Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,337,024 G→A 100% S3L (TCG→TTG)  adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,337,0240GA99.1% 382.2 / ‑4.5 112S3L (TCG→TTG) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (66/45);  total (67/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 113 total aligned reads displayed.

CAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGG  >  NC_000913/4336927‑4337120
                                                                                                 |                                                                                                
cAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACGCCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCaaa                                                                                                <  1:245897/100‑1 (MQ=255)
   gTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGAc                                                                                             <  2:774365/100‑1 (MQ=255)
   gTTTGCAGGTAACAGAAAAACACCTGACCCCAGAATATTGCCCGACACCAGCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGAc                                                                                             <  2:1261661/100‑1 (MQ=255)
      tGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGGCCACTTTGTGAGCATCAGCATCCAAAGACATa                                                                                          >  2:1825499/1‑100 (MQ=255)
       gCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATaa                                                                                         >  2:529290/1‑100 (MQ=255)
        cAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAAt                                                                                        <  2:268600/100‑1 (MQ=255)
         aGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAAtt                                                                                       >  1:1872101/1‑100 (MQ=255)
          ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAAtt                                                                                       >  2:2160095/1‑99 (MQ=255)
          ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa                                                                                      <  2:1116478/100‑1 (MQ=255)
          ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa                                                                                      >  2:1948981/1‑100 (MQ=255)
          ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTa                                                                                      >  1:239128/1‑100 (MQ=255)
           gTAACAGAAAAACACCTGATCCCATAATATTACCGGACCCTAACAGAGCGACGGGGATTAAGCCCACTACGTGAGCATCAGCAACCAGAGACAAAATTa                                                                                      >  2:2025115/1‑99 (MQ=255)
            tAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTaaa                                                                                    >  1:292758/1‑100 (MQ=255)
             aaCAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAAc                                                                                   <  2:418397/100‑1 (MQ=255)
               cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTc                                                                                 >  2:2485712/1‑100 (MQ=255)
               cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  2:1732889/1‑100 (MQ=255)
               cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  1:70408/1‑100 (MQ=255)
               cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  1:1826590/1‑100 (MQ=255)
               cAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTc                                                                                 >  1:843565/1‑100 (MQ=255)
                aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTcc                                                                                >  1:2504999/1‑100 (MQ=255)
                 gAAAACCACCTGACCCCATAATATTCCCCGACACCATCAGGGCGACGGAGATTAAGCCCACTCAGTGAGCATCAGCATCCAAAGACACAATTAAACTCCt                                                                               >  2:2274916/1‑100 (MQ=255)
                 gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCt                                                                               >  1:1302780/1‑100 (MQ=255)
                  aaaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTg                                                                              <  1:82519/100‑1 (MQ=255)
                      acacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGaa                                                                          <  1:259587/100‑1 (MQ=255)
                       cacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAg                                                                         <  2:2068156/100‑1 (MQ=255)
                       cacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAg                                                                         <  1:607168/100‑1 (MQ=255)
                        acCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAgg                                                                        <  2:2407175/100‑1 (MQ=255)
                         ccTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGc                                                                       >  2:2436180/1‑100 (MQ=255)
                         ccTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGc                                                                       >  2:1421004/1‑100 (MQ=255)
                          cTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCg                                                                      >  2:1298749/1‑100 (MQ=255)
                           tGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGa                                                                     <  1:2106442/100‑1 (MQ=255)
                           tGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGa                                                                     <  2:1242071/100‑1 (MQ=255)
                            gACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTCAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAg                                                                    <  2:855545/100‑1 (MQ=255)
                               cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCtt                                                                 <  2:481058/100‑1 (MQ=255)
                                ccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                >  1:551626/1‑100 (MQ=255)
                                ccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                >  2:759605/1‑100 (MQ=255)
                                ccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                >  2:773157/1‑100 (MQ=255)
                                 cATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCg                                                               >  2:1505123/1‑100 (MQ=255)
                                  ataataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGt                                                              <  2:70435/100‑1 (MQ=255)
                                   taataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                             <  2:1909664/100‑1 (MQ=255)
                                   gcttaatCCCCGTCACCCTGATGGTGTCGGGGAATAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGt                                                              >  1:1290782/7‑99 (MQ=21)
                                   atctatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                             <  2:304662/97‑1 (MQ=255)
                                    cttaatCCCCGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGa                                                            >  1:617541/6‑100 (MQ=25)
                                      aaatCCCCGACACCATCAAGGTAACGGAAATTAAGCCCACTTAGTGAGCATCAGCATCCAAATACATATTTAAACTCCTTCGAAGACGAGCTTAGTGAc                                                           <  2:1175811/96‑1 (MQ=255)
                                       atTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGagat                                                         >  1:304665/1‑97 (MQ=255)
                                        ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAAt                                                        <  1:1181270/100‑1 (MQ=255)
                                        ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAAt                                                        <  1:1878280/100‑1 (MQ=255)
                                        atCCCCGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGCCATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAAt                                                        >  1:239354/2‑100 (MQ=37)
                                         tCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATa                                                       >  1:2500287/1‑100 (MQ=255)
                                          ccccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaa                                                      >  2:380023/1‑100 (MQ=255)
                                           cccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaaa                                                     >  2:2569685/1‑100 (MQ=255)
                                           cccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaaa                                                     >  2:2500207/1‑100 (MQ=255)
                                               acacCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc                                                 <  2:1986544/100‑1 (MQ=255)
                                               acacCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCACCAGCATCCAAAGACATAATTAATCTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc                                                 <  2:760212/100‑1 (MQ=255)
                                                 acCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAAAATAAATTACGt                                               >  2:838048/1‑100 (MQ=255)
                                                  ccATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc                                              >  2:536169/1‑100 (MQ=255)
                                                  ccATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc                                              <  2:2588730/100‑1 (MQ=255)
                                                  ccATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGta                                              <  2:264489/100‑2 (MQ=255)
                                                    aTCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcat                                            >  1:25148/1‑100 (MQ=255)
                                                    aTCAGGGCGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcat                                            >  1:1363110/1‑100 (MQ=255)
                                                      cAGGCTGACGGAGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatat                                          <  2:308290/100‑1 (MQ=255)
                                                           tGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATAc                                     >  1:1940148/1‑100 (MQ=255)
                                                            gACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACg                                    >  2:1676291/1‑100 (MQ=255)
                                                              gatctaTTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc                                  <  1:1405364/95‑1 (MQ=255)
                                                              cGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc                                  <  2:166582/100‑1 (MQ=255)
                                                               ggggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCt                                 >  2:2155273/1‑100 (MQ=255)
                                                                gggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCtc                                <  2:2550174/100‑2 (MQ=255)
                                                                gggATTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTg                                >  1:2063445/1‑100 (MQ=255)
                                                                 ggATTAAGCCCACTTTGTGAGCCTCAGCATCTAAAGATATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGc                               <  2:1756152/100‑1 (MQ=255)
                                                                   aTTAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGccagatc                             >  2:1405357/1‑95 (MQ=255)
                                                                    ttAAGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAtt                            <  1:1591113/100‑1 (MQ=255)
                                                                       aGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                         >  2:325059/1‑100 (MQ=255)
                                                                       aGCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                         >  2:1696204/1‑100 (MQ=255)
                                                                        gCCCACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGAt                        <  2:1748446/100‑1 (MQ=255)
                                                                         cccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa                       >  1:1037083/1‑100 (MQ=255)
                                                                         cccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa                       >  1:1250610/1‑100 (MQ=255)
                                                                         cccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa                       >  2:241394/1‑100 (MQ=255)
                                                                         cccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa                       >  2:238630/1‑100 (MQ=255)
                                                                         cccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa                       <  1:74770/100‑1 (MQ=255)
                                                                          ccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                      <  2:1062382/100‑1 (MQ=255)
                                                                          ccACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                      <  1:759610/100‑1 (MQ=255)
                                                                           cACTTTGTGAGCATCAGCATCCAAAGACATAATTAATCTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                     <  2:1551892/100‑1 (MQ=255)
                                                                           cACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                     >  1:1737378/1‑100 (MQ=255)
                                                                           cACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                     >  1:380067/1‑100 (MQ=255)
                                                                           cACTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                     >  1:1832455/1‑100 (MQ=255)
                                                                             cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg                   <  1:231546/100‑1 (MQ=255)
                                                                             cTTTGTGAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTg                   <  1:2423245/100‑1 (MQ=255)
                                                                                tgtgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat                <  1:2145541/100‑1 (MQ=255)
                                                                                  tgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatta              <  2:1705019/100‑1 (MQ=255)
                                                                                  tgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatta              >  1:25494/1‑100 (MQ=255)
                                                                                  tgAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatta              >  2:1932136/1‑100 (MQ=255)
                                                                                   gAGCATCAGCATCCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattat             >  2:2482474/1‑100 (MQ=255)
                                                                                     cgatctgcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAAttatta           <  1:1445375/94‑1 (MQ=255)
                                                                                      catcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATtattat          >  2:1432697/1‑100 (MQ=255)
                                                                                       atcagcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATc         >  2:1971621/1‑100 (MQ=255)
                                                                                          agcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAc      >  2:147067/1‑100 (MQ=255)
                                                                                           gcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAAttattaagatcg     >  2:1445367/1‑94 (MQ=255)
                                                                                           gcatcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAcc     >  1:489269/1‑100 (MQ=255)
                                                                                            catcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg    <  1:2155285/100‑1 (MQ=255)
                                                                                              tcCAAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCggg  >  2:1695462/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGG  >  NC_000913/4336927‑4337120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑