Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,143,641 G→A 100% H243Y (CAT→TAT)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,6410GA98.5% 290.4 / ‑4.4 91H243Y (CAT→TAT) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (33/55);  total (35/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143544‑1143736
                                                                                                 |                                                                                               
aTCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACt                                                                                               <  2:1855372/100‑1 (MQ=255)
 tCTGTGACTCGATCAGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCCGCTGAAATCCGGGCGACCTAATGCAGCGATATACTg                                                                                              >  2:29691/1‑100 (MQ=255)
   tgtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGAc                                                                                            >  1:1584824/1‑100 (MQ=255)
    gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg                                                                                           >  1:1074457/1‑100 (MQ=255)
    gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg                                                                                           <  1:935120/100‑1 (MQ=255)
    gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg                                                                                           >  1:570615/1‑100 (MQ=255)
      gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         >  1:173539/1‑100 (MQ=255)
      gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         <  1:2242819/100‑1 (MQ=255)
       aCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGc                                                                                        >  1:2577649/1‑100 (MQ=255)
        cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  2:683492/100‑1 (MQ=255)
        cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  1:465057/100‑1 (MQ=255)
             tCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTc                                                                                  <  1:1420732/100‑1 (MQ=255)
               tGGTAGTGGTTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTTCTGCTGAAATCCGGTCGACCTAATGCAGCGATATACTGACGTGCCAg                                                                                     <  2:2158019/95‑1 (MQ=255)
                gttAGTGGCTGAACAGCGTGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTTACGTGCCAGTTCg                                                                                 <  2:1294557/96‑1 (MQ=255)
                ggTATTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  1:1942044/100‑1 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 >  2:1312827/1‑98 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  1:1279688/100‑1 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               >  1:707057/1‑100 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  2:476249/100‑1 (MQ=255)
                 gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc                                                                              <  1:513035/100‑1 (MQ=255)
                 gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc                                                                              <  1:2221731/100‑1 (MQ=255)
                   aGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCAc                                                                            <  1:1830410/100‑1 (MQ=255)
                      ggCTGAACAGCGGGGTCTCGCCGGTGTACAGTTTGAGGTTTCTTCTGAGATCCTGGCGACCTAGTGCATCGATGTGCTGACGTGCCGGTTCGAGCACttt                                                                         >  2:2171690/1‑100 (MQ=255)
                        cTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCg                                                                       <  2:1338382/100‑1 (MQ=255)
                           aaCGGCGGGATCTCGCGGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCGAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGt                                                                    <  2:524605/100‑1 (MQ=255)
                           aaCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGt                                                                    >  1:1849895/1‑100 (MQ=255)
                            aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGtt                                                                   <  2:2189377/100‑1 (MQ=255)
                            aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGtt                                                                   >  1:1286279/1‑100 (MQ=255)
                             cAGCGGGATCTCGCCGGTGTGGAGGTTGATTTTGCTGGTGAAATCCTTGCGACGTAGTGCAGCTTTATACTGATGTGCCAGGTCTCGCAATTTCgg                                                                      >  2:516351/1‑96 (MQ=255)
                             cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  >  1:1853845/1‑100 (MQ=255)
                             cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  <  1:1377759/100‑1 (MQ=255)
                             cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  <  2:1240799/100‑1 (MQ=255)
                              aGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTAt                                                                 <  2:1892417/100‑1 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGatat                                                                 >  1:899448/1‑99 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTAt                                                                 >  1:2089082/1‑99 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:344750/100‑1 (MQ=255)
                               gCGGGAGCTCGCCGGTGGACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:2122647/100‑1 (MQ=255)
                                cGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:391990/99‑1 (MQ=255)
                                cGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCg                                                               <  2:2420384/100‑1 (MQ=255)
                                  ggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGGTATCCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGAt                                                             >  2:334051/1‑100 (MQ=255)
                                  ggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGAt                                                             <  2:2309023/100‑1 (MQ=255)
                                  ggAGCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGAt                                                             <  2:1385602/100‑1 (MQ=255)
                                   gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa                                                            <  1:1856113/100‑1 (MQ=255)
                                     tctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAg                                                          <  1:2148908/100‑1 (MQ=255)
                                       tcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGa                                                        <  1:1944235/100‑1 (MQ=255)
                                       tcGCCGGTGTACAATTTGATTTTGCTGCTGAAATCCGGCCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGa                                                        >  2:1603919/1‑100 (MQ=255)
                                        cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGa                                                        <  1:981067/99‑1 (MQ=255)
                                        cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAt                                                       <  1:2515797/100‑1 (MQ=255)
                                            ggTGTACAGTTTGATTTTGCTGTTGAAATCCGGGCGACCTAATGCAGCTATATACTGACGTGCTAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCg                                                   <  2:711656/100‑1 (MQ=255)
                                                tACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa                                               <  1:1457525/100‑1 (MQ=255)
                                                  ctgTTTGATTTTGCTGCTGAAATCCGGGCGCCTTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                             <  2:116302/98‑1 (MQ=255)
                                                   aGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATgn                                            <  1:1857991/100‑2 (MQ=255)
                                                   aGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGt                                            <  1:1704998/100‑1 (MQ=255)
                                                   aGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCAGATGt                                            >  2:2072103/1‑100 (MQ=255)
                                                    ggttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGAAATGCCAGTTCGAGCACTTTCGGGTTATCGATAACGATTTctgcg                                                >  2:618105/3‑91 (MQ=255)
                                                    gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc                                           >  2:689935/1‑100 (MQ=255)
                                                     tttGATTTTGCTGCTGAAATCCGGGCGACTTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc                                          <  2:1623481/100‑1 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         <  1:109419/100‑1 (MQ=255)
                                                       tGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTg                                        >  1:2480672/1‑100 (MQ=255)
                                                            ttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgta                                   <  2:1659025/100‑1 (MQ=255)
                                                             tgctgtTGAAATCCGGGCGACCTAATGCAGCGATATACTGAGGTGCCAGTTCGAGCACTTTGGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaa                                  >  2:1417401/1‑100 (MQ=255)
                                                              gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag                                 <  1:657874/100‑1 (MQ=255)
                                                               ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                <  1:848995/100‑1 (MQ=255)
                                                                tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta                               >  1:505252/1‑100 (MQ=255)
                                                                   tgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAAt                             >  1:1505839/1‑99 (MQ=255)
                                                                   tgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATc                            >  2:363728/1‑100 (MQ=255)
                                                                    gAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcg                           >  1:1277495/1‑100 (MQ=255)
                                                                     aaaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgc                          >  2:955231/1‑100 (MQ=255)
                                                                      aaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgcg                         >  1:1691240/1‑100 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGCTTTCGCCGATGTCCTGACGTAAGTAATcgcg                         >  2:331833/1‑99 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        <  2:1289594/100‑1 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        >  1:14532/1‑100 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        <  2:633838/100‑1 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        <  1:1848823/100‑1 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGGTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        >  1:900849/1‑100 (MQ=255)
                                                                         ccGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGaa                      <  1:1195763/100‑1 (MQ=255)
                                                                          cGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAAt                     <  1:689939/100‑1 (MQ=255)
                                                                           gggCGACCTAATGCAGCGATGTACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATg                    <  2:291099/100‑1 (MQ=255)
                                                                           gggCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATg                    <  2:2251214/100‑1 (MQ=255)
                                                                             gCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                  <  1:1867614/100‑1 (MQ=255)
                                                                                 ccTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGaa              <  2:538607/100‑1 (MQ=255)
                                                                                  cTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAAc             >  2:225550/1‑100 (MQ=255)
                                                                                  cTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAAc             <  1:2533690/100‑1 (MQ=255)
                                                                                      tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  1:2474934/1‑100 (MQ=255)
                                                                                      tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  1:750687/1‑100 (MQ=255)
                                                                                       gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa        <  2:527586/100‑1 (MQ=255)
                                                                                       gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa        >  2:1919470/1‑100 (MQ=255)
                                                                                         aGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACg      <  1:1603928/100‑1 (MQ=255)
                                                                                             atatACTGACGTGCCAGTTCGAGCACTTTCGTGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  2:2084635/100‑1 (MQ=255)
                                                                                             atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  2:366266/100‑1 (MQ=255)
                                                                                             atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  2:2351/100‑1 (MQ=255)
                                                                                                 |                                                                                               
ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143544‑1143736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: