Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,410,053 (T)9→8 100% intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0450T.89.0% 346.4 / 40.6 100intergenic (+39/‑88)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base T (6/5);  new base . (43/46);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAC  >  NC_000913/4409946‑4410137
                                                                                                   |                                                                                            
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGt                                                                                              <  2:1417556/100‑1 (MQ=255)
   gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                           >  1:1143444/1‑100 (MQ=255)
   gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                           >  1:621563/1‑100 (MQ=255)
   gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                           >  1:1321151/1‑100 (MQ=255)
   gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttt                                                                                           <  1:1680054/100‑1 (MQ=38)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                        >  2:888963/1‑100 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                        <  2:191112/100‑1 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGTATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                       <  2:1389056/100‑4 (MQ=37)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                       >  2:2042587/1‑100 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                       >  1:966239/1‑100 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                       >  1:966224/1‑100 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                       >  1:20446/1‑100 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                       <  2:2503004/100‑4 (MQ=37)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                       <  1:1116961/100‑4 (MQ=37)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttg                                                                                      <  1:1975721/100‑2 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                    <  1:2490720/100‑4 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                    <  1:2042598/100‑4 (MQ=255)
           cGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                    <  2:906286/99‑4 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTAGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                    <  2:1229491/98‑4 (MQ=255)
               tGCTACCGCAATTTGCTTATTTGAAGGTGTGCGCCATCGTTGCTAATTTCTCAGAATTATGAAACGACATCCATATTTGGATGGTTTTTTTTGTCtata                                                                               <  2:2016724/99‑1 (MQ=255)
                gCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGTTTTATGACAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGc                                                                             <  2:491830/100‑1 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTCTAGc                                                                             >  2:1616175/1‑99 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCt                                                                            >  1:1893564/1‑100 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCt                                                                            >  2:872274/1‑100 (MQ=255)
                   aCCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTgg                                                                          >  1:739012/1‑100 (MQ=255)
                    ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGt                                                                         >  1:823587/1‑100 (MQ=255)
                      ggAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTaa                                                                       <  1:1337224/100‑1 (MQ=255)
                       gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAg                                                                      <  1:1237204/100‑1 (MQ=255)
                       gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAg                                                                      <  1:593624/100‑1 (MQ=255)
                        aaTTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGa                                                                     <  2:287066/100‑1 (MQ=255)
                         aTTTGCTTATTTGAAGCGGTGCTCCAGCGCAGCTAATTTCTCATAATTATGAAAAGCCATCCAGATTTGGATGTTTTTTTTTGTCTATAGCTGGTAAGa                                                                     <  2:2232271/99‑1 (MQ=255)
                         aTTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGAt                                                                    >  1:1392655/1‑100 (MQ=255)
                             gCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                >  2:1150877/1‑100 (MQ=255)
                             gCTTATTTGAAGCGGTGCGCCAGCGCAGATAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAt                                                                 >  2:368208/1‑99 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATATCTGGTAAGATAATTa                                                               >  2:316448/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                               >  1:1491668/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATACAGATTTGGATGGTATTTTTTGTCTATAGCTGGTAAAATAGTTa                                                               >  2:2497844/1‑100 (MQ=255)
                                 aTTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                            <  1:1245261/100‑1 (MQ=255)
                                 aTTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                            >  2:1036305/1‑100 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTTTTTTATCTGGTAAGATAATTACGt                                                            >  2:468958/1‑99 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                           >  2:838484/1‑100 (MQ=255)
                                          ggtgatgcAGCGCTGTTAATTTCTCAGTATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaaa                                                    <  1:1513471/93‑1 (MQ=255)
                                          ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                   >  1:2544143/1‑100 (MQ=255)
                                          ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                   <  1:1421828/100‑1 (MQ=255)
                                           gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAata                                                  <  2:637016/100‑1 (MQ=255)
                                             gcgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATAtt                                                <  1:1131257/100‑1 (MQ=255)
                                              cgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTc                                               >  1:1798115/1‑100 (MQ=255)
                                                  aGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                           <  2:131542/100‑1 (MQ=255)
                                                  aGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                           >  2:2175485/1‑100 (MQ=255)
                                                   gcgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGTTGGTAAGATAATTACGTATTGCAAATATTCAtttt                                          <  2:459402/100‑1 (MQ=255)
                                                    cgtaGCTAATTTCTCAGAATTATGAAAAGTCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                             <  2:1281666/93‑1 (MQ=255)
                                                    cgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTtta                                         >  1:1404845/1‑100 (MQ=255)
                                                    cgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTtta                                         <  1:1686766/100‑1 (MQ=255)
                                                     gcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                        <  1:730338/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                       <  2:584419/100‑1 (MQ=255)
                                                       aGCTTATGTCTGTGAATTAAGAAAAGCCATACATATTTTTCTGGTTTTTGCTGTCTATAGCTGGCAAGATAATTACGTATTGCAAATATTCATTttat                                        <  2:1820598/98‑1 (MQ=255)
                                                       aGCTAATTTCTCAGATTTATGAAAAGCCATCCAGATTTTGATGGTTTTTTTTGTCTATATCTGGTAAGATAATTACGTATTGCAAATATTCATTttatta                                      <  2:1644229/100‑1 (MQ=255)
                                                        gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                     >  1:132346/1‑100 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                   <  1:2662/100‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                   <  2:170742/100‑1 (MQ=255)
                                                           aaTTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAatat                                  <  1:257130/100‑1 (MQ=255)
                                                             tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                >  2:793975/1‑100 (MQ=255)
                                                               tctcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaa                              >  1:225742/1‑100 (MQ=255)
                                                               tctcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaa                              <  1:2240161/100‑1 (MQ=255)
                                                                 tcattaTTATGAAAATCCATCCAGATTTGTATGGTTTTTTTTATCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                             <  2:1776791/94‑1 (MQ=255)
                                                                 tcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAc                            >  1:1921112/1‑100 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                           <  1:841201/100‑1 (MQ=255)
                                                                   aGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                          >  1:8888/1‑100 (MQ=255)
                                                                   aGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                          >  2:2508327/1‑100 (MQ=255)
                                                                    gAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTTTTTTTTTTTGTAAGATAATTACATATTGCAAATATTTATTTTATTACTATTTAAAGtt                          >  2:2382559/1‑99 (MQ=255)
                                                                     gacaaaaaaaaACCATCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTaa                        >  1:1308468/8‑100 (MQ=25)
                                                                      gacaaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                       >  1:1769185/7‑100 (MQ=37)
                                                                       acaaaaaaaACCATCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTCATc                      >  1:2134397/6‑100 (MQ=25)
                                                                        tATGTAAATCTATTCAGATTTGGATGTTTTTTTTTTTTTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                     <  2:223689/100‑1 (MQ=255)
                                                                        tATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                      <  1:495636/99‑1 (MQ=255)
                                                                        caaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                     >  1:921653/5‑100 (MQ=38)
                                                                         aaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCta                    >  1:1114495/4‑100 (MQ=38)
                                                                         aTGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCta                    <  2:1763800/100‑1 (MQ=255)
                                                                         aTGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCta                    >  1:532209/1‑100 (MQ=255)
                                                                         aTGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCta                    <  2:1404838/100‑1 (MQ=255)
                                                                          aaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                   >  1:1360698/3‑100 (MQ=38)
                                                                            aaaaGCCATCCAGATTTGGATGGTTTTTTTTTTTTATATCTTGTAATATAATTAAGTATTTCAAATATTCATTTTTTTAATATTTAAATTTAATCTatat                 >  2:1157877/1‑100 (MQ=255)
                                                                            aaaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                 <  1:1600440/100‑1 (MQ=255)
                                                                            aaaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                 >  1:2101692/1‑100 (MQ=255)
                                                                            aaaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                 <  2:2522068/100‑1 (MQ=255)
                                                                             aaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                <  2:1892961/100‑1 (MQ=255)
                                                                                gCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaa             <  1:502584/100‑1 (MQ=255)
                                                                                accATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaa             >  1:373004/2‑100 (MQ=255)
                                                                                accATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGa              >  2:1819070/2‑99 (MQ=255)
                                                                                 ccATCCAAATTTGGATGGTTTTTTTTTTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaat            <  2:1951324/100‑1 (MQ=255)
                                                                                      cAGATTTGGATGGTTTTTTTTTTTTTTTTTTGGTAAGATAATTACGTATTGCAAATATTTGTTTTATTAATATTTAAAGTTATTCTTTATGGAATAatat       >  2:1412044/1‑100 (MQ=255)
                                                                                       aGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata      <  1:1585686/100‑1 (MQ=255)
                                                                                        gATTTGGATGGTTTTTTTTTTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat     >  2:1322178/1‑100 (MQ=255)
                                                                                        gATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat     >  1:13881/1‑100 (MQ=255)
                                                                                         aTTTGGATTGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTGTATTAATATTTAAACTTAATCTATATGGAATAAGATATg    <  2:1165471/100‑1 (MQ=255)
                                                                                         aTTTGGATGGTTTTTTTTGTCTATAGTTGGTAAGATAATTACATATTGCAAATATTAATTATATTAATATTTAAACTTAATCTATATGGAATAATATATg    <  2:742364/100‑1 (MQ=255)
                                                                                         aTTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg    <  2:8184/100‑1 (MQ=255)
                                                                                         aTTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg    >  1:2437822/1‑100 (MQ=255)
                                                                                          tttGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg    <  1:117101/99‑1 (MQ=255)
                                                                                           ttGGATGGTTTTTTTCGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAc  <  1:2562561/100‑1 (MQ=255)
                                                                                                   |                                                                                            
CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAC  >  NC_000913/4409946‑4410137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: