BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,379,266:1	(C)_7→8	100%	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	92.8%	322.6 / 19.5	97	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (6/1); new base C (44/46); total (50/47)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

cGCACAGCGTAGTCAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGtt‑‑t                                                                                                       >  2:1614570/1‑100 (MQ=255)

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGtt‑‑t                                                                                                       <  1:2423366/100‑1 (MQ=255)

 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c                                                                                                   <  1:37225/100‑1 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c‑c                                                                                                 <  2:1453652/100‑1 (MQ=255)

   acaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c‑cc                                                                                                >  1:205511/1‑100 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c‑cc‑c                                                                                              >  2:317233/1‑100 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c‑cc‑c                                                                                              >  1:1932518/1‑100 (MQ=255)

     aGCGTAGTAAAAGAGACCGCCAGCTTCTTGTTCTCGACTTCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c‑cc‑c‑c                                                                                            <  1:1517765/100‑1 (MQ=255)

      gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c‑cc‑c‑cc                                                                                           >  1:2334714/1‑100 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c‑cc‑c‑cc‑ccgcc                                                                                     <  1:675782/100‑5 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑c‑cc‑c‑cc‑ccgcc                                                                                     <  1:1688696/100‑5 (MQ=255)

            taAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑C‑CC‑CACC‑CGCCa                                                                                     >  1:890282/1‑100 (MQ=255)

               tccgatctgCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAgcg                                                                                  <  2:1814796/92‑1 (MQ=255)

               aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAgcg                                                                                  <  1:1128769/100‑1 (MQ=255)

               aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAgcg                                                                                  <  2:729308/100‑1 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAgcgc                                                                                 <  1:348406/100‑1 (MQ=255)

                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGcc                                                                                >  2:665319/1‑100 (MQ=255)

                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGcc                                                                                >  2:657109/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCCGCGCCa                                                                               >  2:2562652/1‑99 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCaa                                                                              >  2:1886201/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAAcc                                                                            <  1:1306962/100‑1 (MQ=255)

                      cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCa                                                                           <  1:1358294/100‑1 (MQ=255)

                      cGCCAGCGTCTGGTTCTCGACCCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGGGCCAACCa                                                                           >  2:359030/1‑100 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑C‑CC‑C‑CC‑CGCCAGCGCCAAcc                                                                            >  2:2410073/1‑99 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAgcgagatcgga                                                                          >  1:1814805/1‑92 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCAt                                                                          >  2:52046/1‑100 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑C‑CC‑CACCGCGCCAGCGCCAACCa                                                                           >  2:415582/1‑100 (MQ=255)

                         taGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAg                                                                        <  1:1898022/99‑1 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAga                                                                       >  2:1898013/1‑99 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGtt                                                                      <  2:888949/100‑1 (MQ=255)

                            cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGttt                                                                     >  2:2304867/1‑100 (MQ=255)

                            cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGttt                                                                     >  2:2485562/1‑100 (MQ=255)

                             gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTa                                                                    <  1:2393495/100‑1 (MQ=255)

                             gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑C‑CCAC‑CC‑CGCCAGCGCCAACCATAGTTTa                                                                    >  1:875614/1‑100 (MQ=255)

                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTAt                                                                   <  2:293471/100‑1 (MQ=255)

                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATCTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTa                                                                    >  2:202505/1‑99 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATcc                                                                 >  1:2592057/1‑100 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGCTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATcc                                                                 <  2:1214669/100‑1 (MQ=255)

                                   ttCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCAtt                                                              >  1:918744/1‑100 (MQ=255)

                                   ttCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCAtt                                                              <  1:2479849/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:454509/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  1:1942185/100‑1 (MQ=255)

                                       cGACGCCGATTGTTGCTAATGGCCGATGGTTGATTTCTCACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑CGCT‑C‑CA‑CGCCAGCGCGAACCATATTTTATCCATTTg                                                            >  2:2519812/1‑98 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  1:1208276/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  1:255754/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACAATAGTTTATCCATTTgcg                                                          >  2:1726797/1‑100 (MQ=255)

                                          cGCCCATTGTTGCTAATGGCCGATGGTTCAGTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACACTAGCTCATCCCTTTGcccc                                                        >  2:353333/1‑99 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCg                                                      >  1:1535622/1‑100 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                     <  1:1629583/100‑1 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                   <  1:292564/100‑1 (MQ=255)

                                                    tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  1:176417/1‑100 (MQ=255)

                                                      cTAATTGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCgg                                           <  2:1161199/100‑2 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                           <  1:1846653/100‑1 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                       <  2:1882986/100‑1 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                       >  2:1734504/1‑100 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAgn                                      <  1:283876/100‑2 (MQ=255)

                                                            gCCAATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:593934/100‑1 (MQ=255)

                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                    >  2:2556987/1‑100 (MQ=255)

                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                    >  1:2428043/1‑100 (MQ=255)

                                                              cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   <  1:1060578/100‑1 (MQ=255)

                                                               gATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCGTAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                  <  1:733383/100‑1 (MQ=255)

                                                               gATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                  <  1:1208576/100‑1 (MQ=255)

                                                               gATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                  <  1:1719179/100‑1 (MQ=255)

                                                                  ggTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                               >  1:2558497/1‑100 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             >  2:822838/1‑100 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                            <  2:31790/100‑1 (MQ=255)

                                                                     tGATTTCTAACGTCGGGGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCAGGGTTTCATCGTGAAGTGGCGAAAGcc                            <  1:1546615/100‑1 (MQ=255)

                                                                      gATTTCTAACGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCca                           >  2:464994/1‑100 (MQ=255)

                                                                      gATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑C‑CCTC‑CA‑TTCCAGCGCTAAACATAGTTTATTCATTTGCGCCCAGGTTTCATCGTGAAGTGGCGAAAGCca                           >  2:608983/1‑100 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCGTT‑‑TCC‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          <  1:2410086/100‑1 (MQ=255)

                                                                             aaCGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                    >  2:2327892/1‑100 (MQ=255)

                                                                              aCGTCGGTGAATTTTCTCGTT‑‑TCCCC‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                     >  2:488444/1‑100 (MQ=255)

                                                                               cGTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                  >  1:2294644/1‑100 (MQ=255)

                                                                                gTCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                 >  2:23077/1‑100 (MQ=255)

                                                                                 tCGGTGAATTTTCTCGTTCCC‑‑‑C‑CC‑C‑CC‑CGCCCGCCCCCACCATTGTTTTTCCCTTTTCGCCCCGGCCTCCTCCCTCAGTGGCGaaaac                             >  2:1057847/1‑86 (MQ=255)

                                                                                 tCGGTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCt                <  2:1493587/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga              <  1:23077/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               >  2:53059/1‑99 (MQ=255)

                                                                                   ggTGAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCATGAAGTGGCGAAAGCCACAGATGCGCCtga              <  1:2588276/100‑1 (MQ=255)

                                                                                     tgtctttcCTCGTT‑‑CC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt            <  1:125682/96‑1 (MQ=255)

                                                                                      gAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           <  1:188666/100‑1 (MQ=255)

                                                                                      gAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           <  1:88321/100‑1 (MQ=255)

                                                                                      gAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           <  2:2002570/100‑1 (MQ=255)

                                                                                      gAATTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           <  1:692866/100‑1 (MQ=255)

                                                                                       aaTTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtga          >  2:592321/1‑100 (MQ=255)

                                                                                        aTTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:2437351/100‑1 (MQ=255)

                                                                                        aTTTTCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  2:820100/1‑100 (MQ=255)

                                                                                         ttttCTCGTT‑‑TCC‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  2:1535613/100‑1 (MQ=255)

                                                                                         ttttCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGCTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  2:2115787/1‑99 (MQ=255)

                                                                                          tttCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg       <  1:982109/100‑1 (MQ=255)

                                                                                          tttCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATACATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg       >  1:247866/1‑100 (MQ=255)

                                                                                           ttCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt      >  1:797081/1‑100 (MQ=255)

                                                                                           ttCTCGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt      >  2:963835/1‑100 (MQ=255)

                                                                                            tctcGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa     <  1:2415128/100‑1 (MQ=255)

                                                                                             ctcGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  1:293723/1‑100 (MQ=255)

                                                                                             ctcGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  2:1871254/1‑100 (MQ=255)

                                                                                             ctcGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  1:961753/1‑100 (MQ=255)

                                                                                              tcGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATACTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg   <  1:908441/100‑1 (MQ=255)

                                                                                               cGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  >  1:384012/1‑100 (MQ=255)

                                                                                               cGTT‑‑TC‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATGTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  <  2:535449/100‑1 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑‑T‑‑‑C‑CC‑C‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`