| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 236,569 | C→T | 25.4% | A168V (GCC→GTC) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 236,569 | 0 | C | T | 25.4% | 125.0 / 44.3 | 75 | A168V (GCC→GTC) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/32); new base T (9/10); total (33/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01 | |||||||||||
GGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAAC > NC_000913/236470‑236668 | ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc < 2:971476/100‑1 (MQ=255)ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc > 2:290075/1‑100 (MQ=255)ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc < 1:929375/100‑1 (MQ=255) aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGccc < 2:2048468/100‑1 (MQ=255) aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCa < 1:2432469/100‑1 (MQ=255) aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCa < 2:2540237/100‑1 (MQ=255) aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCa > 2:209462/1‑100 (MQ=255) aaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAg > 1:671762/1‑100 (MQ=255) aTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTTAACGAACGCTGCACGGGGCATTACTCGATGCCCAGa < 1:1834020/100‑1 (MQ=255) tGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCa > 1:1115829/1‑97 (MQ=255) cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCtt > 2:999333/1‑99 (MQ=255) ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGc > 2:2669/1‑100 (MQ=255) ccGGTAAGCGCAACAGCCTCGATGCGTTATGTACTCGCGACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGc < 1:533288/100‑1 (MQ=255) cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg > 1:1673853/1‑100 (MQ=255) cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg < 1:1679715/100‑1 (MQ=255) ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCgg < 2:2602870/100‑1 (MQ=255) ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCCCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGc < 2:309001/98‑1 (MQ=255) gTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGa > 1:1454335/1‑100 (MQ=255) aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt > 2:1490313/1‑100 (MQ=255) aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt < 1:129339/100‑1 (MQ=255) gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt < 1:1625457/100‑1 (MQ=255) cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGACCGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGttt < 2:414214/100‑1 (MQ=255) cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGttt < 1:690953/100‑1 (MQ=255) cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt < 2:1867707/99‑1 (MQ=255) cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGAATTACTCGATGCCCAGATCCTTGCGGAAGttt > 2:1435974/1‑100 (MQ=255) gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGttt > 1:770332/1‑99 (MQ=255) cAACAGCCTCGATGCGTTATGTGCTCGCTCCGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTTCTCGATGTCCAGATCCTTGCGGAAGTTTaa < 1:999338/100‑2 (MQ=255) aCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCt < 2:730979/100‑1 (MQ=255) gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc > 1:1261216/1‑100 (MQ=255) gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc > 2:1065034/1‑100 (MQ=255) gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGc > 1:331268/1‑100 (MQ=255) ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg < 2:85927/100‑1 (MQ=255) ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg > 2:1056439/1‑100 (MQ=255) cTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGa < 1:389701/100‑1 (MQ=255) tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGAt < 1:2456978/100‑1 (MQ=255) cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATg > 1:2424163/1‑100 (MQ=255) gATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGACATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATg > 2:796557/1‑99 (MQ=255) cGTTATGTGCTCTCTACGAAATAGATAACAGTAAACAAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCgg < 1:1082811/100‑1 (MQ=255) cGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCgg > 1:1120348/1‑100 (MQ=255) gTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt > 2:146285/1‑100 (MQ=255) gTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt > 1:934210/1‑100 (MQ=255) tATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg < 1:2030787/100‑1 (MQ=255) aTGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtggt < 1:2539808/100‑1 (MQ=255) tgtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc < 1:15720/100‑1 (MQ=255) gCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaaa < 1:290078/100‑1 (MQ=255) tCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACg < 1:1651452/100‑1 (MQ=255) tACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGa < 1:636639/100‑1 (MQ=255) aCGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGa > 1:629118/1‑99 (MQ=255) cGAAATAGATAACAGTAAACGAACGCTAAAAGGGGCATTACTCGATGTCCAGATCCTTGAGGAAGTTTATCTGACGATGACCAGTGGTCAAACGTCGATg > 2:2593686/1‑100 (MQ=255) gATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg < 2:1747351/100‑1 (MQ=255) gATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg < 1:1144778/100‑1 (MQ=255) aTAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGc < 2:2267132/100‑1 (MQ=255) tAACAGTAAACGAACTCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTCCg < 2:924656/100‑1 (MQ=255) tAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg > 2:2045676/1‑100 (MQ=255) aaCAGTAAACGGGTTCTGCACGGGGCATTACTCGATGTCCGGATCTTTGCGGAAGTTTATCTGGCGATGACCGGTGGTTAAACGTCGATGGCTTTTGCGa < 2:1978292/100‑1 (MQ=255) aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt < 2:2478318/100‑1 (MQ=255) cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg > 2:2371928/1‑100 (MQ=255) aGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg < 1:209464/100‑1 (MQ=255) gTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGa > 1:2381904/1‑100 (MQ=255) tAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGaa > 1:2451680/1‑100 (MQ=255) acgCTGCACGGGGCATTACTCGATGCCCAGATCCTGGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgaga < 2:2446995/98‑1 (MQ=255) cgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGac < 2:2245563/100‑1 (MQ=255) cTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGacac > 1:2102673/1‑100 (MQ=255) tGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGAcaca > 2:394463/1‑100 (MQ=255) tGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGAcaca < 1:304655/100‑1 (MQ=255) tGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGAcaca < 2:1804550/100‑1 (MQ=255) gCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaa > 2:1475672/1‑100 (MQ=255) aCGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaaca < 2:970108/100‑1 (MQ=255) aCGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaaca > 2:2373286/1‑100 (MQ=255) cGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAg < 2:1770675/100‑1 (MQ=255) tACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAGACGTCGATGGCTTTTGCGATGGAAGGAGTGACACAACAGCAACAAgg > 2:2421720/1‑100 (MQ=255) tACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAgg > 2:2421728/1‑100 (MQ=255) tACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAgg < 1:1537925/100‑1 (MQ=255) tACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAgg < 1:170959/100‑1 (MQ=255) aCTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGt > 2:621441/1‑100 (MQ=255) aCTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGt > 1:77038/1‑100 (MQ=255) gATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAg < 2:251412/100‑1 (MQ=255) aTGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGc < 2:134371/100‑1 (MQ=255) aTGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGc < 1:1445155/100‑1 (MQ=255) cccAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGcaac > 1:1828521/1‑100 (MQ=255) cccAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGcaac > 1:1730093/1‑100 (MQ=255) cccAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGcaac > 2:2045439/1‑100 (MQ=255) | GGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAAC > NC_000913/236470‑236668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |