BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,194,516	A→G	100%	K551K (AAA→AAG)	yqiK →	PHB family membrane protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,516	0	A	G	100.0%	390.4 / ‑6.7	112	K551K (AAA→AAG)	yqiK	PHB family membrane protein, function unknown
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (50/60); total (51/61)

Only 100 of 115 total aligned reads displayed.

GCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTG  >  NC_000913/3194420‑3194611

gCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCa                                                                                              <  2:2300680/100‑1 (MQ=255)

gCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCa                                                                                              <  2:1166882/100‑1 (MQ=255)

 cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                             >  2:33567/1‑100 (MQ=255)

  tGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCaga                                                                                            <  1:1474171/100‑1 (MQ=255)

  tGATTGACTCCTTGCTCAATGAAATTGCCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGcn                                                                                              <  2:1563486/98‑2 (MQ=255)

   gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                           <  1:847890/100‑1 (MQ=255)

   gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                           >  1:804688/1‑100 (MQ=255)

    aTTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGt                                                                                          <  1:345042/100‑1 (MQ=255)

     ttGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTa                                                                                         >  1:906972/1‑100 (MQ=255)

       gACTACTTGCTCAATGAAATTGGCGTTTCAGGCGGATCACTGGCGGCATTGACTTCACCCTTAACCTCAACAATTTCCGTCGAAGAAAAGGCAGAGTaa                                                                                        <  2:945660/99‑1 (MQ=255)

        aCTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAc                                                                                      >  1:1208180/1‑100 (MQ=255)

        aCTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAc                                                                                      >  1:2231864/1‑100 (MQ=255)

         caccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAcc                                                                                     <  2:1575387/98‑1 (MQ=255)

           ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                   >  1:2085571/1‑100 (MQ=255)

           ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                   >  1:1432738/1‑100 (MQ=255)

           ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                   >  1:981872/1‑100 (MQ=255)

              tGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCtt                                                                                <  1:95296/100‑1 (MQ=255)

                cTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTcc                                                                              <  1:1002467/100‑1 (MQ=255)

                  cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                            >  2:782211/1‑100 (MQ=255)

                  cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                            <  1:532597/100‑1 (MQ=255)

                  cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                            >  2:575979/1‑100 (MQ=255)

                   aaTGTAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCGCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                           <  2:1680845/100‑1 (MQ=255)

                   aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                           <  1:1266153/100‑1 (MQ=255)

                   aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                           >  1:1965859/1‑100 (MQ=255)

                    aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                          <  1:939227/100‑1 (MQ=255)

                     tGAAATTGGCGTTTCAGGCGGCTCACTGGCAGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaa                                                                         >  1:2029411/1‑100 (MQ=255)

                      gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaat                                                                        <  1:1500697/100‑1 (MQ=255)

                      gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaat                                                                        >  1:2393355/1‑100 (MQ=255)

                          ttGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATc                                                                    >  2:583822/1‑100 (MQ=255)

                             gCGTTTCAGGCGGTTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATccc                                                                  >  2:1246707/1‑99 (MQ=255)

                              cGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTg                                                                >  1:1275914/1‑100 (MQ=255)

                                tttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGc                                                              <  2:1040010/100‑1 (MQ=255)

                                 ttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTAAACAACTCCCGTCGAAGAAGAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGcc                                                             >  2:1419448/1‑100 (MQ=255)

                                  tCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGccc                                                            >  1:2423822/1‑100 (MQ=255)

                                   cAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCa                                                           <  2:894908/100‑1 (MQ=255)

                                    aGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaa                                                          >  2:877136/1‑100 (MQ=255)

                                        ggcTCCCTGGCGGCATTGACTTCACCCTCAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaaaaa                                                       <  1:1356262/99‑1 (MQ=255)

                                         gcTCACTGGCGGCATTTACTTCACTCTTAACCTCAACAGCTCCCGTCGTAGAGAAGGCAGAGTAAACCCTCTTCCAGAAATAATCCCTGGCCCAAAAAGc                                                     <  2:1507685/100‑1 (MQ=255)

                                         gcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGc                                                     >  2:769474/1‑100 (MQ=255)

                                          cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGcc                                                    >  1:885055/1‑100 (MQ=255)

                                          cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGcc                                                    <  1:980583/100‑1 (MQ=255)

                                          cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCCGGctttttgggcc                                                    >  2:1664823/1‑90 (MQ=255)

                                           tCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCg                                                   >  1:1803722/1‑100 (MQ=255)

                                            cACTGGCGGCATTGACTTCACCCTTAACCTCAACAGCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                  <  1:2555907/100‑1 (MQ=255)

                                            cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                  >  1:1794993/1‑100 (MQ=255)

                                            cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                  >  1:293801/1‑100 (MQ=255)

                                             aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                                 <  1:1856824/100‑1 (MQ=255)

                                             aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                                 <  1:351187/100‑1 (MQ=255)

                                              cTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgggg                                                >  1:1232376/1‑100 (MQ=255)

                                                     cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                         >  1:1361692/1‑100 (MQ=255)

                                                     cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                         <  2:1062223/100‑1 (MQ=255)

                                                     cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTGCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                         <  2:1720467/100‑1 (MQ=255)

                                                      aTTGACTTGACCCTTAACCTCAGCAACTACCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAAAACAAAAAAAAAAAAGCCGGGGATTTTTta                                        <  2:617175/100‑1 (MQ=255)

                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                        <  2:2534927/100‑1 (MQ=255)

                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                        >  2:841691/1‑100 (MQ=255)

                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                        >  1:1589054/1‑100 (MQ=255)

                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                        <  2:915487/100‑1 (MQ=255)

                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                        >  1:1521200/1‑100 (MQ=255)

                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                        >  1:557883/1‑100 (MQ=255)

                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                        <  2:2535666/100‑1 (MQ=255)

                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                        <  1:769479/100‑1 (MQ=255)

                                                        tGACTTCACCCTTAACCTCAACACCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtata                                      <  2:2283974/100‑1 (MQ=255)

                                                        tGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtata                                      <  2:1838373/100‑1 (MQ=255)

                                                        tGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCAAAAAAGCCGGGGATTTTTta                                        <  2:1410801/98‑1 (MQ=255)

                                                         gACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTatat                                     >  2:344396/1‑100 (MQ=255)

                                                            ttCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTg                                  <  2:1020740/100‑1 (MQ=255)

                                                            ttCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTg                                  <  2:585870/100‑1 (MQ=255)

                                                             tCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGc                                 <  1:2263599/100‑1 (MQ=255)

                                                               aCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGt                               <  2:2477891/100‑1 (MQ=255)

                                                                cccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGtt                              >  1:1677743/1‑100 (MQ=255)

                                                                    tAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc                          <  1:1575995/100‑1 (MQ=255)

                                                                      aCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTGTATCTGCGTTCCGCTa                        >  2:1522433/1‑100 (MQ=255)

                                                                       ccTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaa                       >  1:1912783/1‑100 (MQ=255)

                                                                       ccTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaa                       >  1:2357441/1‑100 (MQ=255)

                                                                        ntCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaa                      >  2:1955282/2‑100 (MQ=255)

                                                                          caacaaCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCTCTGGCCCAAAAAGCCGGGGCTTTTTTATATCTGCGTTCCGCTaaaaa                    >  2:594494/1‑100 (MQ=37)

                                                                            acaacaCGCGGCGAAGAACATGCGCAGTAAACCCTCATCCAGGACTAAGCCCTGGCCCAAAAAGCCGAGGGTGTAAAATAACTGCGTTCACCTaaaaa                    >  2:1705254/1‑98 (MQ=255)

                                                                             caacGCCCGTCGAAGAAAAGGCAGAGTTAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTg                 <  1:95321/100‑1 (MQ=255)

                                                                              aacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc                <  2:230680/100‑1 (MQ=255)

                                                                              aacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc                <  1:782216/100‑1 (MQ=255)

                                                                               acTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa               >  1:1604042/1‑100 (MQ=255)

                                                                                 tCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc                <  2:2602868/97‑1 (MQ=255)

                                                                                  cccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa             <  1:388993/99‑1 (MQ=255)

                                                                                   ccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTCAAAAGTGCAAATg           <  1:1750206/100‑1 (MQ=255)

                                                                                   ccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg           <  1:575982/100‑1 (MQ=255)

                                                                                   acgacaaagaaAAGGCAGAAAAAAGCTTTCACCAGTAAGAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGAAAAt            <  2:2564522/93‑1 (MQ=0)

                                                                                    cGTCGAACAAAAGGCAGAGTAAACCCTCTTCCAGGAATAAACACAAAAAAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGa          <  2:2444153/100‑1 (MQ=18)

                                                                                     gTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt         >  1:1035131/1‑100 (MQ=255)

                                                                                      tCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc        <  2:1232371/100‑1 (MQ=255)

                                                                                       cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGGGTTCCGCTAAAAAGTGCAAACGATCa       >  1:1329676/1‑100 (MQ=255)

                                                                                       cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa       <  1:1643636/100‑1 (MQ=255)

                                                                                        gaagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg      <  1:389372/100‑1 (MQ=255)

                                                                                        gaagaaAAGGCAGAGTAAACCATCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTAGATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg      >  2:1769123/1‑100 (MQ=255)

                                                                                        gaacaaAAGGCAGAGTAAACCCTCTTCCAGGAATAAAAAAAAAAAAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg      <  2:1497829/100‑1 (MQ=12)

                                                                                         aagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAACTGATCAgg     >  1:2014482/1‑100 (MQ=255)

                                                                                         aagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg     <  2:94502/100‑1 (MQ=255)

                                                                                          agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt    >  2:1764357/1‑100 (MQ=255)

                                                                                          agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGACGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt    >  1:2415933/1‑100 (MQ=255)

                                                                                            aaaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg  <  1:104078/100‑1 (MQ=255)

                                                                                            aaaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg  >  2:1875867/1‑100 (MQ=255)

GCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTG  >  NC_000913/3194420‑3194611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`