1 (MQ=255)

                      gATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCg                                                                         <  2:2362684/100‑1 (MQ=255)

                      gATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCg                                                                         >  2:1924127/1‑100 (MQ=255)

                       aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCgg                                                                        <  2:352348/100‑1 (MQ=255)

                          tgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGaa                                                                     >  2:342273/1‑100 (MQ=255)

                           ggAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAg                                                                    <  2:814813/100‑1 (MQ=255)

                              aTGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCg                                                                 <  1:2062477/100‑1 (MQ=255)

                                gCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                               <  2:2905552/100‑1 (MQ=255)

                                 cTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGttt                                                              <  1:1040082/100‑1 (MQ=255)

                                  tCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTa                                                             >  2:928382/1‑100 (MQ=255)

                                   ccATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTAc                                                            <  1:338288/100‑1 (MQ=255)

                                   ccATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTAc                                                            <  1:1449592/100‑1 (MQ=255)

                                    cATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACt                                                           <  2:1732517/100‑1 (MQ=255)

                                     aTGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGTCGTTTACtc                                                          <  2:2167834/100‑1 (MQ=255)

                                      tGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtca                                                         >  1:1434182/1‑100 (MQ=255)

                                      tGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTAAGCGTTGCGGGAAGCCGTTTACtca                                                         >  2:2746622/1‑100 (MQ=255)

                                        tCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcatn                                                       <  2:1562527/100‑2 (MQ=255)

                                           ttCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAgg                                                    <  2:1595810/100‑1 (MQ=255)

                                           ttCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAgg                                                    <  1:326471/100‑1 (MQ=255)

                                            tCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGt                                                   >  2:2801474/1‑100 (MQ=255)

                                             cGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTc                                                  <  2:720138/100‑1 (MQ=255)

                                             cGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTc                                                  <  2:2564587/100‑1 (MQ=255)

                                             cGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTc                                                  <  1:2022804/100‑1 (MQ=255)

                                             cGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTc                                                  <  1:954518/100‑1 (MQ=255)

                                             cGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTc                                                  >  1:2585451/1‑100 (MQ=255)

                                              gCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCt                                                 >  1:175156/1‑100 (MQ=255)

                                              gCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCt                                                 <  2:851581/100‑1 (MQ=255)

                                              gCTATCGATAACCAACGGCTCGCAGTTTTAAACGGCGATGCGAAGGTGACCGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTc                                                  <  1:1248206/99‑1 (MQ=255)

                                               cTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGATCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCAACAGGTCTa                                                >  2:2715053/1‑100 (MQ=255)

                                                 aTCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTaca                                              <  2:2359131/100‑1 (MQ=255)

                                                  tCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTacac                                             >  1:1941189/1‑100 (MQ=255)

                                                  tCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTacac                                             >  2:806077/1‑100 (MQ=255)

                                                   cGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTacac                                             >  1:723783/1‑99 (MQ=255)

                                                    gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACaa                                           <  2:2731647/100‑1 (MQ=255)

                                                    gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACaa                                           >  1:2023776/1‑100 (MQ=255)

                                                    gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACaa                                           >  2:1451029/1‑100 (MQ=255)

                                                    gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACaa                                           >  2:1192225/1‑100 (MQ=255)

                                                      tAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACg                                         <  1:1348029/100‑1 (MQ=255)

                                                       aaCCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGt                                        >  1:269922/1‑100 (MQ=255)

                                                         ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      >  2:2307935/1‑100 (MQ=255)

                                                         ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      >  1:1051126/1‑100 (MQ=255)

                                                         ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      >  2:1870948/1‑100 (MQ=255)

                                                               tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                <  2:2296930/100‑1 (MQ=255)

                                                                 tcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAg                              <  2:2166239/100‑1 (MQ=255)

                                                                 tcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAg                              <  1:2461671/100‑1 (MQ=255)

                                                                  cGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGt                             >  2:1995947/1‑100 (MQ=255)

                                                                  cGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGt                             <  1:1784731/100‑1 (MQ=255)

                                                                   gCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTc                            <  2:1656394/100‑1 (MQ=255)

                                                                    cAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTcc                           <  1:1647999/100‑1 (MQ=255)

                                                                    cAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTcc                           >  1:814269/1‑100 (MQ=255)

                                                                    cAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTcc                           >  2:1492441/1‑100 (MQ=255)

                                                                    cAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTcc                           <  1:90899/100‑1 (MQ=255)

                                                                    cAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTcc                           <  1:165050/100‑1 (MQ=255)

                                                                        gtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTTACCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCtgcg                       <  1:2041315/100‑3 (MQ=255)

                                                                        gtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCtgtg                       <  2:1849496/100‑1 (MQ=255)

                                                                        gtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTGTAGTCCtgtg                       >  1:533787/1‑100 (MQ=255)

                                                                          tAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCg                     <  1:1122045/100‑1 (MQ=255)

                                                                          tAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCg                     >  2:2482470/1‑100 (MQ=255)

                                                                            aaCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGtt                   >  2:451488/1‑100 (MQ=255)

                                                                            aaCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGtt                   >  1:2342783/1‑100 (MQ=255)

                                                                             aCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTc                  <  1:1255834/100‑1 (MQ=255)

                                                                              cGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCa                 >  2:83644/1‑100 (MQ=255)

                                                                                gCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGa               <  1:2304321/100‑1 (MQ=255)

                                                                                 cGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGAc              >  1:929689/1‑100 (MQ=255)

                                                                                 cGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTCCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGAc              <  1:1583391/100‑1 (MQ=255)

                                                                                  gATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACg             <  2:486711/100‑1 (MQ=255)

                                                                                   aTGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGa            >  1:2757270/1‑100 (MQ=255)

                                                                                    tGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAAGGTATTGTTATAGTCCTGTGCGTTCAGACGaa           >  1:1958865/1‑100 (MQ=255)

                                                                                    tGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGaa           >  1:1716285/1‑100 (MQ=255)

                                                                                    tGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGaa           >  1:649179/1‑100 (MQ=255)

                                                                                     gCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAAc          <  2:2873866/100‑1 (MQ=255)

                                                                                       gAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAg        >  1:2892466/1‑100 (MQ=255)

                                                                                         aGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGc      <  2:2549230/100‑1 (MQ=255)

                                                                                         aGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGc      <  2:2549206/100‑1 (MQ=255)

                                                                                          ggTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCt     <  2:1239474/100‑1 (MQ=255)

ATCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAA  >  NC_000913/1146901‑1147093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`