BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,809,774:1	(T)_7→8	100%	coding (158/738 nt)	ygaZ →	putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,809,767	1	.	T	100.0%	311.5 / ‑6.7	90	V51F (GTT→TTT)	ygaZ	putative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand): ref base . (0/1); major base T (34/54); minor base A (1/0); total (35/55)

Only 100 of 104 total aligned reads displayed.

GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTG  >  NC_000913/2809668‑2809859

ggATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCg                                                                                                 <  2:345364/100‑1 (MQ=255)

 gATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑t                                                                                              <  1:552465/100‑1 (MQ=255)

  aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tt                                                                                             <  2:1059199/100‑1 (MQ=255)

    gCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttt                                                                                           <  2:921961/100‑1 (MQ=255)

    gCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttt                                                                                           <  1:2185800/100‑1 (MQ=255)

     cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttt                                                                                          >  1:1856775/1‑100 (MQ=255)

     cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttt                                                                                          >  2:1284438/1‑100 (MQ=255)

     cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttt                                                                                          >  2:2209873/1‑100 (MQ=255)

      aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttttt                                                                                         <  1:73109/100‑1 (MQ=255)

       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttttt‑t                                                                                       >  1:1574917/1‑100 (MQ=255)

       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTAGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttttt‑t                                                                                       >  1:2592043/1‑100 (MQ=255)

         gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttttt‑ttc                                                                                     <  1:2098933/100‑3 (MQ=255)

          aCAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCTTCCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttttt‑ttct                                                                                    <  2:1178986/100‑4 (MQ=255)

             gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCt                                                                                  <  2:1992261/100‑1 (MQ=255)

              tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTg                                                                                 <  2:78125/100‑1 (MQ=255)

               ttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGc                                                                                <  1:1314692/100‑1 (MQ=255)

                tACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTg                                                                                 >  2:1125922/1‑98 (MQ=255)

                tACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttttt‑ttcttcttca                                                                              >  2:1135461/1‑91 (MQ=255)

                 aCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGcat                                                                              >  1:1128961/1‑100 (MQ=255)

                   cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGcatca                                                                            <  2:2492206/100‑1 (MQ=255)

                     aTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGACTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCAtt                                                                          <  2:1669096/100‑1 (MQ=255)

                      ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCAttt                                                                         <  1:528337/100‑1 (MQ=255)

                       tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTa                                                                        <  1:1508956/100‑1 (MQ=255)

                         ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATg                                                                      >  1:2040319/1‑100 (MQ=255)

                         ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATg                                                                      <  1:1538937/100‑1 (MQ=255)

                         ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATg                                                                      <  1:2234812/100‑1 (MQ=255)

                           attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCa                                                                    >  2:676163/1‑100 (MQ=255)

                             taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAgg                                                                  >  1:558151/1‑100 (MQ=255)

                              aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGgc                                                                 >  1:716510/1‑100 (MQ=255)

                              aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGgc                                                                 >  1:15859/1‑100 (MQ=255)

                              aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGgc                                                                 >  1:1462541/1‑100 (MQ=255)

                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGgcg                                                                >  1:1938855/1‑100 (MQ=255)

                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGgcg                                                                <  2:1128956/100‑1 (MQ=255)

                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGgcg                                                                >  1:1395325/1‑100 (MQ=255)

                                ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGgcgc                                                               <  2:1189998/100‑1 (MQ=255)

                                 tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGcgcg                                                              <  2:806536/100‑1 (MQ=255)

                                 tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGcgcg                                                              >  1:243774/1‑100 (MQ=255)

                                 tataTGCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGgc                                                                 <  2:888859/97‑1 (MQ=255)

                                   tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAg                                                            <  1:1293932/100‑1 (MQ=255)

                                   tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAg                                                            >  1:1192349/1‑100 (MQ=255)

                                   tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAg                                                            <  2:34480/100‑1 (MQ=255)

                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGc                                                           <  2:445814/100‑1 (MQ=255)

                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGcc                                                          <  1:1042786/100‑1 (MQ=255)

                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGcc                                                          <  1:2025881/100‑1 (MQ=255)

                                       ccGGTGTCCTTTGCCTTCGGTCTGAATTTGACCCGTCTGGGATGCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGcc                                                          <  2:2281539/98‑1 (MQ=255)

                                       ccGGGGGCCTGTGTGTGCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCAGCATTTATGCAGGCGCGAg                                                            <  2:12144/96‑1 (MQ=255)

                                        cGGTGGCCTCTGCGTCAGGAAAAAATAAGACCCGTCTGGGATTCCCTCCTCTCGAAAGCGA‑ATTTTT‑TCTTCTGCATCATATATACATgcact                                                              >  2:273370/1‑90 (MQ=255)

                                         ggTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGtt                                                      >  1:829477/1‑100 (MQ=255)

                                          gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                     <  1:1333999/100‑1 (MQ=255)

                                           tGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                    <  2:1090537/100‑1 (MQ=255)

                                           tGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                    <  2:243771/100‑1 (MQ=255)

                                           tGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                    <  2:1090442/100‑1 (MQ=255)

                                            ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGt                                                   <  1:2256617/100‑1 (MQ=255)

                                             gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                  <  1:424869/100‑1 (MQ=255)

                                             gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                  <  2:1343016/100‑1 (MQ=255)

                                                  tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc                                             >  1:800651/1‑100 (MQ=255)

                                                  tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc                                             >  2:1792499/1‑100 (MQ=255)

                                                   gCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAcc                                            >  1:1748885/1‑100 (MQ=255)

                                                    cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                           >  1:380302/1‑100 (MQ=255)

                                                    cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                           >  1:1681270/1‑100 (MQ=255)

                                                    cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                           >  2:659478/1‑100 (MQ=255)

                                                    cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                           <  2:957109/100‑1 (MQ=255)

                                                    cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                           >  1:2419245/1‑100 (MQ=255)

                                                     gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTT‑TTTTTTTTTTCATTTATGCCGGCGCCAGCCATTTTGTCATTACcg                                           >  2:937584/1‑100 (MQ=255)

                                                     gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                          <  1:1593530/100‑1 (MQ=255)

                                                        cGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGAt                                       >  1:1688628/1‑100 (MQ=255)

                                                         ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTTCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGAt                                       >  2:1676279/1‑99 (MQ=255)

                                                         ggTCTGAATGCGACCCGTCTGGGATGCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgcg                                         <  2:1620346/97‑1 (MQ=255)

                                                           tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGACAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                    <  1:1825260/100‑1 (MQ=255)

                                                           tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                    >  1:2417441/1‑100 (MQ=255)

                                                            cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                   >  1:1477717/1‑100 (MQ=255)

                                                             tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                   >  2:1466731/1‑99 (MQ=255)

                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                 <  1:1574325/100‑1 (MQ=255)

                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                 <  1:1907432/100‑1 (MQ=255)

                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                 <  1:414730/100‑1 (MQ=255)

                                                               aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                <  2:1688619/100‑1 (MQ=255)

                                                               aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                <  1:763377/100‑1 (MQ=255)

                                                               aaTGCGACCCGTCCGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                >  1:1750691/1‑100 (MQ=255)

                                                                 tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGc                              >  1:1951361/1‑100 (MQ=255)

                                                                    gACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                           >  1:1006161/1‑100 (MQ=255)

                                                                      cccGTCTGGGATTCTCGCCTCTCGAAAGCGTTTTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                          <  2:569099/100‑1 (MQ=255)

                                                                       ccGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGag                        <  1:2325616/100‑1 (MQ=255)

                                                                       ccGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGa                         >  1:91218/1‑99 (MQ=255)

                                                                       ccGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTGTCGCCTGCATCATTTAGGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGag                        <  1:1488989/100‑1 (MQ=255)

                                                                        cGTCTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                       <  2:1951351/100‑1 (MQ=255)

                                                                           cTGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtagt                    <  2:105246/100‑1 (MQ=255)

                                                                            tGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt                   <  2:2480953/100‑1 (MQ=255)

                                                                            tGGGATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt                   <  2:1799063/100‑1 (MQ=255)

                                                                             gggATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                  <  1:1451368/100‑1 (MQ=255)

                                                                             gggATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                  <  1:2521996/100‑1 (MQ=255)

                                                                              ggATTCTCTCCTCTCGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggagatcggaag                 >  2:1904906/1‑90 (MQ=255)

                                                                                     ctcctctcGAAAGCGT‑TTTTTT‑TTTTCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTGCGTGAAtt           >  2:55622/1‑99 (MQ=255)

                                                                                      tcctctcGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc         <  2:549211/100‑1 (MQ=255)

                                                                                      tcctctcGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc         <  1:1152898/100‑1 (MQ=255)

                                                                                      tcctctcGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc         <  1:2209885/100‑1 (MQ=255)

                                                                                       cctctcGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct        >  2:797332/1‑100 (MQ=255)

                                                                                       cctctcGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc         <  1:2343689/99‑1 (MQ=255)

                                                                                        ctctcGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg       <  1:1949219/100‑1 (MQ=255)

                                                                                         tctcGAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc      <  1:2268138/100‑1 (MQ=255)

                                                                                             gAAAGCGT‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg  >  2:672688/1‑100 (MQ=255)

GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTT‑TCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTG  >  NC_000913/2809668‑2809859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`