| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,197,853 | T→C | 16.5% | N129D (AAT→GAT) | ymfE ← | e14 prophage; putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,197,853 | 0 | T | C | 16.5% | 179.5 / 26.2 | 78 | N129D (AAT→GAT) | ymfE | e14 prophage; putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (27/38); new base C (3/10); total (30/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATAT > NC_000913/1197754‑1197950 | tCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGAtt < 1:997529/100‑1 (MQ=255) gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGc < 1:29586/100‑1 (MQ=255) aaaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTc < 1:371969/100‑1 (MQ=255) aTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCAt < 2:1314004/100‑1 (MQ=255) cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc < 2:1606056/100‑1 (MQ=255) cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc < 1:1667504/100‑1 (MQ=255) aaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACAt < 1:631188/100‑1 (MQ=255) aaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATtg < 1:941650/100‑1 (MQ=255) atatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATtgtg < 2:136423/100‑1 (MQ=255) aaaaaaaaaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATtgtg < 2:1127823/94‑1 (MQ=255) tatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGa < 1:1067066/100‑1 (MQ=255) atCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAg < 2:781605/100‑1 (MQ=255) atCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAg < 1:1021881/100‑1 (MQ=255) cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCtt > 1:1615095/1‑100 (MQ=255) aaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATc < 1:1373337/100‑1 (MQ=255) gTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCttta < 2:994169/100‑1 (MQ=255) tataCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtttat < 2:1447070/100‑1 (MQ=255) ataCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttatt < 1:1191735/100‑1 (MQ=255) cTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtttattta > 1:157170/1‑99 (MQ=255) tATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAAttt < 1:2054149/100‑1 (MQ=255) ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg > 2:1254138/1‑100 (MQ=255) ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg < 1:149937/100‑1 (MQ=255) cAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGt < 2:2037523/100‑1 (MQ=255) cAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCGTTACATTGTGAGCTTCATCTTTATTTAATTTTCTGt > 1:1828727/1‑100 (MQ=255) agaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGtt < 2:1808061/100‑1 (MQ=255) gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg > 1:369342/1‑100 (MQ=255) agagTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGAc > 1:796640/1‑100 (MQ=255) agagTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGAc < 1:63439/100‑1 (MQ=255) agagTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCTCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg < 2:2460237/98‑1 (MQ=255) aattCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTc < 1:342810/98‑1 (MQ=255) gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc < 1:2238817/100‑1 (MQ=255) gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc > 1:1997194/1‑100 (MQ=255) gTTCAATATAATGTTCGTCTTCAATTTTTCTTACTTCAGAGTAATATAGATTGCTAATTACATTGTGAGCTTCATATTTATTTAATTTTCTTTTGACTcc > 2:1809320/1‑100 (MQ=255) tCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAg > 1:81297/1‑100 (MQ=255) cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc > 1:1089404/1‑100 (MQ=255) cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc > 1:730241/1‑100 (MQ=255) cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc < 1:1283877/100‑1 (MQ=255) aaTATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGct > 2:2487034/1‑100 (MQ=255) atatAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc < 2:1574759/98‑1 (MQ=255) ataATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCtctc < 1:84718/100‑1 (MQ=255) ataATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAACTTTCTGTTGACTCCAGCtctc > 2:225073/1‑100 (MQ=255) taATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTcc > 1:1298957/1‑100 (MQ=255) aaTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCg < 1:1630464/100‑1 (MQ=255) aTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCCCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGt < 1:1928801/100‑1 (MQ=255) tGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTg < 2:157168/100‑1 (MQ=255) gTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATTGATTGCTCATTACATTGTGAGATTCATCTTTATTTAATTTTCTGTTGACTCCAGTTCTCCGt < 2:998999/98‑1 (MQ=255) tGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATaa < 1:2256014/100‑1 (MQ=255) tGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATaa < 2:1221495/100‑1 (MQ=255) cTTCAATTTTTTTTTTTTTTTGTTAATATAGATTGCTCATTACATTTTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGAt > 2:2420014/1‑95 (MQ=255) cTTCAATTTTTCTTACTTTAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg < 1:382345/100‑1 (MQ=255) cTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg > 1:2214132/1‑100 (MQ=255) cTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg < 1:387902/100‑1 (MQ=255) cTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg > 1:1876212/1‑100 (MQ=255) cTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg > 1:294960/1‑100 (MQ=255) ttCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGt > 2:1765206/1‑100 (MQ=255) tCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGtt > 1:335806/1‑100 (MQ=255) aaTTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGtttt < 2:2435939/100‑1 (MQ=255) aTTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTta < 1:2582157/100‑1 (MQ=255) ttttCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtata > 2:1649547/1‑100 (MQ=255) tttCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATaa < 1:2431538/100‑1 (MQ=255) tttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATaa < 2:2361335/100‑1 (MQ=255) cTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAAtt > 1:238290/1‑99 (MQ=255) cTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa < 1:2157999/100‑1 (MQ=255) cTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATg > 1:2364050/1‑100 (MQ=255) cTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATg > 1:1249702/1‑100 (MQ=255) ttCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGc < 2:2262207/100‑1 (MQ=255) aGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTa < 2:369338/100‑1 (MQ=255) aGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTa < 1:32810/100‑1 (MQ=255) aGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTa < 2:347064/100‑1 (MQ=255) aGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTa < 1:565385/100‑1 (MQ=255) gggTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTAt > 1:372279/1‑100 (MQ=255) gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTATATGCTTAt > 2:628879/1‑100 (MQ=255) ggTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATc < 1:2147012/100‑1 (MQ=255) ggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATc < 2:1933897/100‑1 (MQ=255) atatAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaa > 1:1941331/1‑100 (MQ=255) ataGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg < 1:48134/100‑1 (MQ=255) aGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGat > 1:745813/1‑100 (MQ=255) gATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGata > 2:24179/1‑100 (MQ=255) gATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGata > 2:2334118/1‑100 (MQ=255) ttGCTCATTAGATTGTGAGCTTCATCTTTATTTAATGTTCTGTTGACTGCAGCTCTGGGTGGTAATGGTGTTGTGATGAGATGTTTTTCTCAAAGatat > 2:2170695/1‑99 (MQ=255) | TCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATAT > NC_000913/1197754‑1197950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |