Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,868,250	A→G	100%	Y448C (TAC→TGC)	yeaG →	protein kinase, endogenous substrate unidentified; autokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,868,250	0	A	G	100.0%	235.7 / NA	67	Y448C (TAC→TGC)	yeaG	protein kinase, endogenous substrate unidentified; autokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/33);  total (34/33)

GAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATAT  >  NC_000913/1868155‑1868344                                                                                                |                                                                                               gacccgTCTGTCGACGCGTTTTGCGTTTAAGTCCCTCTACATCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCCTCTGTTCTGCGt                                                                                             <  2:1823620/94‑1 (MQ=255)    cGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTg                                                                                         >  1:540858/1‑100 (MQ=255)    cGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTg                                                                                         <  1:2031164/100‑1 (MQ=255)    cGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTg                                                                                         <  2:1950420/100‑1 (MQ=255)    cGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTg                                                                                         >  1:1559289/1‑100 (MQ=255)        cTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc                                                                                     <  2:1009951/100‑1 (MQ=255)        cTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc                                                                                     <  2:1318171/100‑1 (MQ=255)         tGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAca                                                                                    <  1:1299535/100‑1 (MQ=255)             gACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcagcag                                                                                <  2:652791/100‑1 (MQ=255)             gACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcagcag                                                                                >  1:1607128/1‑100 (MQ=255)                gcgTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAtt                                                                             >  1:1054264/1‑100 (MQ=255)                gcgTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAtt                                                                             <  1:1555425/100‑1 (MQ=255)                    tttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgc                                                                         >  1:1444624/1‑100 (MQ=255)                    tttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgc                                                                         <  1:735418/100‑1 (MQ=255)                      tGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgcgc                                                                       >  2:101016/1‑100 (MQ=255)                       gCGTTTAAGATCCTCTCCCGCGTGTTCAACGTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgc                                                                         <  1:125125/97‑1 (MQ=255)                        cGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGATCTGCGTCCTGGAACAGCAGATTGAGCGCGa                                                                     >  2:473526/1‑100 (MQ=255)                         gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg                                                                    >  2:20213/1‑100 (MQ=255)                         gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg                                                                    >  1:354542/1‑100 (MQ=255)                           ttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCa                                                                  >  1:13420/1‑100 (MQ=255)                           ttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCa                                                                  <  2:2176038/100‑1 (MQ=255)                              aGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACCGCAGATTGAGCGCGAGCAGtt                                                               >  2:2396686/1‑100 (MQ=255)                              aGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGtt                                                               >  1:1094003/1‑100 (MQ=255)                                aTCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTcc                                                             <  2:811093/100‑1 (MQ=255)                                  ccTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCca                                                           >  1:1961764/1‑100 (MQ=255)                                   ctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcac                                                          <  2:1949691/100‑1 (MQ=255)                                    tctcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcaca                                                         <  2:1874525/100‑1 (MQ=255)                                    tctcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcaca                                                         <  1:101017/100‑1 (MQ=255)                                      tcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAag                                                       <  2:1732548/100‑1 (MQ=255)                                       cccGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAaga                                                      >  2:1701026/1‑100 (MQ=255)                                       cccGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAaga                                                      <  1:1293280/100‑1 (MQ=255)                                        ccGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAagag                                                     >  1:1109016/1‑100 (MQ=255)                                         cgcgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgc                                                    >  1:693608/1‑100 (MQ=255)                                           cgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcag                                                  <  1:2103223/100‑1 (MQ=255)                                           cgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAAACCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcag                                                  <  1:1824462/100‑1 (MQ=255)                                             tgtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggc                                                <  1:239319/100‑1 (MQ=255)                                              gtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggca                                               >  2:2603152/1‑100 (MQ=255)                                                  aaCTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAAc                                           >  1:2463563/1‑100 (MQ=255)                                                    cTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGc                                         >  1:2489230/1‑100 (MQ=255)                                                      tCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTa                                       <  2:794756/100‑1 (MQ=255)                                                      tCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTa                                       <  1:1771462/100‑1 (MQ=255)                                                        gATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTAt                                      >  2:1666741/1‑99 (MQ=255)                                                          tCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTg                                   >  2:1238204/1‑100 (MQ=255)                                                            aTGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGa                                 <  2:2391285/100‑1 (MQ=255)                                                            aTGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGa                                 <  1:362747/100‑1 (MQ=255)                                                            aTGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGa                                 <  1:2154283/100‑1 (MQ=255)                                                             tGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                >  2:2026059/1‑100 (MQ=255)                                                               tAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGtt                              <  1:1364274/100‑1 (MQ=255)                                                                    gTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGa                         >  1:805900/1‑100 (MQ=255)                                                                      ggCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGaaa                       >  2:837062/1‑100 (MQ=255)                                                                       gcagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAg                      <  2:1683483/100‑1 (MQ=255)                                                                       gcagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAg                      <  1:581540/100‑1 (MQ=255)                                                                        cagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                     <  2:1093998/100‑1 (MQ=255)                                                                         agcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGt                    >  2:1590001/1‑100 (MQ=255)                                                                          gcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGtt                   >  2:86962/1‑100 (MQ=255)                                                                             aaCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATc                <  1:1705542/100‑1 (MQ=255)                                                                              aCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGGGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCt               <  2:1663074/100‑1 (MQ=255)                                                                              aCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCt               >  1:946462/1‑100 (MQ=255)                                                                              aCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCt               >  1:945480/1‑100 (MQ=255)                                                                              aCCCGGTCCATCTGCTCTGCGTCCTAGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCt               <  2:1770597/100‑1 (MQ=255)                                                                               cccGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTg              >  2:1314469/1‑100 (MQ=255)                                                                                       aTCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGaa      >  1:2344038/1‑100 (MQ=255)                                                                                        tCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGaaa     >  2:1762854/1‑100 (MQ=255)                                                                                         cTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAat    >  1:1890271/1‑100 (MQ=255)                                                                                          tGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAata   <  1:2163878/100‑1 (MQ=255)                                                                                           gTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAatat  >  2:2256704/1‑100 (MQ=255)                                                                                           gTTCTGCGTCCTGGAACAGCAGATTGAGCACGAGCAGTTCCCACAAGAGCAGGCCGAACGCTATCTGGAGATCCTGAAAGGTTATCTGATCCCGACatat  >  2:2125574/1‑100 (MQ=255)                                                                                                |                                                                                               GAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATAT  >  NC_000913/1868155‑1868344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑