Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,086,935 G→T G77V (GGC→GTC)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,086,9350GT96.2% 351.5 / 2.0 105G77V (GGC→GTC) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (42/59);  total (44/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

ACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAGGGCGTTGACCG  >  NC_000913/3086839‑3087031
                                                                                                |                                                                                                
aCGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTa                                                                                               <  1:1476731/100‑1 (MQ=255)
 cGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTAt                                                                                              >  1:1571699/1‑100 (MQ=255)
   tAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACAACCAGCGCCTGGGTAGACATCGAAGAGATGACCCGTAACACCGTTCGCGAAATTGTGTAtgt                                                                                            >  1:2416459/1‑100 (MQ=255)
    aaaaaCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTAtgtg                                                                                           <  2:606175/100‑1 (MQ=255)
    aaaaaCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTAtgtg                                                                                           <  1:1874210/100‑1 (MQ=255)
    aaaaaCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTAtgtg                                                                                           <  2:101723/100‑1 (MQ=255)
    aaaaaCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAAGTGTCTAtgtg                                                                                           >  2:2315862/1‑100 (MQ=255)
     aaaaCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGc                                                                                          >  1:2265117/1‑100 (MQ=255)
      aaaCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCa                                                                                         <  2:2209921/100‑1 (MQ=255)
        aCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCAtt                                                                                       <  1:552277/100‑1 (MQ=255)
        aCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCAtt                                                                                       <  1:31059/100‑1 (MQ=255)
          cGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTcc                                                                                     >  2:1876221/1‑100 (MQ=255)
          cGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTcc                                                                                     <  2:596590/100‑1 (MQ=255)
           ggCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTc                                                                                      >  1:1166274/1‑98 (MQ=255)
           ggCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCg                                                                                    <  2:1982604/100‑1 (MQ=255)
            gCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGa                                                                                   >  2:1053372/1‑100 (MQ=255)
             cATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGAc                                                                                  <  2:2634169/100‑1 (MQ=255)
                  ttttAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATggg                                                                             <  2:2460501/100‑1 (MQ=255)
                  ttttAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATggg                                                                             <  2:595841/100‑1 (MQ=255)
                  ttttAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATggg                                                                             <  1:2627224/100‑1 (MQ=255)
                  ttttAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATggg                                                                             >  1:1069712/1‑100 (MQ=255)
                   tttAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGc                                                                            >  1:2382933/1‑100 (MQ=255)
                    ctAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCt                                                                           <  2:2009528/99‑1 (MQ=255)
                     tAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCtt                                                                          >  2:1523806/1‑100 (MQ=255)
                     tAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCtt                                                                          <  2:1396832/100‑1 (MQ=255)
                      aGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCttt                                                                         >  2:1433308/1‑100 (MQ=255)
                      aGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCttt                                                                         <  1:1101049/100‑1 (MQ=255)
                      aGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCtag                                                                         >  1:2009500/1‑98 (MQ=255)
                       gTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTg                                                                        <  1:2877721/100‑1 (MQ=255)
                       gTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTg                                                                        >  1:1479879/1‑100 (MQ=255)
                       gTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACAACGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTg                                                                        >  1:1549231/1‑100 (MQ=255)
                         tGGCGTAGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCTCCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGAc                                                                      <  1:1023920/100‑1 (MQ=255)
                         tGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCACGAAATTGTCTATGTGCATTCCGACATGGGCTTTGAc                                                                      >  1:2456092/1‑100 (MQ=255)
                          ggcggcGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACg                                                                     >  1:1447189/1‑100 (MQ=255)
                          ggcggcGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACg                                                                     <  2:1084054/100‑1 (MQ=255)
                           gcggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGc                                                                    >  2:545353/1‑100 (MQ=255)
                           gcggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGc                                                                    <  1:1876195/100‑1 (MQ=255)
                            cggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCt                                                                   <  1:424064/100‑1 (MQ=255)
                            cggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCt                                                                   >  1:1645167/1‑100 (MQ=255)
                            cggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCt                                                                   <  2:893308/100‑1 (MQ=255)
                             ggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTa                                                                  <  2:2201075/100‑1 (MQ=255)
                             ggcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTa                                                                  <  1:2750392/100‑1 (MQ=255)
                              gcgAAATCACCACCAGCGCCTGGGTAGACATCGATGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTa                                                                  <  2:225994/99‑1 (MQ=255)
                              gcgAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTaa                                                                 >  1:2120470/1‑100 (MQ=255)
                                gAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACt                                                               >  2:378853/1‑100 (MQ=255)
                                gAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACt                                                               >  2:378414/1‑100 (MQ=255)
                                  aaTCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTcc                                                             >  1:674537/1‑100 (MQ=255)
                                  aaTCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTcc                                                             <  2:2115000/100‑1 (MQ=255)
                                    tCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCtg                                                           <  2:103925/100‑1 (MQ=255)
                                    tCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCtg                                                           >  1:882960/1‑100 (MQ=255)
                                     caccacCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCtgt                                                          >  2:2683171/1‑100 (MQ=255)
                                       ccaccaGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGc                                                        >  2:1203012/1‑100 (MQ=255)
                                       ccaccaGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGc                                                        <  2:189727/100‑1 (MQ=255)
                                          ccaGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCgg                                                      >  1:700580/1‑99 (MQ=255)
                                            aGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc                                                   <  1:1597169/100‑1 (MQ=255)
                                            aGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc                                                   <  2:1532365/100‑1 (MQ=255)
                                            aGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTc                                                   <  2:1571724/100‑1 (MQ=255)
                                             gcgcCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCt                                                  <  1:1595545/100‑1 (MQ=255)
                                              cgcCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTg                                                 <  1:223232/100‑1 (MQ=255)
                                                ccTGGGTTGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCAACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAg                                               <  1:1561586/100‑1 (MQ=255)
                                                ccTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAg                                               >  1:1845455/1‑100 (MQ=255)
                                                ccTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAg                                               <  1:2858394/100‑1 (MQ=255)
                                                ccTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAg                                               <  2:1546554/100‑1 (MQ=255)
                                                 cTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAgc                                              <  2:939080/100‑1 (MQ=255)
                                                 cTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAgc                                              <  2:896974/100‑1 (MQ=255)
                                                  tGGGTAGACATCTAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAgcg                                             <  2:255719/100‑1 (MQ=255)
                                                  tGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAgcg                                             >  1:2581541/1‑100 (MQ=255)
                                                   gggTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAgcgc                                            <  1:197860/100‑1 (MQ=255)
                                                    ggTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCt                                           >  2:854129/1‑100 (MQ=255)
                                                    ggTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCt                                           >  1:127481/1‑100 (MQ=255)
                                                     gTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTa                                          <  2:2727072/100‑1 (MQ=255)
                                                      tAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTAt                                         >  2:1020051/1‑100 (MQ=255)
                                                          cATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGc                                     <  1:1144349/100‑1 (MQ=255)
                                                          cATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGc                                     >  2:423576/1‑100 (MQ=255)
                                                          cATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGc                                     >  1:2424645/1‑100 (MQ=255)
                                                          cATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGc                                     >  1:779828/1‑100 (MQ=255)
                                                           aTCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCa                                    <  1:1187794/100‑1 (MQ=255)
                                                            tCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCaa                                   <  2:2691100/100‑1 (MQ=255)
                                                            tCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCaa                                   >  1:2567436/1‑100 (MQ=255)
                                                             cGAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCaaa                                  >  2:1815621/1‑100 (MQ=255)
                                                              gAAGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAAc                                 <  2:2703023/100‑1 (MQ=255)
                                                              gAAGAGATCACCAGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAAc                                 >  2:1912643/1‑100 (MQ=255)
                                                               aaGAGATCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACa                                <  2:1549255/100‑1 (MQ=255)
                                                                  agaTCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGtc                             <  1:1336763/100‑1 (MQ=255)
                                                                   gaTCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGtct                            <  2:1645192/100‑1 (MQ=255)
                                                                   gaTCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGtct                            <  1:205130/100‑1 (MQ=255)
                                                                   gaTCACCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGtct                            >  1:2390826/1‑100 (MQ=255)
                                                                   gaTCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGtct                            >  2:2743674/1‑100 (MQ=255)
                                                                       aCCCGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTg                        <  2:2265144/100‑1 (MQ=255)
                                                                        cccGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGa                       <  1:2784287/100‑1 (MQ=255)
                                                                         ccGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGAc                      <  2:2031552/100‑1 (MQ=255)
                                                                         ccGTAACACCGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGAc                      <  2:2567469/100‑1 (MQ=255)
                                                                                 ccGTTCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTGTCCTGACATCAACCa              <  2:1069726/100‑1 (MQ=255)
                                                                                    ttCGCGAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAggg           <  1:1745900/100‑1 (MQ=255)
                                                                                      cgcgAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAGGGCg         >  2:2171884/1‑100 (MQ=255)
                                                                                       gcgAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAGGGCGt        <  1:1815595/100‑1 (MQ=255)
                                                                                        cgAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAGGGCGtt       <  1:2315834/100‑1 (MQ=255)
                                                                                        cgAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAGGGCGtt       >  1:2214061/1‑100 (MQ=255)
                                                                                         gAAATTGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAGGGCGTTg      <  2:1447210/100‑1 (MQ=255)
                                                                                             ttGTCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAGGGCGTTGACCg  <  1:2541934/100‑1 (MQ=255)
                                                                                                |                                                                                                
ACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTTCGCGAAATTGGCTATGTGCATTCCGACATGGGCTTTGACGCTAACTCCTGTGCGGTTCTGAGCGCTATCGGCAAACAGTCTCCTGACATCAACCAGGGCGTTGACCG  >  NC_000913/3086839‑3087031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑