Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,553,210 A→G 16.0% K287E (AAA→GAA)  amiA → N‑acetylmuramoyl‑l‑alanine amidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,553,2100AG16.0% 200.6 / 26.6 86K287E (AAA→GAA) amiAN‑acetylmuramoyl‑l‑alanine amidase I
Reads supporting (aligned to +/- strand):  ref base A (48/24);  new base G (7/7);  total (55/31)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

TGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGG  >  NC_000913/2553112‑2553307
                                                                                                  |                                                                                                 
tGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGaa                                                                                                  <  1:291979/100‑1 (MQ=255)
  ttAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGaaaa                                                                                                <  2:802464/100‑1 (MQ=255)
   tAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGaaaaa                                                                                               >  2:853472/1‑100 (MQ=255)
    aGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAg                                                                                              <  1:1307583/100‑1 (MQ=255)
     ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGc                                                                                             <  1:126939/100‑1 (MQ=255)
     ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGc                                                                                             <  2:1873306/100‑1 (MQ=255)
     ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCAGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGc                                                                                             >  2:1420286/1‑100 (MQ=255)
        aCGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGAt                                                                                          <  1:2203023/100‑1 (MQ=255)
           gcggcgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAg                                                                                       >  1:312294/1‑100 (MQ=255)
           gcggcgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGATATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAg                                                                                       >  2:655975/1‑100 (MQ=255)
              gcgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTa                                                                                    <  1:1821085/100‑1 (MQ=255)
                 tttCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGa                                                                                 >  2:1044249/1‑100 (MQ=255)
                 tttCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGa                                                                                 >  2:1297888/1‑100 (MQ=255)
                    cGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAAc                                                                              >  2:200670/1‑100 (MQ=255)
                    cGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAAc                                                                              >  2:2447633/1‑100 (MQ=255)
                     gTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAAcc                                                                             <  1:2369954/100‑1 (MQ=255)
                       cAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCg                                                                           >  1:1590092/1‑100 (MQ=255)
                       cAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCg                                                                           <  2:2197162/100‑1 (MQ=255)
                         gAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGAc                                                                         <  1:1682365/100‑1 (MQ=255)
                         gAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGAc                                                                         >  1:613060/1‑100 (MQ=255)
                         gAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGAc                                                                         >  1:1450892/1‑100 (MQ=255)
                         gAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGAc                                                                         >  1:1547275/1‑100 (MQ=255)
                             atgggcgccgCGATTGGTGAAGGCGTGTTCAGGTATAGCCCCCGGTGGGACACGCAGCATGAAAAATCGAAAAAGCGATAAGTTATGAAACCCGACGca                                                                      >  2:138873/10‑99 (MQ=255)
                              tCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGcaca                                                                    <  1:1569336/100‑1 (MQ=255)
                                gCCACAGCGATTGCTGAAGGCGGGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACAcc                                                                  >  1:2533317/1‑100 (MQ=255)
                                  cacaGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAg                                                                <  1:1394770/100‑1 (MQ=255)
                                    caGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGt                                                              <  1:200672/100‑1 (MQ=255)
                                     aGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGtt                                                             <  1:66986/100‑1 (MQ=255)
                                         aTTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAAc                                                         <  2:613057/100‑1 (MQ=255)
                                         aTTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAAc                                                         <  2:1736210/100‑1 (MQ=255)
                                          ttGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACa                                                        >  1:2497408/1‑100 (MQ=255)
                                             cTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGtt                                                     >  1:1186646/1‑100 (MQ=255)
                                              tGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGttt                                                    >  2:761653/1‑100 (MQ=255)
                                               gAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTc                                                   <  2:943749/100‑1 (MQ=255)
                                               gAAGGCGTGATCAGGTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATACATTATGAGACCCGACGCACACCAGGTTAAACAGTTTc                                                   >  2:1446550/1‑100 (MQ=255)
                                                aaGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCt                                                  <  1:2547355/100‑1 (MQ=255)
                                                  ggCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGc                                                <  1:2207797/100‑1 (MQ=255)
                                                  ggCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGc                                                <  1:646287/100‑1 (MQ=255)
                                                   gCGTAATCAGTTATTTCCACTGGTTCGACAACCAGAAAGAAAATACAAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTg                                                 <  2:733043/98‑1 (MQ=255)
                                                      tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa                                            <  2:1650814/100‑1 (MQ=255)
                                                      tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa                                            >  1:1329806/1‑100 (MQ=255)
                                                       gATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAAc                                           >  1:2386125/1‑100 (MQ=255)
                                                        aTCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAAcc                                          <  2:2534023/100‑1 (MQ=255)
                                                          cAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCtt                                        >  2:2465580/1‑100 (MQ=255)
                                                          cAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCtt                                        >  1:643104/1‑100 (MQ=255)
                                                           aGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTc                                       >  2:965167/1‑100 (MQ=255)
                                                           aGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTc                                       >  2:935316/1‑100 (MQ=255)
                                                            gTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCa                                      >  1:2456131/1‑100 (MQ=255)
                                                            gTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCa                                      >  1:365923/1‑100 (MQ=255)
                                                            gTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGATAAAAAGTTTCTGCTCAACCTTCa                                      >  1:201471/1‑100 (MQ=255)
                                                             ttATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAg                                     <  1:2129114/100‑1 (MQ=255)
                                                               aTTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAgg                                    >  2:2473777/1‑99 (MQ=255)
                                                                tttCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGAt                                  >  2:716446/1‑100 (MQ=255)
                                                                  tCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATAc                                <  2:817592/100‑1 (MQ=255)
                                                                  tCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATAc                                >  1:166590/1‑100 (MQ=255)
                                                                  tCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATAc                                <  1:464727/100‑1 (MQ=255)
                                                                    cACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGa                              >  1:545703/1‑100 (MQ=255)
                                                                     aCTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAt                             >  2:28371/1‑100 (MQ=255)
                                                                       tGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAttt                           >  1:58296/1‑100 (MQ=255)
                                                                       tGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAttt                           >  2:343654/1‑100 (MQ=255)
                                                                        ggTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTg                          >  2:2499621/1‑100 (MQ=255)
                                                                        ggTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTg                          >  2:1379347/1‑100 (MQ=255)
                                                                        ggTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTg                          >  2:268742/1‑100 (MQ=255)
                                                                        ggTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTATGCTCAACCTTCAGGATACGATTTg                          >  2:2485318/1‑100 (MQ=255)
                                                                         gTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACAGCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGt                         >  2:1823215/1‑100 (MQ=255)
                                                                         gTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGt                         >  1:1770341/1‑100 (MQ=255)
                                                                          ttCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTc                        >  1:675672/1‑100 (MQ=255)
                                                                            cGACAACCAGAAAGCACATTCGGAAAAGCGATAGGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTGTGCTCAACCTTCAGGATACGATTTGTcag                      >  2:1567099/1‑100 (MQ=255)
                                                                            cGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcag                      <  1:465978/100‑1 (MQ=255)
                                                                             gACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagc                     <  1:1726782/100‑1 (MQ=255)
                                                                              aCAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagca                    >  2:2602829/1‑100 (MQ=255)
                                                                               cAACCAGAAAGCACATTCTAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagcag                   <  2:636497/100‑1 (MQ=255)
                                                                               cAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagcag                   >  2:30985/1‑100 (MQ=255)
                                                                                 aCCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCt                 >  1:745530/1‑100 (MQ=255)
                                                                                  ccAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTg                >  1:351053/1‑100 (MQ=255)
                                                                                  ccAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTg                >  1:1822058/1‑100 (MQ=255)
                                                                                    aGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGan              <  2:2533304/100‑2 (MQ=255)
                                                                                    aGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGTAGCTGAc              <  2:1688870/100‑1 (MQ=255)
                                                                                    aGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAc              >  1:371845/1‑100 (MQ=255)
                                                                                    aGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAc              >  1:440835/1‑100 (MQ=255)
                                                                                       aaGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGGACACCAGGGTAAACAGTTTCTGCGCAACCTTCAGGATACGATTTGTCAGCAGCTGAc              >  2:1332139/1‑97 (MQ=255)
                                                                                         gCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAccgccg         >  1:1163065/1‑100 (MQ=255)
                                                                                         gCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAccgccg         >  1:1085654/1‑100 (MQ=255)
                                                                                          cacaTTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCCGCTGAccgccg         >  2:2216306/1‑99 (MQ=255)
                                                                                            caTTCTAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTc       <  1:2349264/99‑1 (MQ=255)
                                                                                             agtCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGATAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGa     >  1:1762794/3‑100 (MQ=255)
                                                                                             aTTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGa     <  1:937415/100‑1 (MQ=255)
                                                                                             aTTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGa     >  1:385887/1‑100 (MQ=255)
                                                                                                cGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATgg  >  1:2519499/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
TGTTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGG  >  NC_000913/2553112‑2553307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑