Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,554,244	G→A	D313D (GAC→GAT)	eutR ←	eut operon transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,554,244	0	G	A	100.0%	252.6 / NA	73	D313D (GAC→GAT)	eutR	eut operon transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/39);  total (34/39)

CCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCT  >  NC_000913/2554148‑2554340                                                                                                 |                                                                                                 ccGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCt                                                                                               <  1:514360/100‑1 (MQ=255)  cGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCtt                                                                                              >  2:1209532/1‑100 (MQ=255)  cGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCtt                                                                                              <  2:1620092/100‑1 (MQ=255)    cTGATGCAGCGTCTGTGACGGCTTCTCGGAAAACAGCTGCTGATAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCtttt                                                                                            >  2:1964552/1‑100 (MQ=255)    cTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCtttt                                                                                            >  2:915552/1‑100 (MQ=255)        tGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCg                                                                                        <  2:246796/100‑1 (MQ=255)        tGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCg                                                                                        >  1:684355/1‑100 (MQ=255)        tGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCg                                                                                        >  1:1711751/1‑100 (MQ=255)         gaaGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCg                                                                                        >  1:388316/3‑99 (MQ=255)          cAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTc                                                                                      >  1:746166/1‑100 (MQ=255)                  gTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTg                                                                              >  1:2275763/1‑100 (MQ=255)                   tGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCGGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGc                                                                             <  2:177757/100‑1 (MQ=255)                   tGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGc                                                                             >  1:81901/1‑100 (MQ=255)                   tGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGc                                                                             <  2:1444123/100‑1 (MQ=255)                   tGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGc                                                                             <  2:865814/100‑1 (MQ=255)                    gACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCg                                                                            <  2:684361/100‑1 (MQ=255)                    gACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCg                                                                            <  2:81903/100‑1 (MQ=255)                    gACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCg                                                                            >  2:1817459/1‑100 (MQ=255)                     aCGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGa                                                                           <  2:1145804/100‑1 (MQ=255)                         cTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCAc                                                                       >  1:2510080/1‑100 (MQ=255)                             tcGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGAc                                                                   <  1:2556851/100‑1 (MQ=255)                             tcGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGAc                                                                   <  2:1435827/100‑1 (MQ=255)                             tcGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGAc                                                                   <  2:1436182/100‑1 (MQ=255)                             tcGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGAc                                                                   <  1:2377842/100‑1 (MQ=255)                              cGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACt                                                                  <  1:2077760/100‑1 (MQ=255)                              cGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACt                                                                  <  2:1594923/100‑1 (MQ=255)                              cGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACt                                                                  <  2:58242/100‑1 (MQ=255)                                    aCAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAg                                                            <  1:452666/100‑1 (MQ=255)                                       gctgctGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTc                                                         >  1:130770/1‑100 (MQ=255)                                         tgctgGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTcgc                                                       >  1:1255927/1‑100 (MQ=255)                                          gctgGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTcgcg                                                      >  1:1781938/1‑100 (MQ=255)                                          gctgGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTcgcg                                                      <  2:1151775/100‑1 (MQ=255)                                          gctgGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTcgcg                                                      <  2:2528843/100‑1 (MQ=255)                                           ctgGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCgcgg                                                     >  1:2875526/1‑100 (MQ=255)                                                 aTCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACg                                               >  2:2151919/1‑100 (MQ=255)                                                    cGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCg                                            >  2:108988/1‑100 (MQ=255)                                                    cGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCg                                            <  2:1804820/100‑1 (MQ=255)                                                     gTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGTGACCACGGACTTATCAGTTCGCGGCGTACGGCGt                                           >  1:646145/1‑100 (MQ=255)                                                     gTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGt                                           <  1:915543/100‑1 (MQ=255)                                                       ggCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTc                                         >  2:2135762/1‑100 (MQ=255)                                                       ggCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTc                                         >  1:2005086/1‑100 (MQ=255)                                                        gCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCa                                        <  2:388321/100‑1 (MQ=255)                                                         cAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAg                                       >  2:1171251/1‑100 (MQ=255)                                                         cAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAg                                       >  1:560108/1‑100 (MQ=255)                                                          aaaTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAgg                                      <  1:649194/100‑1 (MQ=255)                                                          aaaTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAgg                                      <  2:2027145/100‑1 (MQ=255)                                                           aaTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGc                                     >  2:120795/1‑100 (MQ=255)                                                           aaTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGc                                     >  2:1950365/1‑100 (MQ=255)                                                            aTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCg                                    <  2:1491644/100‑1 (MQ=255)                                                               gCCCCAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAAt                                 >  2:2562005/1‑100 (MQ=255)                                                                ccccAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATg                                >  2:1247011/1‑100 (MQ=255)                                                                 cccAGATGCCAGAATCCCCTCTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGc                               <  2:2703840/100‑1 (MQ=255)                                                                 cccAGATGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGc                               >  1:487637/1‑100 (MQ=255)                                                                       tGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCa                         <  2:721640/100‑1 (MQ=255)                                                                       tGCCAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCa                         <  1:1209514/100‑1 (MQ=255)                                                                         ccAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGc                       >  1:1359997/1‑100 (MQ=255)                                                                          cAGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGcc                      >  2:2382394/1‑100 (MQ=255)                                                                           aGAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCa                     >  1:2778940/1‑100 (MQ=255)                                                                            gAATCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCAc                    <  2:538943/100‑1 (MQ=255)                                                                              aTCCCCACTGCATGGCGGCATCTTTTACCGTCATACTTGGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAACCAcgc                  <  1:1444229/100‑1 (MQ=255)                                                                                ccccACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGt                >  2:2858523/1‑100 (MQ=255)                                                                                 nccACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCAcgcgct               >  2:2459276/2‑98 (MQ=255)                                                                                 cccACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGtt               <  2:2029228/100‑1 (MQ=255)                                                                                   cACTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGGCTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCg             <  2:1410041/100‑1 (MQ=255)                                                                                    aCTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCgg            <  2:1218955/100‑1 (MQ=255)                                                                                     cTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCggg           <  2:463833/100‑1 (MQ=255)                                                                                     cTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCggg           <  1:120140/100‑1 (MQ=255)                                                                                     cTGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCggg           <  2:2191156/100‑1 (MQ=255)                                                                                      tGCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGc          <  2:1719919/100‑1 (MQ=255)                                                                                       gCATGGCGGCATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGcc         <  2:891124/100‑1 (MQ=255)                                                                                           ggcggcATCTTTTACCGTCATACTTTGCGACCATGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGACTGCGTTTCAGCCACGCGTTCGGGCCAATg     >  1:1748227/1‑100 (MQ=255)                                                                                           ggcggcATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATg     <  1:1950337/100‑1 (MQ=255)                                                                                           ggcggcATCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATg     >  1:2368537/1‑100 (MQ=255)                                                                                            gcggcaTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGc    >  2:753307/1‑100 (MQ=255)                                                                                              ggcaTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCt  >  1:2236/1‑100 (MQ=255)                                                                                              ggcaTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCt  >  2:636106/1‑100 (MQ=255)                                                                                              ggcaTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCt  <  1:1574232/100‑1 (MQ=255)                                                                                                 |                                                                                                 CCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCT  >  NC_000913/2554148‑2554340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑