BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,397,557	G→T	100%	G49V (GGT→GTT)	mutL →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,397,557	0	G	T	100.0%	494.1 / ‑6.7	149	G49V (GGT→GTT)	mutL	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (67/81); minor base C (1/0); total (68/81)

Only 100 of 149 total aligned reads displayed.

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGC  >  NC_000913/4397460‑4397653

ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                                >  2:2526430/1‑100 (MQ=255)

 gtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgg                                                                                               <  1:450981/100‑1 (MQ=255)

  tgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTggg                                                                                              <  1:2362611/100‑1 (MQ=255)

   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                             <  1:748300/100‑1 (MQ=255)

   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                             <  2:1862173/100‑1 (MQ=255)

    atgtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGc                                                                                            >  1:1766605/3‑100 (MQ=255)

    aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGc                                                                                            <  2:421612/100‑1 (MQ=255)

    aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGAAGGTGCGACGCGCATCGATATTGATATCGA‑ACGCGTTgggg                                                                                             >  2:2280911/1‑99 (MQ=255)

     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                           >  2:1800271/1‑100 (MQ=255)

     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                           >  2:1234858/1‑100 (MQ=255)

     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                           >  1:756311/1‑100 (MQ=255)

       tggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGTGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaa                                                                                         >  2:1964024/1‑100 (MQ=255)

        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaaa                                                                                        <  2:1072716/100‑1 (MQ=255)

        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaaa                                                                                        <  2:1221498/100‑1 (MQ=255)

        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaaa                                                                                        >  1:1967685/1‑100 (MQ=255)

        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaaa                                                                                        >  1:2451282/1‑100 (MQ=255)

         gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAAc                                                                                       <  1:2526443/100‑1 (MQ=255)

          tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                      <  1:913731/100‑1 (MQ=255)

           ngAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACtt                                                                                     >  1:259703/2‑100 (MQ=255)

           cGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACtt                                                                                     >  1:1587025/1‑100 (MQ=255)

           cGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACtt                                                                                     >  2:1270625/1‑100 (MQ=255)

           cGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                      <  1:1223382/99‑1 (MQ=255)

            gAGCGACCTGCGGCGGTAGTCAAAGAACTAGTGGAAAACAGCCGCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                      <  2:107158/98‑1 (MQ=255)

                gagcTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                                <  2:2396646/97‑1 (MQ=255)

                gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                                >  1:1054023/1‑100 (MQ=255)

                     gCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑AAGCGTTGGGGCGAAACTTATCCGc                                                                               >  2:228256/1‑96 (MQ=255)

                       gTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGt                                                                         <  2:1446492/100‑1 (MQ=255)

                          ggTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGAt                                                                      <  1:257336/100‑1 (MQ=255)

                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                  >  1:2192391/1‑100 (MQ=255)

                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                  >  1:114954/1‑100 (MQ=255)

                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                  >  2:670790/1‑100 (MQ=255)

                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                  <  2:907617/100‑1 (MQ=255)

                              ctCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAAc                                                                   <  2:1577518/98‑1 (MQ=255)

                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑AAGCGTAGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                                >  1:507755/1‑100 (MQ=255)

                                  aaGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTg                                                              >  2:449444/1‑100 (MQ=255)

                                  aaGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTg                                                              >  2:936315/1‑100 (MQ=255)

                                     aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                           <  2:1358137/100‑1 (MQ=255)

                                      aCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                          <  2:1697438/100‑1 (MQ=255)

                                      aCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                          <  1:366124/100‑1 (MQ=255)

                                      aCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                          <  2:756306/100‑1 (MQ=255)

                                       cTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTa                                                         <  1:1327564/100‑1 (MQ=255)

                                         aGTGTAAACCAGCCTCGATGCAGGTGCGACGCATAGCGATATTGATCTCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTAt                                                        <  2:2129334/99‑1 (MQ=255)

                                         aGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                       >  2:2056135/1‑100 (MQ=255)

                                         aGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                       <  1:2004456/100‑1 (MQ=255)

                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                      >  1:1737668/1‑100 (MQ=255)

                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                      >  1:2172073/1‑100 (MQ=255)

                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                      >  2:152617/1‑100 (MQ=255)

                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                    >  2:1707682/1‑100 (MQ=255)

                                              aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                  <  1:449448/100‑1 (MQ=255)

                                              aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                  >  1:1054824/1‑100 (MQ=255)

                                               aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                 <  1:2375901/100‑1 (MQ=255)

                                               aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                 >  1:1088487/1‑100 (MQ=255)

                                                aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAg                                                <  1:1005799/100‑1 (MQ=255)

                                                 aCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGa                                               >  1:2144750/1‑100 (MQ=255)

                                                  cAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGa                                               <  1:20781/99‑1 (MQ=255)

                                                     ccTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAG                                           >  2:296793‑M1/1‑100 (MQ=255)

                                                      cTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGC                                          >  1:2574259‑M1/1‑100 (MQ=255)

                                                      cTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGC                                          <  1:2202836‑M1/100‑1 (MQ=255)

                                                         gATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAGAAGATGAgctgg                                       <  2:2602091/100‑1 (MQ=255)

                                                         gATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctgg                                       >  2:1784157/1‑100 (MQ=255)

                                                             cAGGTGCGCCGCGTACCGATATTGATAACGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                   <  2:1553273/100‑1 (MQ=255)

                                                             cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                   <  2:450863/100‑1 (MQ=255)

                                                             cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                   >  1:2398238/1‑100 (MQ=255)

                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                 <  2:2093906/100‑1 (MQ=255)

                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                 >  1:2506600/1‑100 (MQ=255)

                                                                gTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                                <  1:2252184/100‑1 (MQ=255)

                                                                 tGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcg                               <  1:1800491/100‑1 (MQ=255)

                                                                    gatctcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgct                             <  1:943615/92‑1 (MQ=37)

                                                                    gACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                            <  1:143701/100‑1 (MQ=255)

                                                                     aCGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTAGCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                            <  2:1848100/99‑1 (MQ=255)

                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGgc                          >  2:423389‑M2/1‑98 (MQ=255)

                                                                      cgcgTAGCGATATTGATATCGA‑ACGCGTTGGGGAGAAACTTATCCGCATTCGTGATAACGGAAAAAAAAAAAAAAAAGATGAgctggcgctggcgctggc                          <  2:1287882/100‑1 (MQ=255)

                                                                       cgcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                          >  1:1326/6‑100 (MQ=37)

                                                                        cgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGgctc                        <  1:343692‑M2/100‑5 (MQ=255)

                                                                         gTATCGATATTGATATCTA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCcagcgccagcgccagctca                       <  2:693976‑M1/100‑20 (MQ=255)

                                                                         gTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCg                       <  1:532065/100‑1 (MQ=255)

                                                                         cgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctagatc                        >  2:943610/4‑95 (MQ=37)

                                                                          tATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCcagcgccagcgccagctcat                      <  2:2584895‑M1/100‑21 (MQ=255)

                                                                          tATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGt                      >  2:1376331/1‑100 (MQ=255)

                                                                           aTCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTc                     <  1:2003101/100‑1 (MQ=255)

                                                                            tCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                    >  1:1656923/1‑100 (MQ=255)

                                                                            tCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGAGGAGCTGGCGCTGGCGCTGGCTCGTCa                    <  1:1582680/100‑1 (MQ=255)

                                                                            tCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGAATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                    <  2:1603664/100‑1 (MQ=255)

                                                                             cGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCcagcgccagcgccagctcatctt                   <  1:296796‑M1/100‑24 (MQ=255)

                                                                             cGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCcagcgccagcgccagctcatctt                   <  1:814684‑M1/100‑24 (MQ=255)

                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCcagcgccagcgccagctcatcttttttg              <  1:1794912‑M1/100‑29 (MQ=255)

                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac              >  1:2275619/1‑100 (MQ=255)

                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac              >  1:822354/1‑100 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc             <  2:1054018/100‑1 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc             <  1:1619450/100‑1 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc             <  1:989124/100‑1 (MQ=255)

                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc             <  1:1844886/100‑1 (MQ=255)

                                                                                     atatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAg           >  1:1129428/1‑100 (MQ=255)

                                                                                       atCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCcagcgccagcgccagctcatcttttttgatacc         >  1:1404864‑M1/1‑67 (MQ=255)

                                                                                       atCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa         <  1:2062101/100‑1 (MQ=255)

                                                                                        tCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaa        <  1:765089/100‑1 (MQ=255)

                                                                                         cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCcagcgccagcgccagctcatcttttttgataccgc       <  2:1404857‑M1/100‑36 (MQ=255)

                                                                                         cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa       >  1:1881982/1‑100 (MQ=255)

                                                                                         cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa       >  1:1945191/1‑100 (MQ=255)

                                                                                          gA‑ACGCGTTGGGGCGAAACTTATCAGCATTGGTGAGAAAGGCTGAGGTATCAAAAAAGATGAGCTGGCGATGGCGGTGGCTCGTTATGGCACGAGTaaaa      >  2:2398243/1‑100 (MQ=255)

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGC  >  NC_000913/4397460‑4397653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`