Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,270,605 G→A 16.4% intergenic (+60/‑121) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,270,6050GA16.4% 229.4 / 26.0 94intergenic (+60/‑121)mepS/rtnmurein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (40/38);  new base A (7/9);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAA  >  NC_000913/2270507‑2270704
                                                                                                  |                                                                                                   
cGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAgg                                                                                                    <  2:37691/100‑1 (MQ=255)
 gTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGc                                                                                                   <  1:397175/100‑1 (MQ=255)
 gTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGc                                                                                                   <  2:827219/100‑1 (MQ=255)
     cAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTg                                                                                               >  1:189928/1‑100 (MQ=255)
     cAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTg                                                                                               >  2:2207183/1‑100 (MQ=255)
     cAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTg                                                                                               <  2:894994/100‑1 (MQ=255)
       aCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCt                                                                                             <  2:2103138/100‑1 (MQ=255)
       aCGAAGCACGCCGGGTTCTCAGCCGCAGCTAAAAAACCGATTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCt                                                                                             <  2:1828326/100‑1 (MQ=255)
       aCGAAGCACGCCGGGGTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGGTAACTGAAAGCACTGCTTAGGCAGTGCt                                                                                             >  2:851019/1‑100 (MQ=255)
          aaGCAGGCCGGGTTCTCAGCCGCACGTAATAAACCGGTTGGATGCAATGTCTTGGCTAGCCTGACTAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttc                                                                                          <  2:1050395/100‑2 (MQ=255)
          aaGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttc                                                                                          <  1:490151/100‑2 (MQ=255)
            gCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttcag                                                                                        <  2:1958053/100‑4 (MQ=39)
            gCACGCCGGGTTATCAGCTGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGTTATCCTGACGAGTTAACTTAAAGCACTGCCTAAGCAGTGCtttcag                                                                                        <  1:1276195/100‑4 (MQ=37)
            gCACGCCGGGTGCTCAGCCGCAGCTAATGAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttcag                                                                                        <  2:2016627/100‑4 (MQ=38)
             cACGCCGGGTTCTCAGCCGCAGCTAATAACCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTg                                                                                       <  2:1556394/100‑1 (MQ=255)
             cACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTg                                                                                       <  2:255820/100‑1 (MQ=255)
             cACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttcagt                                                                                       <  1:11705/100‑5 (MQ=39)
              aCGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGACAGCACTGCTTAGGCAGTGCTTTTTTGt                                                                                      >  2:2458034/1‑100 (MQ=255)
               cGCCGGGTTCTCAGCCGCAGCAAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtt                                                                                            <  2:305950/93‑1 (MQ=255)
                 ccGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGtttt                                                                                   <  2:354078/100‑1 (MQ=255)
                        ctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTtcattcat                                                                            >  1:416130/1‑100 (MQ=255)
                         tcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                           <  2:1915455/100‑1 (MQ=255)
                         tcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                           <  1:2185915/100‑1 (MQ=255)
                         tcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                           <  2:1973889/100‑1 (MQ=255)
                           aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                           >  2:485938/1‑98 (MQ=255)
                           aGCCGCAGCTAATAAACAGTTTGGATGCAATCCCTCGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGTCAGTGCTTTTTTGTTTTCATTCATCag                                                                         >  2:487653/1‑100 (MQ=255)
                            gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCaga                                                                        >  2:2068836/1‑100 (MQ=255)
                                 aGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGaaaa                                                                   >  1:1683319/1‑100 (MQ=255)
                                  gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAat                                                                  <  1:723312/100‑1 (MQ=255)
                                  gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAat                                                                  <  2:1437020/100‑1 (MQ=255)
                                  gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAat                                                                  <  1:1057127/100‑1 (MQ=255)
                                  gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAat                                                                  <  2:1435307/100‑1 (MQ=255)
                                   cTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAGatg                                                                 >  2:1463497/1‑100 (MQ=255)
                                   cTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAatg                                                                 >  2:919705/1‑100 (MQ=255)
                                     aataaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAatgat                                                               <  2:2112282/100‑1 (MQ=255)
                                     aataaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAatgat                                                               <  2:482410/100‑1 (MQ=255)
                                       taaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGt                                                             >  1:1166793/1‑100 (MQ=255)
                                           ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTcc                                                         <  1:483940/100‑1 (MQ=255)
                                            cGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg                                                        <  1:956232/100‑1 (MQ=255)
                                            cGTTTGCATGCAATCACTTGGCTAACCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTATTTTTTTTCATCACAAGAAATTATGttttag                                                        >  2:281140/1‑97 (MQ=255)
                                              tttGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgcg                                                      >  1:2020937/1‑100 (MQ=255)
                                              tttGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgcg                                                      <  2:2281808/100‑1 (MQ=255)
                                                tGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGt                                                     >  2:2024733/1‑99 (MQ=255)
                                                tGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTc                                                    >  1:2330336/1‑100 (MQ=255)
                                                tGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTc                                                    >  2:2500098/1‑100 (MQ=255)
                                                 ggATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCt                                                   <  1:2437543/100‑1 (MQ=255)
                                                  gATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCtt                                                  <  2:1039986/100‑1 (MQ=255)
                                                  gATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCtt                                                  >  2:2014393/1‑100 (MQ=255)
                                                   aTGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTg                                                 >  1:1095982/1‑100 (MQ=255)
                                                   aTGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTg                                                 >  2:1247030/1‑100 (MQ=255)
                                                    tGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGa                                                >  1:721841/1‑100 (MQ=255)
                                                    tGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGa                                                >  2:117936/1‑100 (MQ=255)
                                                      cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATc                                              >  1:698227/1‑100 (MQ=255)
                                                          cccTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAgg                                          <  2:352591/100‑1 (MQ=255)
                                                           ccTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGc                                         <  2:2000974/100‑1 (MQ=255)
                                                               ggCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCtata                                     >  1:2049443/1‑100 (MQ=255)
                                                                 cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTTTTTTTTTTTTTCAGAGAAAAGGATGTTTCCGCGTTTTGATCTATGCtgttg                                    >  2:1911487/1‑95 (MQ=255)
                                                                   aTCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTcc                                 <  2:1512370/100‑1 (MQ=255)
                                                                   aTCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTcc                                 >  1:166857/1‑100 (MQ=255)
                                                                      cTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCgg                               >  2:803655/1‑99 (MQ=255)
                                                                       tGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTc                             <  1:324564/100‑1 (MQ=255)
                                                                        gACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCa                            >  1:2244653/1‑100 (MQ=255)
                                                                         aCGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCAt                           <  1:1310276/100‑1 (MQ=255)
                                                                           gAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTg                         >  1:634907/1‑100 (MQ=255)
                                                                           gAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTg                         <  1:1091751/100‑1 (MQ=255)
                                                                            aGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGt                        <  2:1273453/100‑1 (MQ=255)
                                                                              ttAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTa                      <  2:320121/100‑1 (MQ=255)
                                                                              ttAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTa                      <  1:472683/100‑1 (MQ=255)
                                                                              ttAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTa                      <  1:658981/100‑1 (MQ=255)
                                                                              ttAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTa                      <  2:1095977/100‑1 (MQ=255)
                                                                                acaaaaaaGCACTGCCTCAGCCGTGCTTTTTTGTTTTCATTCATCAGAGCAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATc                    >  2:2591827/6‑100 (MQ=255)
                                                                                aaCTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATc                    <  2:2259765/100‑1 (MQ=255)
                                                                                 caaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCTAGGCTATAGTCCGGTCATTGTTATCt                   >  2:946639/5‑100 (MQ=38)
                                                                                 caaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCt                   >  2:1897532/5‑100 (MQ=39)
                                                                                 caaaaaaGCACTGCCTAAGCAGTGCTTTCTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCt                   >  1:160938/5‑100 (MQ=38)
                                                                                 aCTGAAAGCACTGCTTAGGCAGTGCTTTTTTTTTTTTTTTTTTCAGAGAAAATGATGTTTCCGCGTCTTGTTTTAGGCTATAGTCTGGTCATTGtttttt                   >  2:2511612/1‑96 (MQ=255)
                                                                                 aCTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCt                   >  1:354813/1‑100 (MQ=255)
                                                                                  cTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCtt                  >  1:2492788/1‑100 (MQ=255)
                                                                                  aaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCtt                  >  1:2174475/4‑100 (MQ=39)
                                                                                   aaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCttt                 >  1:787454/3‑100 (MQ=255)
                                                                                    gAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCtttt                <  1:2458047/100‑1 (MQ=255)
                                                                                      aaGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTaa              >  1:2371310/1‑100 (MQ=255)
                                                                                       aGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTaaa             <  1:520535/100‑1 (MQ=255)
                                                                                        gCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAt            >  1:1820614/1‑100 (MQ=255)
                                                                                         cACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtg           <  2:2140821/100‑1 (MQ=255)
                                                                                          aCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgt          >  1:1915598/1‑100 (MQ=255)
                                                                                          aCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgt          >  1:613077/1‑100 (MQ=255)
                                                                                            tGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttg        <  1:2387688/100‑1 (MQ=255)
                                                                                            tGCTTAGGCAGTGCTTTTTTGTTTTCATTCATAAGAGAAAATGCTGTTTCCGAGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttg        >  2:577413/1‑100 (MQ=255)
                                                                                             gCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttgt       >  1:2052122/1‑100 (MQ=255)
                                                                                             gCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttgt       <  2:1915589/100‑1 (MQ=255)
                                                                                             gCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttgt       <  2:613074/100‑1 (MQ=255)
                                                                                              cTTAGGCAGTGCTTTTTTTTTTTTTTTTATAAGAGAAACTGATGTTTCCGCGTCTTGATTCAGTCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTc      >  2:1600980/1‑100 (MQ=255)
                                                                                               ttAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCg     >  1:2514731/1‑100 (MQ=255)
                                                                                               ttAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCg     >  2:2077533/1‑100 (MQ=255)
                                                                                                  ggCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTaa  >  1:594784/1‑100 (MQ=255)
                                                                                                  ggCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTaa  >  2:2335311/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAA  >  NC_000913/2270507‑2270704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: