Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,298,809 A→G 100% V13A (GTG→GCG)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,298,8090AG86.6% 301.7 / 32.0 119V13A (GTG→GCG) yraRputative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base A (8/8);  new base G (51/52);  total (59/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 123 total aligned reads displayed.

AACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAG  >  NC_000913/3298713‑3298907
                                                                                                |                                                                                                  
aaCACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCccacca                                                                                                 >  1:552546/1‑100 (MQ=255)
 acacCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAg                                                                                                <  1:491161/100‑1 (MQ=255)
 acacCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAg                                                                                                <  2:1845510/100‑1 (MQ=255)
    ccccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGccc                                                                                             <  1:1820838/100‑1 (MQ=255)
     nccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGcccc                                                                                            >  1:2052483/2‑100 (MQ=255)
     cccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGcccc                                                                                            >  2:2241870/1‑100 (MQ=255)
      ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCg                                                                                           <  2:1863241/100‑1 (MQ=255)
      ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCg                                                                                           >  2:174370/1‑100 (MQ=255)
      ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCg                                                                                           <  2:1479773/100‑1 (MQ=255)
      ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCg                                                                                           <  2:890169/100‑1 (MQ=255)
       cGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGt                                                                                          >  2:253872/1‑100 (MQ=255)
         ggCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTg                                                                                        <  2:2193151/100‑1 (MQ=255)
         ggCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGtt                                                                                         >  2:132403/1‑99 (MQ=255)
           cATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTgcg                                                                                      >  2:787168/1‑100 (MQ=255)
            atatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTgcgc                                                                                     >  2:234334/1‑100 (MQ=255)
              atCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCt                                                                                   >  2:390841/1‑100 (MQ=255)
              atCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCAGCAGCAGGTGACCGCCCGCCAGCTCCGTTgcgc                                                                                     >  2:2346993/1‑98 (MQ=255)
               tCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTg                                                                                  <  2:2211652/100‑1 (MQ=255)
                cGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGt                                                                                 >  1:546766/1‑100 (MQ=255)
                 gCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTa                                                                                >  2:2267408/1‑100 (MQ=255)
                 gCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTa                                                                                >  1:1467363/1‑100 (MQ=255)
                 gCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTa                                                                                >  1:235369/1‑100 (MQ=255)
                 gCCCAACGGACGTCGCGCCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTa                                                                                <  2:976262/100‑1 (MQ=255)
                    cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATc                                                                             <  1:1322276/100‑1 (MQ=255)
                    cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGAAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATc                                                                             >  2:1274157/1‑100 (MQ=255)
                    cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTGTCGGTCCGTTAATCAACATCCACAGCAGGTGACCTCCCGCCATCCCCGCTGCGCCTGTAATc                                                                             >  2:1684105/1‑100 (MQ=255)
                     aaCGGACGTCGCGTCGGCGCAGCAATGGCTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAg                                                                           <  2:2296671/100‑1 (MQ=255)
                      aCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAg                                                                           <  1:2175421/100‑1 (MQ=255)
                       cGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGt                                                                          >  2:973483/1‑100 (MQ=255)
                       cGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGATGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGt                                                                          >  2:1355205/1‑100 (MQ=255)
                           cgtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCt                                                                      >  2:1497580/1‑100 (MQ=255)
                           cgtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACACCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTAcc                                                                       <  2:1082449/99‑1 (MQ=255)
                            gtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTg                                                                     >  1:1598295/1‑100 (MQ=255)
                              cgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGAc                                                                   <  2:2495474/100‑1 (MQ=255)
                               gcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGAcn                                                                  <  2:2052472/100‑2 (MQ=255)
                               gcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACt                                                                  >  2:1245684/1‑100 (MQ=255)
                               gcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACt                                                                  <  1:810502/100‑1 (MQ=255)
                                cgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTc                                                                 <  1:870280/100‑1 (MQ=255)
                                   cgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATc                                                              <  1:1990327/100‑1 (MQ=255)
                                    ggCGCATCAATGGCGATAATTTTCTGGTCGTAAATCAACATACGCAGCAGGTGACCGCGCGTCAGCCCCGTTGAGCCTGTAATCAGTACCTGACTCATc                                                              >  2:494562/1‑99 (MQ=255)
                                    ggCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCt                                                             >  1:210041/1‑100 (MQ=255)
                                     gcgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCtt                                                            <  1:2087375/100‑1 (MQ=255)
                                     gcgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGACCCGTTTCGACTGTAATGAGTACCTGCCTCATCtt                                                            >  2:1615606/1‑100 (MQ=255)
                                      cgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                           >  2:1844326/1‑100 (MQ=255)
                                      cgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                           <  1:2111656/100‑1 (MQ=255)
                                         agcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCt                                                        <  1:584320/100‑1 (MQ=255)
                                         agcaATGGAGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGACTGTAATCAGTACCTGACTCATCTTCg                                                          >  2:1547412/1‑98 (MQ=255)
                                          gcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTc                                                       >  2:2139136/1‑100 (MQ=255)
                                            aaTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCt                                                     >  1:40673/1‑100 (MQ=255)
                                             aTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCtt                                                    <  1:57606/100‑1 (MQ=255)
                                             aTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCtt                                                    <  1:2273294/100‑1 (MQ=255)
                                              tGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCttt                                                   <  1:793517/100‑1 (MQ=255)
                                               ggCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTa                                                  <  1:1265861/100‑1 (MQ=255)
                                                gCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTAc                                                 >  1:1926052/1‑100 (MQ=255)
                                                 cGTTAACTTCCGGTTCGTTAATCAACCGCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTAc                                                 <  2:2348685/99‑1 (MQ=255)
                                                  gTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAg                                               <  2:1155319/100‑1 (MQ=255)
                                                    tAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGaa                                             <  1:1839512/100‑1 (MQ=255)
                                                     aaCTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTGGCTCCTTTACAGAAt                                            >  1:2566393/1‑100 (MQ=255)
                                                       cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                          >  1:508337/1‑100 (MQ=255)
                                                       cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                          >  2:731437/1‑100 (MQ=255)
                                                       cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCCGACTCATCTTCGCTCCTTTACAGAATTg                                          <  1:1313283/100‑1 (MQ=255)
                                                        tttCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGt                                         >  2:512044/1‑100 (MQ=255)
                                                         ttCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTc                                        <  2:1892876/100‑1 (MQ=255)
                                                         ttCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTAATCTTCGCTCCTTTACAGAATTGTc                                        <  2:756930/100‑1 (MQ=255)
                                                                cGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTg                                 >  1:2389320/1‑100 (MQ=255)
                                                                 gTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                                >  2:1779216/1‑100 (MQ=255)
                                                                 gTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                                >  2:2284770/1‑100 (MQ=255)
                                                                 gTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                                <  2:2389306/100‑1 (MQ=255)
                                                                   tAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                              <  1:174372/100‑1 (MQ=255)
                                                                   tAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGGAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATCGTCTGCCTGgc                                >  2:1431115/1‑98 (MQ=255)
                                                                    aaTCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCt                             >  1:1950072/1‑100 (MQ=255)
                                                                      tCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTcc                           >  2:306225/1‑100 (MQ=255)
                                                                       cAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTcc                           <  2:1222823/99‑1 (MQ=255)
                                                                        aaCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                         <  1:1043409/100‑1 (MQ=255)
                                                                        aaCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                         <  2:804217/100‑1 (MQ=255)
                                                                         aCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACg                        >  2:2566553/1‑100 (MQ=255)
                                                                          cATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACgg                       <  1:627496/100‑1 (MQ=255)
                                                                           aTCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                      <  1:1497269/100‑1 (MQ=255)
                                                                           aTCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                      <  1:2261093/100‑1 (MQ=255)
                                                                            tCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTCTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCt                     >  2:1608315/1‑100 (MQ=255)
                                                                              cGCAGCAGGTGACTGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCa                   >  1:1748872/1‑100 (MQ=255)
                                                                               gcagcaGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                  >  1:1929586/1‑100 (MQ=255)
                                                                               gcagcaGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                  >  1:1302540/1‑100 (MQ=255)
                                                                               gcagcaGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                  <  2:441678/100‑1 (MQ=255)
                                                                               gcagcaGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTcn                   <  2:2004048/99‑2 (MQ=255)
                                                                               gcagcaGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                  >  2:1549179/1‑100 (MQ=255)
                                                                                 agcagGTGACCGCCCGCCAGGCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCGTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATg                 <  2:689521/99‑1 (MQ=255)
                                                                                 agcagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGc                <  2:2421525/100‑1 (MQ=255)
                                                                                  gcagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCa               <  1:223019/100‑1 (MQ=255)
                                                                                    agGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAcc             >  1:437578/1‑100 (MQ=255)
                                                                                    agGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAcc             <  1:2373527/100‑1 (MQ=255)
                                                                                     ggTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa            >  2:1375907/1‑100 (MQ=255)
                                                                                      gTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGACTTGCGCTCCACGGCTCATGc                >  2:2005257/1‑95 (MQ=255)
                                                                                          ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt       >  1:1357695/1‑100 (MQ=255)
                                                                                          ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACCCATCTTCGCTCCTTTACAGAATTGTCTCCCTTGCGCTCCACGGCGCATGCACCATGCt        >  2:1871751/1‑99 (MQ=255)
                                                                                          ccccccGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt       <  1:919868/97‑1 (MQ=255)
                                                                                          ccGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt       <  1:138427/100‑1 (MQ=255)
                                                                                            gCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTaa     <  2:2280219/100‑1 (MQ=255)
                                                                                             cccGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt    >  2:2300640/1‑100 (MQ=255)
                                                                                             cccGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt    <  1:512048/100‑1 (MQ=255)
                                                                                                |                                                                                                  
AACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAG  >  NC_000913/3298713‑3298907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: