Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 220,849 (T)5→4 100% coding (80/816 nt) metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913220,8450T.88.2% 292.0 / 39.0 85coding (84/816 nt)metQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (6/4);  new base . (35/40);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

ACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCCTTC  >  NC_000913/220747‑220941
                                                                                                  |                                                                                                
aCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtt                                                                                                 >  1:2056363/1‑100 (MQ=255)
 cGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt                                                                                                <  2:2042036/100‑1 (MQ=255)
  gTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt                                                                                               <  1:786468/100‑1 (MQ=255)
   tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttt                                                                                              >  1:850343/1‑100 (MQ=255)
    ccAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttca                                                                                             <  1:484990/100‑3 (MQ=255)
    ccAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttca                                                                                             <  2:137374/100‑3 (MQ=255)
         ccATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTg                                                                                       >  1:1236638/1‑100 (MQ=255)
            tatTTGTCTTTCGCACCTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGAc                                                                                     <  1:2563388/100‑1 (MQ=255)
            tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGAcc                                                                                    <  1:977554/100‑1 (MQ=255)
            tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGAcc                                                                                    >  1:1805070/1‑100 (MQ=255)
             atTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCg                                                                                   <  1:587722/100‑1 (MQ=255)
                 gTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGc                                                                                  >  2:2173867/1‑98 (MQ=255)
                  tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGcc                                                                              <  1:1965830/100‑1 (MQ=255)
                  tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGcc                                                                              <  1:110963/100‑1 (MQ=255)
                  tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGcc                                                                              <  2:2146864/100‑1 (MQ=255)
                   cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGcc                                                                              >  1:553260/1‑100 (MQ=255)
                   cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCt                                                                             >  1:2023385/1‑100 (MQ=255)
                   cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCt                                                                             >  2:171790/1‑100 (MQ=255)
                   cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCt                                                                             >  1:1624127/1‑100 (MQ=255)
                   cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCt                                                                             >  1:2156714/1‑100 (MQ=255)
                     ttCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAc                                                                           <  1:832108/100‑1 (MQ=255)
                      tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                          >  2:23870/1‑100 (MQ=255)
                        gCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTAcca                                                                         >  2:2324961/1‑100 (MQ=255)
                         cAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagt                                                                       >  2:591894/1‑100 (MQ=255)
                         cAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagt                                                                       >  2:1075937/1‑100 (MQ=255)
                          aaCTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtg                                                                      <  1:2094179/100‑1 (MQ=255)
                           aCTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgc                                                                     >  2:2026713/1‑100 (MQ=255)
                            cTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTAccagtgc                                                                     >  2:1118103/1‑100 (MQ=255)
                            cTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgcc                                                                    <  1:860435/100‑1 (MQ=255)
                            cTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgcc                                                                    <  1:2131673/100‑1 (MQ=255)
                            cTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgcc                                                                    <  1:1742425/100‑1 (MQ=255)
                                cTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgccagtg                                                                >  1:1469830/1‑100 (MQ=255)
                                  gcgcGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGAt                                                              >  2:1140610/1‑100 (MQ=255)
                                   cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATc                                                             <  2:2383900/100‑1 (MQ=255)
                                    gcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATc                                                             <  1:1946075/100‑1 (MQ=255)
                                    gcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATcn                                                            <  2:2314566/100‑2 (MQ=255)
                                        cTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATc                                                        <  2:1357361/100‑1 (MQ=255)
                                         ttCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCa                                                       <  2:2232715/100‑1 (MQ=255)
                                          tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAg                                                      >  2:1351287/1‑100 (MQ=255)
                                           cTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAgg                                                     <  2:1947929/100‑1 (MQ=255)
                                           cTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAgg                                                     >  1:2277553/1‑100 (MQ=255)
                                            tGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGAGCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAggg                                                    <  2:2250257/100‑1 (MQ=255)
                                             gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGc                                                   <  2:2482302/100‑1 (MQ=255)
                                             gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGc                                                   >  2:485926/1‑100 (MQ=255)
                                                aCCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTcc                                                <  1:2288587/100‑1 (MQ=255)
                                                 ccTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTccc                                               >  1:87689/1‑100 (MQ=255)
                                                 ccTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTccc                                               >  1:2262343/1‑100 (MQ=255)
                                                     ctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTg                                           <  2:1948156/100‑1 (MQ=255)
                                                      tgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGc                                          <  1:57080/100‑1 (MQ=255)
                                                      tgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGc                                          >  2:1927612/1‑100 (MQ=255)
                                                      tgTTCGGAACAAACAATACCGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCCGTGCCAGTGATCCAATCAGGGATACCACTGc                                          <  2:1687177/100‑1 (MQ=255)
                                                       gTTCGGCACCAACAATCACGCCGACGTTAATGTGGTTTGAATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCAc                                             >  2:1698595/1‑96 (MQ=255)
                                                        ttCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACTTTTGTTAGTGATCCGATCAGGGCTCCCACTGCCg                                        <  2:626529/100‑1 (MQ=255)
                                                          cGGCACCAACAATTTCGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGAGCAGTGCTCCCACTGc                                          >  2:478628/1‑96 (MQ=255)
                                                          cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTGCCACTGCCGCa                                      >  2:2208017/1‑100 (MQ=255)
                                                          cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCa                                      >  1:2026787/1‑100 (MQ=255)
                                                           ggCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCaa                                     <  1:13013/100‑1 (MQ=255)
                                                            gCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCaaa                                    >  1:2531407/1‑100 (MQ=255)
                                                            gCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCaaa                                    >  1:855214/1‑100 (MQ=255)
                                                               ccAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGt                                 >  1:1323958/1‑100 (MQ=255)
                                                                caacaaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAgg                                  >  2:1491566/1‑98 (MQ=255)
                                                                  acaaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGtttt                              <  1:445505/100‑1 (MQ=255)
                                                                   caaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttg                             <  2:788492/100‑1 (MQ=255)
                                                                   caaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttg                             <  1:809243/100‑1 (MQ=255)
                                                                   caaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttg                             <  1:1953648/100‑1 (MQ=255)
                                                                    aaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttga                            <  2:1361082/100‑1 (MQ=255)
                                                                    aaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttga                            <  1:2430057/100‑1 (MQ=255)
                                                                     aTCACGCCGACTTTAATGTGTTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaa                           >  2:1589934/1‑100 (MQ=255)
                                                                     aTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaa                           >  1:188743/1‑100 (MQ=255)
                                                                         cGCCGGCTTTAATGTGGTTTGTATCTTTTCATCCTGACCGCAGCCTTCCTGTGCCAGTGCTCCGATCAGGTCTCCCACTCCCGCAAAGGTtttgaatttg                       <  2:56239/100‑1 (MQ=255)
                                                                             gACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACg                   <  1:591897/100‑1 (MQ=255)
                                                                             gACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACg                   <  1:675335/100‑1 (MQ=255)
                                                                             gACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACg                   <  1:594501/100‑1 (MQ=255)
                                                                               cTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGcc                 >  2:920200/1‑100 (MQ=255)
                                                                               cTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGcc                 >  1:674184/1‑100 (MQ=255)
                                                                                 ttAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCAt               >  2:554509/1‑100 (MQ=255)
                                                                                  tAATGTGGTTTGGCTCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCAt               <  2:2341364/100‑1 (MQ=255)
                                                                                  tAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCACACTGCCGCAAAGGTTTTGACTTTGAACGCCATa              >  1:2171246/1‑100 (MQ=255)
                                                                                    aTGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTTCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATAc             >  1:1208960/1‑100 (MQ=255)
                                                                                    aTGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATAc             <  1:2041816/100‑1 (MQ=255)
                                                                                    aTGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATAcc            <  1:2411069/100‑1 (MQ=255)
                                                                                    aTGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATAcc            <  1:1382236/100‑1 (MQ=255)
                                                                                      gtgGTTTGGATCTTTTAATCCAGACCGCTGCGGACCAGTACCAGTAATAAAAACAGGACAAAACCCGCCGCAAACGATTTGAATTTCAAAGCCATActat          >  2:1655693/1‑97 (MQ=255)
                                                                                        ggTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTAt        <  1:2088467/100‑1 (MQ=255)
                                                                                         gTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTAtt       <  2:1198869/100‑1 (MQ=255)
                                                                                          tttGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTc      >  1:780899/1‑100 (MQ=255)
                                                                                            tGGATCTTTTCATCCTGGCCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCct    <  2:934164/100‑1 (MQ=255)
                                                                                            tGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCct    <  2:2244474/100‑1 (MQ=255)
                                                                                            tGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCct    <  2:1236633/100‑1 (MQ=255)
                                                                                             ggATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCctt   >  2:594317/1‑100 (MQ=255)
                                                                                               aTCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCcttc  >  2:2153882/1‑100 (MQ=255)
                                                                                                  |                                                                                                
ACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCCTTC  >  NC_000913/220747‑220941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: