Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,753,986	A→T	16.3%	intergenic (+40/+22)	bamE → / ← ratB	lipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,753,986	0	A	T	16.3%	218.3 / 32.9	85	intergenic (+40/+22)	bamE/ratB	lipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein
Reads supporting (aligned to +/- strand):  ref base A (42/29);  new base T (8/6);  total (50/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCA  >  NC_000913/2753887‑2754084                                                                                                    |                                                                                                    tttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                     <  1:1203138/100‑1 (MQ=255)   tAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATg                                                                                                   >  1:1272705/1‑100 (MQ=255)              gtgtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttt                                                                                        <  1:1058830/100‑1 (MQ=255)               tgtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCtttttt                                                                                       <  2:241749/100‑1 (MQ=255)                gtgtTGACCAATATTGATAACAAACCTTCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCtttttta                                                                                      <  2:62736/100‑2 (MQ=255)                gtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATTAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCtttttta                                                                                      <  2:733913/100‑2 (MQ=255)                gtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTt‑c                                                                                     <  1:1070695/100‑1 (MQ=255)                gtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTt‑c                                                                                     <  2:471425/100‑1 (MQ=255)                gtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTt‑c                                                                                     <  2:2543247/100‑1 (MQ=255)                gtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTt‑c                                                                                     >  1:2111638/1‑100 (MQ=255)                   ttGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttttaacg                                                                                   <  1:529727/100‑5 (MQ=255)                    tGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttttaacgt                                                                                  <  1:2430714/100‑6 (MQ=255)                    tGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTt‑ctgtc                                                                                 <  1:29745/100‑1 (MQ=255)                     gACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTt‑ctgtct                                                                                >  1:1636832/1‑100 (MQ=255)                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑ctgtctgt                                                                              >  1:184638/1‑100 (MQ=255)                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑ctgtctgt                                                                              >  2:461434/1‑100 (MQ=255)                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑ctgtctgt                                                                              >  2:743461/1‑100 (MQ=255)                        cAATATTAATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGtt                                                                             <  1:1562155/100‑1 (MQ=255)                          atatTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGttat                                                                           >  1:1803335/1‑100 (MQ=255)                          atatTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGttat                                                                           >  2:2479119/1‑100 (MQ=255)                           tatTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGttatt                                                                          <  1:1996220/100‑1 (MQ=255)                           tatTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGttatt                                                                          <  1:2499450/100‑1 (MQ=255)                           tatTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACATTTTTT‑CTGTCTGttat                                                                           >  1:430274/1‑99 (MQ=255)                            atTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGttattt                                                                         <  1:1795623/100‑1 (MQ=255)                              tGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGttatttat                                                                       >  2:989634/1‑100 (MQ=255)                               gATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGttatttat                                                                       >  1:295442/1‑99 (MQ=255)                                 tAACAAATCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTGAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTc                                                                     >  2:1369038/1‑99 (MQ=255)                                 tAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCg                                                                    <  2:242425/100‑1 (MQ=255)                                    cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTg                                                                 >  1:172499/1‑100 (MQ=255)                                      aaCCTGCGCTGACTGGTAACTAATAATAAAGTTGCTCTCAAAGACTTTAAAAAAGGTTCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGAt                                                               <  2:2246764/100‑1 (MQ=255)                                         cTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGAtttt                                                            <  1:745991/100‑1 (MQ=255)                                           gcgcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTc                                                          >  2:1629741/1‑100 (MQ=255)                                           gcgcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTc                                                          <  2:1804462/100‑1 (MQ=255)                                           gcgcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTc                                                          <  1:1862309/100‑1 (MQ=255)                                             gcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTg                                                        >  2:927356/1‑100 (MQ=255)                                              cTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGc                                                       <  2:1643575/100‑1 (MQ=255)                                                gAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCt                                                      <  2:232160/99‑1 (MQ=255)                                                  gTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCt                                                         >  2:954266/1‑94 (MQ=255)                                                   tGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGtt                                                  <  2:2305148/100‑1 (MQ=255)                                                   tGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGtt                                                  <  1:514260/100‑1 (MQ=255)                                                   tGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGtt                                                  >  1:400188/1‑100 (MQ=255)                                                    ggTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTg                                                 >  1:38366/1‑100 (MQ=255)                                                     gTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGc                                                <  1:357086/100‑1 (MQ=255)                                                       aaCTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCt                                              >  2:2026185/1‑100 (MQ=255)                                                       aaCTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTCATTCGCTGATTTTTTTTCTCGTTGCCt                                              >  2:2272511/1‑100 (MQ=255)                                                         cTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTg                                             >  2:1256871/1‑99 (MQ=255)                                                         cTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGc                                            >  1:70185/1‑100 (MQ=255)                                                         cTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGc                                            <  2:1180399/100‑1 (MQ=255)                                                         cTAATAATAAAGTTGCTCTCAAAGACGTT‑AAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCg                                           >  2:2243017/1‑100 (MQ=255)                                                           aataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGa                                          <  1:921628/100‑1 (MQ=255)                                                            ataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGaa                                         >  1:615595/1‑100 (MQ=255)                                                             taataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAg                                        >  2:501836/1‑100 (MQ=255)                                                                    gTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                 <  1:919918/100‑1 (MQ=255)                                                                    gTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                 >  1:1446496/1‑100 (MQ=255)                                                                       gCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCg                                >  2:330361/1‑98 (MQ=255)                                                                          ctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCg                           <  1:1239896/100‑1 (MQ=255)                                                                           tcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCgg                          >  2:1373389/1‑100 (MQ=255)                                                                            cAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGc                         >  1:449619/1‑100 (MQ=255)                                                                            cAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGc                         >  1:1086214/1‑100 (MQ=255)                                                                            cAAAGACGTTAAAAAAGGTGCTCAACGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGc                         >  2:2048032/1‑100 (MQ=255)                                                                                 gacagaaaaaaGGTGCTCATTGAGCACCTTTTTTAACGTCTGTTATTTATTCGCTGATTTTTATGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATg                     >  2:2376751/6‑100 (MQ=21)                                                                                  cGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTCTTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAAt                      >  2:1445858/1‑97 (MQ=255)                                                                                  acagaaaaaaGGTGCTCATTGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgag                   >  1:614105/5‑100 (MQ=255)                                                                                   gTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTTTTTCGGATCGGCAATgaga                  >  2:886088/1‑100 (MQ=255)                                                                                     tAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAgg                >  2:235750/1‑100 (MQ=255)                                                                                     gaaaaaaGGTGCTCATTGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAgg                >  1:654314/2‑100 (MQ=255)                                                                                     gaaaaaaGGTGCTCATTGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAgg                >  1:941199/2‑100 (MQ=255)                                                                                     gaaaaaaGGTGCTCATTGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAgg                >  1:1450713/2‑100 (MQ=255)                                                                                      aaaaaaGGTGCTCAATGAGTACCTTTTTT‑CTGTCGGTTTTTTATTCGCGTATTTTTCTGTTCGTTGCCTGCGAAGCTCTTTCGGATTGGCAATGAGAggg               <  2:245298/100‑1 (MQ=255)                                                                                       aaaaaGGTGCTCATTGAGCACCTTTTTTAACGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCACTGAGAggg               >  2:198309/1‑100 (MQ=25)                                                                                        aaaaGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCg             >  2:2490421/1‑100 (MQ=255)                                                                                         aaaGGTGCTCATTGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGa            >  1:128750/1‑100 (MQ=255)                                                                                         aaaGGTGCTCATTGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGa            >  1:1405157/1‑100 (MQ=255)                                                                                          aaGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGAt           >  1:535958/1‑100 (MQ=255)                                                                                            gttGCTCATTGAGCAGCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGTATCGGCAATGAGAGGGCGATa          <  2:1157387/97‑1 (MQ=255)                                                                                            ggTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATaa         >  2:628515/1‑100 (MQ=255)                                                                                             gTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATaaa        >  1:820249/1‑100 (MQ=255)                                                                                              tGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAt       <  1:2244560/100‑1 (MQ=255)                                                                                               gCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtc      <  2:1272699/100‑1 (MQ=255)                                                                                               gCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtc      <  2:1865528/100‑1 (MQ=255)                                                                                                cTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtct     <  1:1254481/100‑1 (MQ=255)                                                                                                cTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtct     >  1:1855442/1‑100 (MQ=255)                                                                                                 tCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtctc    >  1:2304673/1‑100 (MQ=255)                                                                                                 tCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtctc    >  2:444316/1‑100 (MQ=255)                                                                                                 tCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtctc    >  2:40653/1‑100 (MQ=255)                                                                                                  cAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTcc   <  2:2278881/100‑1 (MQ=255)                                                                                                   aaTGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCa  <  2:2540926/100‑1 (MQ=255)                                                                                                   aaTGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCa  >  2:2329086/1‑100 (MQ=255)                                                                                                    aTGAGCACCTTTTTT‑CTGTCTGTTCTTTATTCGCTGATTGTTCTGCTCGTTGCCTTCGAAGCTCTGTTGGATCGGCAATGAGGGTGCGATAAGTCTCCa  >  2:2434062/1‑99 (MQ=255)                                                                                                    |                                                                                                    TTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTT‑CTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCA  >  NC_000913/2753887‑2754084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑