Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,866,592:1 +C 100% coding (960/993 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,866,5881.C96.7% 324.7 / 3.8 92coding (964/993 nt)rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (56/33);  total (58/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACA  >  NC_000913/2866491‑2866682
                                                                                                  |                                                                                                
tCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGcc                                                                                               >  2:1791328/1‑100 (MQ=255)
tCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGcc                                                                                               >  1:1847416/1‑100 (MQ=255)
tCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGc                                                                                                 >  2:2593729/1‑99 (MQ=255)
 cGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccc                                                                                             <  1:719033/100‑1 (MQ=255)
     ccTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccccctg                                                                                         <  2:17888/100‑4 (MQ=255)
      cTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccccctgc                                                                                        >  2:1645111/1‑96 (MQ=255)
        tttATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGtt                                                                                      <  1:726627/100‑1 (MQ=255)
            ttGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGc                                                                                   <  1:1078315/100‑1 (MQ=255)
            ttGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGc                                                                                   <  2:1280563/100‑1 (MQ=255)
             tgtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCa                                                                                  <  2:983071/100‑1 (MQ=255)
             tgtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCa                                                                                  >  1:694922/1‑100 (MQ=255)
             tgtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCa                                                                                  <  1:2487211/100‑1 (MQ=255)
             tgtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCa                                                                                  <  1:1648783/100‑1 (MQ=255)
             tgtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCa                                                                                  <  1:1237930/100‑1 (MQ=255)
              gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 <  2:1764984/100‑1 (MQ=255)
              gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 >  1:2147151/1‑100 (MQ=255)
               tgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAgg                                                                                <  2:1046115/100‑1 (MQ=255)
                gCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCACCCTGCGTTTGCAGGa                                                                               >  1:670721/1‑100 (MQ=255)
                 cacaGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAt                                                                              >  2:1358423/1‑100 (MQ=255)
                 cacaGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAt                                                                              <  1:828506/100‑1 (MQ=255)
                  acaGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTCCTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAtt                                                                             >  1:1682501/1‑100 (MQ=255)
                   caGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAttt                                                                            >  1:1386728/1‑100 (MQ=255)
                   caGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAttt                                                                            >  1:1386715/1‑100 (MQ=255)
                   caGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAttt                                                                            >  2:1218230/1‑100 (MQ=255)
                      aaaaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcgc                                                                         <  1:412069/100‑1 (MQ=255)
                      aaaaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcgc                                                                         <  1:1953006/100‑1 (MQ=255)
                       aaaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcgcg                                                                        <  1:134417/100‑1 (MQ=255)
                        aaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCgcgc                                                                       <  2:812348/100‑1 (MQ=255)
                          ggCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCaa                                                                     >  1:2245676/1‑100 (MQ=255)
                          ggCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCaa                                                                     >  1:995770/1‑100 (MQ=255)
                             tccgatctcTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACg                                                                  <  1:675391/92‑1 (MQ=255)
                             cAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACg                                                                  >  1:2453361/1‑100 (MQ=255)
                               gCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGgc                                                                >  2:1653947/1‑100 (MQ=255)
                                 cTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGgcgc                                                              <  2:971109/100‑1 (MQ=255)
                                 cTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGgcgc                                                              >  2:802896/1‑100 (MQ=255)
                                   cGCTTGAGACTGGCCTTTCTGACGGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            >  2:786248/1‑100 (MQ=255)
                                   cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTATTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            >  1:527324/1‑100 (MQ=255)
                                   cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            <  1:2551658/100‑1 (MQ=255)
                                    gCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAgg                                                           >  1:684233/1‑100 (MQ=255)
                                    gCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAgg                                                           >  1:572622/1‑100 (MQ=255)
                                     cTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACgagatcgga                                                          >  2:675387/1‑92 (MQ=255)
                                      ttGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGcc                                                         <  1:802149/100‑1 (MQ=255)
                                       tGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCt                                                        >  1:490208/1‑100 (MQ=255)
                                         agaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTc                                                      <  2:963560/100‑1 (MQ=255)
                                         agaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTc                                                      >  1:228212/1‑100 (MQ=255)
                                          gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     >  1:1278269/1‑100 (MQ=255)
                                          gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     <  1:193427/100‑1 (MQ=255)
                                          gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     <  1:73533/100‑1 (MQ=255)
                                          gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     >  2:919934/1‑100 (MQ=255)
                                           aCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCaa                                                    >  1:2516250/1‑100 (MQ=255)
                                            ntGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     >  2:2294313/2‑98 (MQ=255)
                                            cTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAc                                                   >  2:427572/1‑100 (MQ=255)
                                             tGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc                                                  >  2:228184/1‑100 (MQ=255)
                                             tGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc                                                  <  1:1248784/100‑1 (MQ=255)
                                               gCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTg                                                >  1:264582/1‑100 (MQ=255)
                                                ccTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGa                                               >  2:2517896/1‑100 (MQ=255)
                                                 cTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGaa                                              >  1:488197/1‑100 (MQ=255)
                                                     cTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCACCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTg                                          >  1:555132/1‑100 (MQ=255)
                                                       gACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        >  1:2532422/1‑100 (MQ=255)
                                                       gACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        <  1:2253459/100‑1 (MQ=255)
                                                         cAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGa                                      >  1:245112/1‑100 (MQ=255)
                                                           gATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac                                    >  1:832620/1‑100 (MQ=255)
                                                           gATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac                                    <  2:394081/100‑1 (MQ=255)
                                                             tGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGcctcacc                                  >  2:2410490/1‑94 (MQ=255)
                                                               cttacttaCTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGt                                >  1:15773/1‑100 (MQ=255)
                                                                  acttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTc                              >  1:553995/1‑99 (MQ=255)
                                                                   cttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc                            >  1:2323835/1‑100 (MQ=255)
                                                                    ttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACg                           <  1:238720/100‑1 (MQ=255)
                                                                      actCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         <  1:1284996/100‑1 (MQ=255)
                                                                       ctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGt                        >  2:1506308/1‑100 (MQ=255)
                                                                        tCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTg                       <  2:53020/100‑1 (MQ=255)
                                                                        tCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTg                       <  2:1228330/100‑1 (MQ=255)
                                                                         cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  2:1487927/1‑100 (MQ=255)
                                                                         cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  1:2583066/1‑100 (MQ=255)
                                                                         cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGACGAACACGTTCACGGGTGa                      >  1:1480184/1‑100 (MQ=255)
                                                                          gcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTggg                     >  1:2145973/1‑98 (MQ=255)
                                                                            ggAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGc                   <  2:363302/100‑1 (MQ=255)
                                                                               aCAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCaa                >  1:2324797/1‑100 (MQ=255)
                                                                                cAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGACTTCGCGCAAACGGCGCAGGCCTTCAACCTGAACCTGGCGAACACGTTCACGCGTGCGGCCAAt               >  2:264032/1‑100 (MQ=255)
                                                                                 ngcgcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAtt              >  1:894656/2‑100 (MQ=255)
                                                                                 aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAtt              >  2:523565/1‑100 (MQ=255)
                                                                                 aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAt               >  2:1478154/1‑99 (MQ=255)
                                                                                 aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAACTGAATCTGGAGAATACGTTCACGGGTGAGGCCAttt              >  2:1975419/1‑100 (MQ=255)
                                                                                 aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCACAAACGGCGCAGGCCTTCACCCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAtt              >  2:1422974/1‑100 (MQ=255)
                                                                                  gcgcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAttt             >  1:2420792/1‑100 (MQ=255)
                                                                                    gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           <  1:1764162/100‑1 (MQ=255)
                                                                                     cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          <  2:2245663/100‑1 (MQ=255)
                                                                                     cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          >  1:1547518/1‑100 (MQ=255)
                                                                                     cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          <  1:523569/100‑1 (MQ=255)
                                                                                     cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          >  1:695608/1‑100 (MQ=255)
                                                                                       tCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGa        >  2:1228633/1‑100 (MQ=255)
                                                                                       tCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGa        >  1:890538/1‑100 (MQ=255)
                                                                                         gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      >  2:1426987/1‑100 (MQ=255)
                                                                                         gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      >  2:137299/1‑100 (MQ=255)
                                                                                         gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      >  1:1389848/1‑100 (MQ=255)
                                                                                          atatTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCt     <  1:2391333/100‑1 (MQ=255)
                                                                                           tatTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTa    >  2:1639156/1‑100 (MQ=255)
                                                                                             ttCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACa  <  2:1480176/100‑1 (MQ=255)
                                                                                                  |                                                                                                
TCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACA  >  NC_000913/2866491‑2866682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: