| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,383,727:1 | (A)5→6 | 100% | coding (235/999 nt) | ycjW ← | LacI family putative transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,383,722 | 1 | . | A | 66.2% | 157.0 / 82.3 | 65 | coding (240/999 nt) | ycjW | LacI family putative transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (12/10); new base A (20/23); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGC > NC_000913/1383623‑1383821 | gCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCagag < 1:1111571/100‑1 (MQ=255)gCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCagag < 2:2321267/100‑1 (MQ=255) cATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTAGCGGTGATGGCATGCAAAACTTCAgag‑a > 2:1046186/1‑100 (MQ=255) ccgatctcAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑aa < 2:1902655/93‑1 (MQ=255) tttttGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑aaa < 2:2413337/100‑1 (MQ=255) tttttGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑aaa > 1:1919427/1‑100 (MQ=255) tttGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑aaaa‑a > 2:220720/1‑100 (MQ=255) tGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAACCTTCAGAG‑AAAA‑ACg > 1:2328534/1‑100 (MQ=255) gCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑aaaa‑aacg < 1:898540/100‑4 (MQ=255) naGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGa > 1:499713/2‑100 (MQ=255) cAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑aaagatcgg > 1:1902664/1‑94 (MQ=255) gATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGAtt > 1:1547305/1‑100 (MQ=255) ttctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTAcc < 1:1615142/100‑1 (MQ=255) tctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACccg < 1:1239594/100‑1 (MQ=255) ctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACccg < 1:1443805/100‑1 (MQ=255) ctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACccg > 1:118290/1‑100 (MQ=255) aTTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGtt < 1:222221/100‑1 (MQ=255) gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGtttt > 2:1760801/1‑100 (MQ=255) gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGtttt > 2:1037783/1‑100 (MQ=255) gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGtttt > 1:1086799/1‑100 (MQ=255) gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTg > 1:2098448/1‑100 (MQ=255) gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTACAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGtttt > 2:1396153/1‑100 (MQ=255) tgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTg < 2:1319930/100‑1 (MQ=255) tgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTg < 1:1021529/100‑1 (MQ=255) gCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTgg < 1:1037788/100‑1 (MQ=255) cGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGt < 1:1621518/100‑1 (MQ=255) cGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTc > 1:1391250/1‑100 (MQ=255) gACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCg < 2:410122/100‑1 (MQ=255) cGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGTAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGa < 1:1043112/100‑1 (MQ=255) gTTTTTAAGATAAAGTCAAAACTTCCTTCTTCAGCTTTGGCGGTTATTGAATACAAAACTTCAAAGAAAAA‑ACGGATTTCCCGCCGTAGTTTTGGTCg < 2:1298450/98‑1 (MQ=255) tttGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACg < 1:1140332/100‑1 (MQ=255) ttGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACg > 1:2015950/1‑100 (MQ=255) tGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACggg > 1:2552526/1‑100 (MQ=255) gTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACggg > 1:159154/1‑100 (MQ=255) tAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACACGCCGTAGTTTTGGTCGAACGGGt > 1:828850/1‑100 (MQ=255) aaTATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTa < 1:1300831/100‑1 (MQ=255) aaTATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGACCGGGTa < 2:521351/99‑1 (MQ=255) tCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAAt > 1:923094/1‑100 (MQ=255) gTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc < 1:616638/100‑1 (MQ=255) gTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc > 1:2307210/1‑100 (MQ=255) gTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc > 1:2130614/1‑100 (MQ=255) tCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc < 2:118289/100‑1 (MQ=255) tCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc < 1:2122829/100‑1 (MQ=255) tCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc > 1:1320276/1‑100 (MQ=255) tCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc < 1:1547496/100‑1 (MQ=255) cGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCa > 1:2107344/1‑100 (MQ=255) gAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCAt < 2:75234/100‑1 (MQ=255) ccTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATgg < 1:468177/100‑1 (MQ=255) cttcttcttCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATgg > 1:1561981/1‑100 (MQ=255) cttcttcttCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGc > 1:1357087/1‑100 (MQ=255) tcttgcacAGCTTTGGCGGTGATGCCATGCACAAATTAAGAG‑AAAATACTGATTCCCCGCCGTAGTTTTGTTCGAACGGGTAGAAATCACCATAATGGCa < 2:899816/93‑1 (MQ=255) cttcctcAGCTGTGCCGGTGATGTCAGGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTGTGGTCGAACGGGTAGAAATCACCATAATGGCAt < 2:1486325/100‑1 (MQ=255) ttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaa > 2:1346894/1‑100 (MQ=255) cAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAc < 2:2500396/100‑1 (MQ=255) aGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAcc < 1:2008185/100‑1 (MQ=255) gCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAccc > 1:990843/1‑100 (MQ=255) gCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAccc < 1:2428676/100‑1 (MQ=255) gCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCg > 1:123084/1‑100 (MQ=255) tttGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGa < 2:82261/100‑1 (MQ=255) tGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAag < 2:2452642/100‑1 (MQ=255) gCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTGGAAATCACCATAATGGCATCAAAACCCGAAGAgg < 2:1498096/100‑1 (MQ=255) gCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAgg < 1:2276671/100‑1 (MQ=255) cGGTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAgg > 2:1331720/1‑100 (MQ=255) ggTGATGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTATTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAgg > 2:1550845/1‑99 (MQ=255) tGGCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAgcg < 2:1769222/100‑1 (MQ=255) gCATGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAgcgcg > 2:1981551/1‑100 (MQ=255) aTGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGcgcgcg > 1:2538215/1‑100 (MQ=255) tGCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCgg > 2:2234910/1‑100 (MQ=255) gCAAAACTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCggg < 1:2294523/100‑1 (MQ=255) gCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGc > 1:1955908/1‑100 (MQ=255) aaaaCTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGcc < 2:1086794/100‑1 (MQ=255) aCTTCAGAGAAAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAAAGAGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCACAGGCAAGc > 2:598876/1‑100 (MQ=255) cAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGtt > 2:945490/1‑100 (MQ=255) cAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGtt < 1:1040615/100‑1 (MQ=255) gag‑aAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTg > 1:264411/1‑100 (MQ=255) g‑aAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGc > 2:1411784/1‑100 (MQ=255) | GCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAG‑AAAA‑ACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGC > NC_000913/1383623‑1383821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |