| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,533,713 | A→C | 16.0% | F47V (TTT→GTT) | yddH ← | flavin reductase like‑protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,533,713 | 0 | A | C | 16.0% | 147.0 / 30.3 | 69 | F47V (TTT→GTT) | yddH | flavin reductase like‑protein |
| Reads supporting (aligned to +/- strand): ref base A (29/29); new base C (5/6); total (34/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-01 | |||||||||||
AACGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGA > NC_000913/1533615‑1533810 | aaCGCCCGGGATAACGATGCCAAATTTACCGTTATGTTTAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAAc < 1:878624/100‑1 (MQ=255) cGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTc > 1:1324453/1‑100 (MQ=255) gCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTcc < 2:1328699/100‑1 (MQ=255) cGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcg < 1:477285/100‑2 (MQ=255) gggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCg < 1:2551064/100‑1 (MQ=255) ggATAACGATGCCAAATTTACCGTTGTGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcg < 2:1881141/100‑4 (MQ=255) ggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcg < 1:94072/100‑4 (MQ=255) ggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcg < 1:255842/100‑4 (MQ=255) gATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGc > 1:1222232/1‑100 (MQ=255) aTAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcgtg < 1:2528072/100‑6 (MQ=255) tAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCAt < 2:1896261/100‑1 (MQ=255) tAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCAt > 1:2019194/1‑100 (MQ=255) aaCGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcgtggc < 1:1833868/100‑8 (MQ=38) aCGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTg < 1:458756/100‑1 (MQ=255) cGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGa > 1:1979073/1‑100 (MQ=255) gATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGAc < 2:421252/100‑1 (MQ=255) gCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAg < 2:1215178/100‑1 (MQ=255) ccAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAgg > 1:1739461/1‑100 (MQ=255) ccAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAgg > 1:111478/1‑100 (MQ=255) cAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGc < 2:1294151/100‑1 (MQ=255) aaTTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTg < 2:1380019/100‑1 (MQ=255) aTTTACCGTTATGTTCAATCCACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGc < 2:1062002/100‑1 (MQ=255) tACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAgt < 2:2153340/100‑2 (MQ=255) ccGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCa > 1:1061207/1‑100 (MQ=255) ttCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTa < 1:1478615/100‑1 (MQ=255) tcaatcaaCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTAc < 1:319514/100‑1 (MQ=255) atcaaCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTAcgcc > 1:2570940/1‑100 (MQ=255) aaCTGCCTGGTCCATGTCGATTTAGCTACTACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCGTAATGTTAcgac > 2:1771570/1‑95 (MQ=255) cTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGATGCAGCCATAATGTTACGCCGCTgg > 1:629883/1‑100 (MQ=255) cTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCAATGACCAGGCTGCAGCCATAATGTTACGCCGCTgg > 2:1375303/1‑100 (MQ=255) tCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTggg > 2:948461/1‑100 (MQ=255) cccTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCCCCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGa > 2:1495038/1‑100 (MQ=255) cccTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGa > 1:482432/1‑100 (MQ=255) ggTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGc > 2:1971225/1‑100 (MQ=255) ggTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGc < 1:2196096/100‑1 (MQ=255) gTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCCAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCt > 1:1897580/1‑100 (MQ=255) gTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCt < 2:2155701/100‑1 (MQ=255) ccATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCa < 2:1691747/100‑1 (MQ=255) aTGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATc < 2:103251/100‑1 (MQ=255) aTGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATc < 2:103298/100‑1 (MQ=255) aTGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATc > 1:2560277/1‑100 (MQ=255) tGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCg > 1:408107/1‑100 (MQ=255) tGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCg < 2:331906/100‑1 (MQ=255) gTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGa < 1:948466/100‑1 (MQ=255) gTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGa > 2:286881/1‑100 (MQ=255) gTCGATTTATCTACCACAATAGCCAAGCGTGGAGGTTCAAACTCCACCGGCAATGACCCGGCTGCAGCCATAATGTTACGCCGCTGGGATCGCTCATCGa > 2:496186/1‑100 (MQ=255) cGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGaaa < 1:392154/100‑1 (MQ=255) cGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGaaa < 2:2430047/100‑1 (MQ=255) cTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGAt > 1:1128915/1‑100 (MQ=255) cTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGAt < 1:1461181/100‑1 (MQ=255) tACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATc > 1:202210/1‑100 (MQ=255) ccACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCaa > 1:1463207/1‑100 (MQ=255) cacaATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAg < 2:573103/100‑1 (MQ=255) acaATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGa > 1:1707243/1‑100 (MQ=255) aTCGCCACGCGTGGAGGTTCAGACTCGCCCGGCATTGACCAGGCTGCAGCCATAATGTTCCGCCGCTGGGATTGCTCATCGAATCTGGTGATCAAGACCg < 2:2369522/100‑1 (MQ=255) cGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGtt > 2:1498199/1‑100 (MQ=255) gCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTg > 1:162021/1‑100 (MQ=255) gCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTg > 1:1980086/1‑100 (MQ=255) ccACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTgg < 1:2304521/100‑1 (MQ=255) gccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGc > 1:289511/5‑100 (MQ=255) gccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGc > 1:2121875/5‑100 (MQ=255) gccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGc > 1:802777/5‑100 (MQ=255) aCGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGc < 1:701127/100‑1 (MQ=255) ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc > 2:2089819/4‑100 (MQ=255) ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc > 2:2167822/4‑100 (MQ=255) gTGGAGGTTCAAACTCCACCGGCAGTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATg < 2:1635600/100‑1 (MQ=255) tGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGa > 1:1132998/1‑100 (MQ=255) tGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGa < 1:1657172/100‑1 (MQ=255) ggAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGAt > 2:1900464/1‑100 (MQ=255) aaaCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGa > 1:2229702/1‑100 (MQ=255) | AACGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGA > NC_000913/1533615‑1533810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |