Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,411,299	T→C	100%	V10A (GTA→GCA)	fis →	global DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,299	0	T	C	99.0%	346.9 / ‑4.5	100	V10A (GTA→GCA)	fis	global DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (39/60);  total (40/60)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAC  >  NC_000913/3411202‑3411394                                                                                                  |                                                                                                aaCAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCAc                                                                                               <  1:1314556/100‑1 (MQ=255)   cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAACTTCTGACGCACTg                                                                                             <  1:1769739/100‑1 (MQ=255)   cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                             <  1:2332167/100‑1 (MQ=255)   cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                             >  2:105926/1‑100 (MQ=255)     gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                           <  2:172178/100‑1 (MQ=255)     gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                           >  2:2440774/1‑100 (MQ=255)      cTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAcc                                                                                          >  2:2246737/1‑100 (MQ=255)      cTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAcc                                                                                          <  1:1437830/100‑1 (MQ=255)       tgtaGGCTTTGGAGGTATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACATCGCGTAAATTCTGACGCACTGACCg                                                                                         <  2:2136144/97‑1 (MQ=255)        ggAGGCGTTGGGGGCATACTTCGAAGATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGt                                                                                        <  1:1201312/100‑1 (MQ=255)        ggAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGt                                                                                        >  1:772228/1‑100 (MQ=255)         gAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGtt                                                                                       <  2:1775950/100‑1 (MQ=255)           ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                     <  1:1880842/100‑1 (MQ=255)             cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTa                                                                                   >  1:1559350/1‑100 (MQ=255)              gTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAc                                                                                  <  1:1306982/100‑1 (MQ=255)               ttGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAcc                                                                                 <  1:872868/100‑1 (MQ=255)               ttGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAcc                                                                                 <  2:1900154/100‑1 (MQ=255)                tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                                <  2:357722/100‑1 (MQ=255)                tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                                >  1:1772502/1‑100 (MQ=255)                tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                                <  1:2377289/100‑1 (MQ=255)                   aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGtt                                                                              <  2:1814563/99‑1 (MQ=255)                   aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                             <  1:2201463/100‑1 (MQ=255)                     gCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAc                                                                           <  2:2312035/100‑1 (MQ=255)                     gCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAc                                                                           <  2:2091362/100‑1 (MQ=255)                      cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAct                                                                          >  1:1346166/1‑100 (MQ=255)                      cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAct                                                                          >  1:1549499/1‑100 (MQ=255)                          cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                      >  1:1455692/1‑100 (MQ=255)                          cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                      >  2:1130676/1‑100 (MQ=255)                            tCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgg                                                                    <  1:1497667/100‑1 (MQ=255)                            tCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgg                                                                    >  2:2243473/1‑100 (MQ=255)                             cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                   >  1:577847/1‑100 (MQ=255)                             cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                   >  1:999539/1‑100 (MQ=255)                               aaaaTTTTGCTTAATTATAAATAAAGAGCTGACAGAACTATATTCGAACAACACGTAAATTCTGACGCACCAACCGTTTCTACCCATAACTCTCAGGacc                                                                 >  2:2171501/1‑98 (MQ=255)                                aaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCa                                                                <  1:1741418/100‑1 (MQ=255)                                 aaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAg                                                               <  2:772223/100‑1 (MQ=255)                                 aaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAg                                                               <  2:338900/100‑1 (MQ=255)                                   ttttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGt                                                             <  2:429490/100‑1 (MQ=255)                                      tGCGTAAAGAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGTCCGTTTCTACCGTTAACTCTCAGGATCAGGTAAc                                                          <  2:308594/100‑1 (MQ=255)                                         gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       <  2:1497516/100‑1 (MQ=255)                                         gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       <  2:569914/100‑1 (MQ=255)                                         gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       <  2:568796/100‑1 (MQ=255)                                         gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                       >  1:1976266/1‑100 (MQ=255)                                              cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                  >  1:804322/1‑100 (MQ=255)                                              cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGAGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                  >  2:1600426/1‑100 (MQ=255)                                               aGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAcc                                                 <  1:424730/100‑1 (MQ=255)                                                gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                                <  1:269302/100‑1 (MQ=255)                                                  aaTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCt                                              >  2:1367323/1‑100 (MQ=255)                                                    tAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGGTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTg                                             >  2:435165/1‑99 (MQ=255)                                                      aaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGt                                          >  1:868711/1‑100 (MQ=255)                                                      aaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGCATCAGGTAACCCAAAAACCCCTGCGt                                          <  2:2510477/100‑1 (MQ=255)                                                       agagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                         <  2:1221862/100‑1 (MQ=255)                                                           cTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTc                                     <  1:1314791/100‑1 (MQ=255)                                                            tGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCg                                    <  1:1434731/100‑1 (MQ=255)                                                            tGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCg                                    <  1:2561950/100‑1 (MQ=255)                                                             gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   >  1:1433155/1‑100 (MQ=255)                                                              aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                  <  2:1933824/100‑1 (MQ=255)                                                              aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                  <  2:325065/100‑1 (MQ=255)                                                              aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                  >  2:2185269/1‑100 (MQ=255)                                                              aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                  <  2:980110/100‑1 (MQ=255)                                                              aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGAGCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                   <  2:1104145/99‑1 (MQ=255)                                                               cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                 <  2:577844/100‑1 (MQ=255)                                                               cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                 >  2:230244/1‑100 (MQ=255)                                                               cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                 >  2:1892755/1‑100 (MQ=255)                                                                aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                                <  2:1574349/100‑1 (MQ=255)                                                                aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                                >  1:33936/1‑100 (MQ=255)                                                                 gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                               >  2:1553351/1‑100 (MQ=255)                                                                 gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                               >  1:2198664/1‑100 (MQ=255)                                                                   aCTATGTTCGGACAACGCGTAAATTCTGACGCACTGACCGGTTCTACCGTTAACTCTCAGGATCAGGTAAACAAAAAGCCCCTGCGTGACTCGGTTa                                <  2:1574372/97‑1 (MQ=255)                                                                   aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                             <  1:1984469/100‑1 (MQ=255)                                                                      aTGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAgg                          >  1:2457671/1‑100 (MQ=255)                                                                      aTGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAgg                          <  1:500056/100‑1 (MQ=255)                                                                       tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                         >  2:667985/1‑100 (MQ=255)                                                                       tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                         >  1:709114/1‑100 (MQ=255)                                                                        gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCTTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                         <  2:1549490/99‑1 (MQ=255)                                                                        gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCa                        >  1:110537/1‑100 (MQ=255)                                                                        gTTCGAACAACGCGTAAATTCTGACGCACTAACCGTTTCTACCGTTAACTCTCAGGATAAAAAAAAAAAAAAACCCCTGCGTGACTCGGTTAAACAGGc                         <  2:2352062/99‑1 (MQ=255)                                                                         ttCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCAc                       <  1:1136544/100‑1 (MQ=255)                                                                          tCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACt                      <  1:97932/100‑1 (MQ=255)                                                                            gAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTga                    >  2:136865/1‑100 (MQ=255)                                                                             aacaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGATAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaa                   >  2:1665758/1‑100 (MQ=255)                                                                              acagcGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                  <  1:478160/100‑1 (MQ=255)                                                                              acaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                  >  1:42591/1‑100 (MQ=255)                                                                                aacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCTAAAACCCCTGCGTGCCTCGGCTGAACAGGCAc                       >  2:2513094/1‑93 (MQ=255)                                                                                aacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaagaa                <  1:73547/100‑1 (MQ=255)                                                                                 acGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                  <  2:1916635/97‑1 (MQ=255)                                                                                 acGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAAc               <  2:914067/100‑1 (MQ=255)                                                                                  cgcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACt              >  2:439242/1‑100 (MQ=255)                                                                                  cacgTAAATTCTGACGCACTGACCGTTTCTACCGCTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAAc               <  1:517529/97‑1 (MQ=255)                                                                                    cgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAt            >  1:159053/1‑100 (MQ=255)                                                                                      tAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAttt          >  2:851819/1‑100 (MQ=255)                                                                                      tAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAttt          <  1:1389838/100‑1 (MQ=255)                                                                                        aaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTg        <  2:2133201/100‑1 (MQ=255)                                                                                         aTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGc       <  1:2031244/100‑1 (MQ=255)                                                                                          ttCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCt      >  2:146097/1‑100 (MQ=255)                                                                                           tCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTc     <  1:1597148/100‑1 (MQ=255)                                                                                           tCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTc     >  1:1707999/1‑100 (MQ=255)                                                                                            cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa    <  1:2218445/100‑1 (MQ=255)                                                                                             tGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCaa   <  2:541390/100‑1 (MQ=255)                                                                                              gACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc  <  1:1399696/100‑1 (MQ=255)                                                                                              gACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc  <  1:548939/100‑1 (MQ=255)                                                                                                  |                                                                                                AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAC  >  NC_000913/3411202‑3411394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑