Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 281,599 C→T G129S (GGC→AGC)  yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,5990CT99.0% 341.0 / NA 102G129S (GGC→AGC) yagACP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (48/53);  total (49/53)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTT  >  NC_000913/281502‑281695
                                                                                                 |                                                                                                
tGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCg                                                                                                <  1:2280515/100‑1 (MQ=255)
 ggCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCgg                                                                                               >  1:2611517/1‑100 (MQ=255)
  gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGt                                                                                              >  2:595073/1‑100 (MQ=255)
   cAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTg                                                                                             <  2:2658212/100‑1 (MQ=255)
      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAg                                                                                          <  1:1308515/100‑1 (MQ=255)
      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAg                                                                                          <  2:2895617/100‑1 (MQ=255)
       gTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAgc                                                                                         <  1:2330390/100‑1 (MQ=255)
        tCCACCGCCAAAAGGAAAGTAGCCCTTAAAATCCATCTGCCAGAGGCAGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAgcg                                                                                        >  1:668456/1‑100 (MQ=255)
         ccACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAgcg                                                                                        <  1:609587/99‑1 (MQ=255)
          cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGcc                                                                                      >  2:588622/1‑100 (MQ=255)
              gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGTCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgc                                                                                  <  2:2753356/100‑1 (MQ=255)
              gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgc                                                                                  >  2:1742757/1‑100 (MQ=255)
              gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgc                                                                                  >  2:4183/1‑100 (MQ=255)
               ccAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgca                                                                                 <  1:1007870/100‑1 (MQ=255)
                 aaaaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgcagc                                                                               >  1:2781876/1‑100 (MQ=255)
                 aaaaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgcagc                                                                               >  2:2582485/1‑100 (MQ=255)
                 aaaaGGAAAGTGGCCCTTAAAATCCAGCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgcagc                                                                               <  1:562659/100‑1 (MQ=255)
                  aaaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgcagca                                                                              >  1:2216140/1‑100 (MQ=255)
                   aaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGcagcag                                                                             >  1:2466666/1‑100 (MQ=255)
                   aaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGcagcag                                                                             >  2:2176091/1‑100 (MQ=255)
                    aGGAAAGTGGCCCTTAAAAACCATCTGCCAGAGGCGGTTCGGAGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAgg                                                                            <  2:1191183/100‑1 (MQ=255)
                     ggAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAgg                                                                            >  1:29686/1‑99 (MQ=255)
                      gAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGcc                                                                          >  1:1429065/1‑100 (MQ=255)
                          gTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTTGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATgg                                                                      >  2:1307177/1‑100 (MQ=255)
                           tGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGc                                                                     <  1:151783/100‑1 (MQ=255)
                            ggCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCg                                                                    >  2:436694/1‑100 (MQ=255)
                              cccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                                                  <  2:220012/100‑1 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 >  1:279751/1‑100 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 >  1:661443/1‑100 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 >  1:555204/1‑100 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 >  1:1324295/1‑100 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 <  2:1010545/100‑1 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 <  1:2830902/100‑1 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 >  1:1542035/1‑100 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 >  1:1097253/1‑100 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGCTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                 <  1:94981/100‑1 (MQ=255)
                                cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                                                >  2:537285/1‑100 (MQ=255)
                                  tAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCAt                                                              <  1:2850628/100‑1 (MQ=255)
                                    aaaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCa                                                            <  1:4183/100‑1 (MQ=255)
                                         cATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTa                                                       >  1:336278/1‑100 (MQ=255)
                                         cATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTa                                                       <  1:1898822/100‑1 (MQ=255)
                                           tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAATCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCTATCGGGTTATg                                                     <  1:1526246/100‑1 (MQ=255)
                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                                    >  2:166665/1‑100 (MQ=255)
                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                                    >  2:649528/1‑100 (MQ=255)
                                             tGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                                    >  2:1999760/1‑99 (MQ=255)
                                             tGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                                    >  1:2375240/1‑99 (MQ=255)
                                              gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                                                  <  2:187997/100‑1 (MQ=255)
                                              gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                                                  >  2:1755848/1‑100 (MQ=255)
                                              gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                                                  >  2:1899341/1‑100 (MQ=255)
                                               ccAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACg                                                 >  1:2410592/1‑100 (MQ=255)
                                                cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACgg                                                <  1:545999/100‑1 (MQ=255)
                                                cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACgg                                                <  2:1082952/100‑1 (MQ=255)
                                                 agagGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGt                                               <  2:2305855/100‑1 (MQ=255)
                                                    ggCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCt                                            >  2:1303033/1‑100 (MQ=255)
                                                     gCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCtn                                           <  1:1275380/100‑2 (MQ=255)
                                                     gCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTg                                           <  1:649522/100‑1 (MQ=255)
                                                         ttCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAgg                                       <  2:534630/100‑1 (MQ=255)
                                                          tCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAgg                                       <  1:1003723/99‑1 (MQ=255)
                                                           cGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAggg                                      >  1:1756236/1‑98 (MQ=255)
                                                           cGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCg                                     >  1:1719701/1‑100 (MQ=255)
                                                            ggCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGCCGGTGCTGAAGGCg                                     >  1:1385275/1‑99 (MQ=255)
                                                            ggCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                                    <  2:1025992/100‑1 (MQ=255)
                                                             gcgcGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggg                                   <  2:1734549/100‑1 (MQ=255)
                                                              cgcgTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGc                                  >  2:427492/1‑100 (MQ=255)
                                                                cgtcgtGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCa                                 <  1:1334702/99‑1 (MQ=255)
                                                                  tcgtGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg                              <  2:1341060/100‑1 (MQ=255)
                                                                   cgtGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgt                             >  2:1832308/1‑100 (MQ=255)
                                                                    gtgaTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg                            <  1:334668/100‑1 (MQ=255)
                                                                       ttCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCCGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGc                         >  2:1827388/1‑100 (MQ=255)
                                                                       ttCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGc                         >  2:1047368/1‑100 (MQ=255)
                                                                       ttCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGc                         <  2:2333095/100‑1 (MQ=255)
                                                                       ttCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGc                         >  2:197007/1‑100 (MQ=255)
                                                                        tCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc                        <  2:1348648/100‑1 (MQ=255)
                                                                        tCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc                        <  1:2156850/100‑1 (MQ=255)
                                                                        tCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGc                         >  1:1393875/1‑99 (MQ=255)
                                                                         cGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc                       <  1:83155/100‑1 (MQ=255)
                                                                         cGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc                       >  1:157310/1‑100 (MQ=255)
                                                                         cGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc                       <  1:512599/100‑1 (MQ=255)
                                                                          gAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc                      <  1:1832282/100‑1 (MQ=255)
                                                                           aaCCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCt                     <  1:1718940/100‑1 (MQ=255)
                                                                            aCCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTg                    <  2:711741/100‑1 (MQ=255)
                                                                             ccGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTgg                   <  2:279755/100‑1 (MQ=255)
                                                                             ccGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTgg                   <  2:136106/100‑1 (MQ=255)
                                                                              cGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGt                  <  1:1307159/100‑1 (MQ=255)
                                                                              cGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGt                  <  2:1324313/100‑1 (MQ=255)
                                                                                gCCCGTGGCGGGAATGCTCGGTGAAGCGCCGGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTcc                <  1:2582452/100‑1 (MQ=255)
                                                                                gCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTcc                >  2:1036418/1‑100 (MQ=255)
                                                                                gCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTcc                <  2:2659692/100‑1 (MQ=255)
                                                                                 cccGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCt               >  1:1756632/1‑100 (MQ=255)
                                                                                 cccGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCt               >  1:2308459/1‑100 (MQ=255)
                                                                                   cGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCg             <  1:2813106/100‑1 (MQ=255)
                                                                                    gTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGa            >  1:918481/1‑100 (MQ=255)
                                                                                     tGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAg           <  1:2178406/100‑1 (MQ=255)
                                                                                       gCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCCATGGTGTGCCCCTGGTCCTCGAGcc         <  2:1765497/100‑1 (MQ=255)
                                                                                        cGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCa        >  2:1489189/1‑100 (MQ=255)
                                                                                         gggAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAg       <  2:29687/100‑1 (MQ=255)
                                                                                          ggAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAgc      <  2:2781912/100‑1 (MQ=255)
                                                                                           gAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAgcg     >  1:1031768/1‑100 (MQ=255)
                                                                                            aaTGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAgcgc    <  2:1871300/100‑1 (MQ=255)
                                                                                              tGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCtt  >  2:1231583/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTT  >  NC_000913/281502‑281695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑