Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,755,639	G→A	100%	intergenic (‑498/‑59)	ydhZ ← / → pykF	uncharacterized protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,639	0	G	A	100.0%	224.2 / NA	68	intergenic (‑498/‑59)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/29);  total (39/29)

CTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCG  >  NC_000913/1755542‑1755733                                                                                                  |                                                                                               cTGAGACACAAGCACACATTCCTCTGCACGATTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACa                                                                                              >  1:1039454/1‑100 (MQ=255)   gagaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGt                                                                                            >  1:1022198/1‑100 (MQ=255)    agaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTg                                                                                           <  1:551965/100‑1 (MQ=255)    agaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTg                                                                                           <  1:2098033/100‑1 (MQ=255)     gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                          <  1:1508804/100‑1 (MQ=255)       cacaAGCACACATTCCTCTGTACGCTTTTTCTATGTCACGTATCCTTAGGTCGTGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcg                                                                                         >  2:1164001/1‑99 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTTTTTTTTTTTTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                        >  1:398313/1‑100 (MQ=255)        acacGCACACATCCCTCTGCTCGCCTTTTCGAGGTCACCTATCCTTAGACCGAGGCACCACCACTTTCGTAATACCGGAGTCGCTTTCCGACAGTGCGcc                                                                                       <  2:672428/100‑1 (MQ=255)        acaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGcc                                                                                       >  2:340041/1‑100 (MQ=255)           aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                    >  1:301372/1‑100 (MQ=255)             cacacaTTCCTCTGCACGCTTTTTCGTTGTCACCTATCCTTAGAGCGATGCACGACCCATTTCGTACTACCGATTTAGCTTTCCGACAATgagc                                                                                        >  2:440242/1‑94 (MQ=255)             cacacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGaa                                                                                  >  1:163604/1‑100 (MQ=255)              acacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGaaa                                                                                 <  1:349377/100‑1 (MQ=255)                 caTTCCTCTGCACGCTTTTTCGATGTCACCTAACCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCa                                                                              >  1:2470258/1‑100 (MQ=255)                   ttCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAg                                                                            <  2:1334829/100‑1 (MQ=255)                    tCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGt                                                                           >  1:947026/1‑100 (MQ=255)                      ctctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGttt                                                                         >  2:2570769/1‑100 (MQ=255)                         tGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtctc                                                                      <  2:2134396/100‑1 (MQ=255)                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                     <  2:957369/100‑1 (MQ=255)                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                     >  1:267369/1‑100 (MQ=255)                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                     >  1:2322582/1‑100 (MQ=255)                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                     <  1:1478877/100‑1 (MQ=255)                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCCAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                     <  2:1248269/100‑1 (MQ=255)                           tgtgctcttccGATCTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                    <  1:2249450/90‑1 (MQ=255)                           cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                    >  1:5501/1‑100 (MQ=255)                                   ttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtct                                                            <  1:248662/100‑1 (MQ=255)                                   ttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtct                                                            >  2:1946881/1‑100 (MQ=255)                                    tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtctc                                                           >  2:2453692/1‑100 (MQ=255)                                     cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                          <  2:2136700/100‑1 (MQ=255)                                       aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                        <  1:563087/100‑1 (MQ=255)                                         gTCACCTATCCTTAGAGCGAGGCACCACCACTCTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACtt                                                      >  1:2467043/1‑100 (MQ=255)                                           cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                    >  2:955812/1‑100 (MQ=255)                                           cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                    >  1:717086/1‑100 (MQ=255)                                             ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                  >  2:853772/1‑100 (MQ=255)                                               tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                >  2:1806180/1‑100 (MQ=255)                                               tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                >  2:1855954/1‑100 (MQ=255)                                               tATCCTTAAAGCTACGCCCCACCACGTTCGTAATCCCGGATTCGCTTTCCGACAGTCCGCTCAGAAAGCAAGGTTCCCCCATCCTTCACAACTTaaa                                                   <  2:535419/97‑1 (MQ=255)                                                   cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                            <  2:2303602/100‑1 (MQ=255)                                                   cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                            >  1:1888088/1‑100 (MQ=255)                                                    ttAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaaga                                           >  2:1239377/1‑100 (MQ=255)                                                     tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagac                                          <  1:1239382/100‑1 (MQ=255)                                                       gcgCGAGGCAACACCACTTTCGTAATACCGGCTTCGCTTTCCGACAGTGCGCCCAGCAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg                                        <  1:2475461/98‑1 (MQ=255)                                                       gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg                                        <  2:209320/100‑1 (MQ=255)                                                       gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg                                        <  1:2113875/100‑1 (MQ=255)                                                         gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTc                                      >  2:582702/1‑100 (MQ=255)                                                         gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTc                                      >  2:65316/1‑100 (MQ=255)                                                             ggCACCACCACTTTCGTAATACGGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                  >  2:1513040/1‑100 (MQ=255)                                                             ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                  >  1:642746/1‑100 (MQ=255)                                                             ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                  >  1:1342780/1‑100 (MQ=255)                                                             ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                  <  1:493241/100‑1 (MQ=255)                                                                 ccaccacTTTCGTAATACCGGATTCTCTTTCCGACAGTGCGCCCAGAAAGCCATTTTCTCCCATCCTCCTCAACTTAAAGACTAACACTGTCATGaaaa                               <  2:1614390/99‑1 (MQ=255)                                                                 ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaa                              >  1:852522/1‑100 (MQ=255)                                                                  caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                             >  2:2480246/1‑100 (MQ=255)                                                                        tttCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaa                       <  2:820689/100‑1 (MQ=255)                                                                          tCGTAATACCGGATTCGCTTTCCGACAGTGCGCCGAGAAAGCAAGTTTATCCTATCCTTCTCAACTTAAAGAGTAAGCATGTCATGAAAAAGATCAAAAt                     >  2:796581/1‑100 (MQ=255)                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                <  1:2160027/100‑1 (MQ=255)                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCACATCCTTCTCAACTTACAGCCTAAGACTGTCATGAAAAAGACCAAAATTGttt                >  2:1285575/1‑100 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAATGTTTg               >  2:2169800/1‑100 (MQ=255)                                                                                 aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc              <  1:287624/100‑1 (MQ=255)                                                                                    ggATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAcc           <  2:164471/100‑1 (MQ=255)                                                                                     gATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCa          >  1:203007/1‑100 (MQ=255)                                                                                       ttCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAGTGTTTGCACCATc        <  2:1613008/100‑1 (MQ=255)                                                                                        tCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCg       <  2:925274/100‑1 (MQ=255)                                                                                         cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg      >  1:1976188/1‑100 (MQ=255)                                                                                           cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc    >  2:268056/1‑100 (MQ=255)                                                                                           cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc    >  1:1677953/1‑100 (MQ=255)                                                                                             ttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCg  >  2:128731/1‑100 (MQ=255)                                                                                             ttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCg  <  1:2570782/100‑1 (MQ=255)                                                                                                  |                                                                                               CTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCG  >  NC_000913/1755542‑1755733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑