BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	91,816	G→A	R135H (CGC→CAC)	ftsI →	transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	91,816	0	G	A	100.0%	375.3 / NA	108	R135H (CGC→CAC)	ftsI	transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (59/49); total (59/49)

Only 100 of 108 total aligned reads displayed.

GTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCC  >  NC_000913/91720‑91911

gTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCAGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtt                                                                                              >  1:1103139/1‑100 (MQ=255)

   tCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttat                                                                                           >  2:499967/1‑100 (MQ=255)

    cAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttatt                                                                                          >  1:1063779/1‑100 (MQ=255)

    cAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttatt                                                                                          <  2:198515/100‑1 (MQ=255)

    cAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttatt                                                                                          <  2:1862540/100‑1 (MQ=255)

    cAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttatt                                                                                          <  2:1242243/100‑1 (MQ=255)

     aGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttattt                                                                                         >  2:118593/1‑100 (MQ=255)

     aGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttattt                                                                                         <  1:987538/100‑1 (MQ=255)

     aGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttattt                                                                                         >  1:634205/1‑100 (MQ=255)

      gCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACtttattta                                                                                        <  2:92302/100‑1 (MQ=255)

       cGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTttatttat                                                                                       <  1:1657595/100‑1 (MQ=255)

          cGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCCTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTg                                                                                    <  2:2608854/100‑1 (MQ=255)

          cGGTGACCGCTGGAAGGCGCGGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTg                                                                                    >  1:1768924/1‑100 (MQ=255)

           ggTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTgg                                                                                   >  2:1819518/1‑100 (MQ=255)

           ggTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTgg                                                                                   <  1:2391522/100‑1 (MQ=255)

           ggTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTgg                                                                                   >  1:2297428/1‑100 (MQ=255)

           ggTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTgg                                                                                   >  2:109590/1‑100 (MQ=255)

            gTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGgc                                                                                  <  2:1616007/100‑1 (MQ=255)

            gTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGgc                                                                                  <  2:757728/100‑1 (MQ=255)

            gTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGgc                                                                                  <  1:1285659/100‑1 (MQ=255)

              gACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGgcgc                                                                                >  1:1795177/1‑100 (MQ=255)

              gACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGgcgc                                                                                >  1:637699/1‑100 (MQ=255)

              gACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGgcgc                                                                                >  1:722673/1‑100 (MQ=255)

               aCCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGcgcg                                                                               <  1:1725352/100‑1 (MQ=255)

               aCCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGcgcg                                                                               <  1:823010/100‑1 (MQ=255)

                ccGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGgcgc                                                                                >  2:1282520/1‑98 (MQ=255)

                ccGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGt                                                                              >  1:2256326/1‑100 (MQ=255)

                ccGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGt                                                                              >  2:16921/1‑100 (MQ=255)

                  gCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCa                                                                            >  1:2399353/1‑100 (MQ=255)

                   cTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAg                                                                           <  1:2543562/100‑1 (MQ=255)

                   cTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAg                                                                           <  2:1430384/100‑1 (MQ=255)

                     ggAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGt                                                                         <  1:75992/100‑1 (MQ=255)

                     ggAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGt                                                                         >  2:22813/1‑100 (MQ=255)

                     ggAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGt                                                                         >  2:2293834/1‑100 (MQ=255)

                     ggAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGt                                                                         >  2:2759442/1‑100 (MQ=255)

                      gAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTg                                                                        >  2:1743159/1‑100 (MQ=255)

                      gAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTg                                                                        <  1:1298698/100‑1 (MQ=255)

                          gcgcTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAAcc                                                                    <  1:1136229/100‑1 (MQ=255)

                          gcgcTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAAcc                                                                    <  1:1224774/100‑1 (MQ=255)

                           cgcTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAAccc                                                                   >  1:2018524/1‑100 (MQ=255)

                            gcTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCt                                                                  >  1:2176483/1‑100 (MQ=255)

                            gcTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCAATTTATTTATCTGGCGCGTCAGGTGAAACCt                                                                  >  2:532805/1‑100 (MQ=255)

                             cTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTg                                                                 <  1:1380887/100‑1 (MQ=255)

                             cTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTg                                                                 >  2:421569/1‑100 (MQ=255)

                              tGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAgggttcacctga                                                                <  2:742114/100‑11 (MQ=255)

                               ggCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGAc                                                               <  2:1561525/100‑1 (MQ=255)

                               ggCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGAc                                                               <  2:1181699/100‑1 (MQ=255)

                                 cTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACAt                                                             >  2:1310479/1‑100 (MQ=255)

                                    aCGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGc                                                          <  2:1575279/100‑1 (MQ=255)

                                      gcgcTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCgg                                                        <  1:1758182/100‑1 (MQ=255)

                                        gcTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGAc                                                      >  1:1845800/1‑100 (MQ=255)

                                        gcTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGAc                                                      >  2:1593920/1‑100 (MQ=255)

                                        gcTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGAc                                                      >  2:142502/1‑100 (MQ=255)

                                           cAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGTCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTTAACCCTGACATGGCGGACTAc                                                   >  1:2499548/1‑100 (MQ=255)

                                           cAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTAc                                                   <  2:1698990/100‑1 (MQ=255)

                                           cAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTAc                                                   <  1:1262308/100‑1 (MQ=255)

                                           cAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTAc                                                   >  2:2700532/1‑100 (MQ=255)

                                            aaTATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACa                                                  >  1:1876924/1‑100 (MQ=255)

                                                  ccGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCaaaa                                            >  2:234291/1‑100 (MQ=255)

                                                  ccGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCaaaa                                            >  2:480819/1‑100 (MQ=255)

                                                  ccGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCaa                                              >  1:2482123/1‑98 (MQ=255)

                                                   cGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCaaaaa                                           >  2:1162615/1‑100 (MQ=255)

                                                   cGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGAATACATCaaaaa                                           <  1:530695/100‑1 (MQ=255)

                                                     cTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaac                                         <  2:2060050/100‑1 (MQ=255)

                                                       ggATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaactg                                       >  1:1413876/1‑100 (MQ=255)

                                                       ggATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaactg                                       >  2:2236586/1‑100 (MQ=255)

                                                       ggATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaact                                        >  2:1052541/1‑99 (MQ=255)

                                                         ntCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGTGGACTACATCAAAaaactgaa                                     >  1:2389495/2‑100 (MQ=255)

                                                         aTCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGAGATGGCGGACTACATCAAAaaactgaa                                     <  2:1770535/100‑1 (MQ=255)

                                                         aTCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaactgaa                                     >  2:505559/1‑100 (MQ=255)

                                                         aTCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaactgaa                                     >  2:126885/1‑100 (MQ=255)

                                                           cAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaactgaaac                                   <  1:2572581/100‑1 (MQ=255)

                                                           cAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaactgaaac                                   <  2:2202075/100‑1 (MQ=255)

                                                            aGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAaaactgaaact                                  >  1:2758497/1‑100 (MQ=255)

                                                              ntttCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGc                                >  1:53867/2‑100 (MQ=255)

                                                                ttCAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCg                              >  2:1531406/1‑100 (MQ=255)

                                                                  cAGCCCGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCggg                            >  2:920799/1‑100 (MQ=255)

                                                                     cccGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGAt                         <  1:1466771/100‑1 (MQ=255)

                                                                      ccGCATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGAtt                        >  2:1656456/1‑100 (MQ=255)

                                                                         cATTAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCAt                     >  1:1766593/1‑100 (MQ=255)

                                                                           ttAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCt                   >  2:859264/1‑100 (MQ=255)

                                                                            tAACGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTg                  >  2:1135296/1‑100 (MQ=255)

                                                                             aaCGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGc                 >  1:1314099/1‑100 (MQ=255)

                                                                             aaCGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGc                 >  2:2890969/1‑100 (MQ=255)

                                                                              aCGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCg                <  2:2176510/100‑1 (MQ=255)

                                                                               cGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGt               >  2:228537/1‑100 (MQ=255)

                                                                               cGCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGt               >  1:177427/1‑100 (MQ=255)

                                                                                gCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTg              <  2:2300884/100‑1 (MQ=255)

                                                                                gCCAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTg              >  1:2805970/1‑100 (MQ=255)

                                                                                 ncAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGa             >  2:2702776/2‑100 (MQ=255)

                                                                                 ccAACCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGa             <  2:1795874/100‑1 (MQ=255)

                                                                                   aaCCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAag           <  1:1027977/100‑1 (MQ=255)

                                                                                   aaCCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAag           <  1:822696/100‑1 (MQ=255)

                                                                                    aCCCGAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAaga          <  1:432160/100‑1 (MQ=255)

                                                                                     cccGAAAGGGCACTTGATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAagag         <  2:634211/100‑1 (MQ=255)

                                                                                        gAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGtct      <  2:1333069/100‑1 (MQ=255)

                                                                                        gAAAGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACAACAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGtct      <  1:109588/100‑1 (MQ=255)

                                                                                         aaaGGGCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGtctc     <  1:2077761/100‑1 (MQ=255)

                                                                                            gggCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGcc  <  1:16920/100‑1 (MQ=255)

                                                                                            gggCACTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGcc  <  1:234287/100‑1 (MQ=255)

GTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCC  >  NC_000913/91720‑91911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`