| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,029,739 | (A)8→7 | coding (231/1191 nt) | rlmI ← | 23S rRNA m(5)C1962 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,029,732 | 0 | A | . | 95.7% | 188.3 / 8.0 | 49 | coding (238/1191 nt) | rlmI | 23S rRNA m(5)C1962 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (31/14); total (32/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGAAAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACC > NC_000913/1029633‑1029824 | gCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCACCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGACGGGa > 2:769833/1‑100 (MQ=255) cAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGaa > 2:727988/1‑100 (MQ=255) ggCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGaaaa > 2:197657/1‑100 (MQ=255) ccATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGaaaaaa > 2:5117/1‑100 (MQ=255) agcAGATGCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGaaaaaaag < 1:1100805/98‑2 (MQ=255) aTCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGaaaaaaag < 2:855032/100‑2 (MQ=255) aTCAAATTCTCCGGCGATTAAACGATAGCTGTTGATGTCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGaaaaaaag < 2:102539/100‑2 (MQ=255) tctgATGCTCCGGCGATTCAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGTTTGTTGTAAACGGCGGGaaaaaaag < 1:1313968/96‑2 (MQ=255) gATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAAt > 2:1724843/1‑100 (MQ=255) tcCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGa > 2:977570/1‑100 (MQ=255) tcCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGa > 2:418397/1‑100 (MQ=255) ggCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAg < 2:1773580/100‑1 (MQ=255) ggCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCAAGCCAGTCACGCCATTTTTGTTCTTGTTGTAAA‑AAAAAAAAAAAAAAGCAATGTCGATAg < 1:1782082/100‑1 (MQ=255) gATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCCTTTTTGTGCTTGTTGAAAACGACGAG‑AAAAAAAGAAAAGTCGATAGACt > 2:1387941/1‑100 (MQ=255) gATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACt < 2:1692990/100‑1 (MQ=255) gATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACt < 2:1150068/100‑1 (MQ=255) aTTAAACTATAGCTCTCGAGTCAAGTTTTCGACGTCAGATAATCAAGCCATTTTTGTGCTTTTTGCAAACAGC‑TGTAAAAAAATCAATATCGAAAGACt < 1:364304/99‑1 (MQ=255) aaaCGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGa > 1:72762/1‑100 (MQ=255) aaCGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGAc < 1:632479/100‑1 (MQ=255) gCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCaa > 2:1392798/1‑100 (MQ=255) gCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCaa < 1:1012595/100‑1 (MQ=255) tGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAAc > 2:1366241/1‑100 (MQ=255) gTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACg < 1:993619/100‑1 (MQ=255) gTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACg < 2:1054614/100‑1 (MQ=255) ccATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGa > 2:1749999/1‑99 (MQ=255) ccATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAAAGTCCAGAc > 2:1301254/1‑100 (MQ=255) aTCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGAcg < 1:667721/99‑1 (MQ=255) tCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGAcgcg > 1:940669/1‑100 (MQ=255) tttttGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGAcgcgcg < 2:1718966/100‑1 (MQ=255) tttGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGcc > 1:696192/1‑100 (MQ=255) gcgcCAGCCAGTCACGCAACTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAACGCAATGTCGATAGACACAGACGGGTCAAACGTCCAGACGCGCGCCCgg > 1:1327224/1‑100 (MQ=255) cgcCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGa > 2:442567/1‑100 (MQ=255) gccagccaGTCACTCAATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGTGTCAAACGTCCACACGCGCGCCCg < 1:705468/97‑1 (MQ=255) ccagccagTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGAtt > 1:1377700/1‑100 (MQ=255) ccagccagTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGAtt > 2:1768568/1‑100 (MQ=255) cagTCACGCCATTTTTGTGCTTGTTGCAATCGGCGGG‑AAAAAAAGCAATGTCGATAGACGCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGa < 2:599025/100‑1 (MQ=255) cagTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGa > 1:1703616/1‑100 (MQ=255) gTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAg > 1:1554784/1‑100 (MQ=255) cACGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCt > 2:856305/1‑100 (MQ=255) ccGCCTTTTTTTTTCTTTTTTCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGGCCAGACGCGCGCCCGGATTTGCGAAGCTg > 1:1091436/2‑100 (MQ=255) aCGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTg > 2:451313/1‑100 (MQ=255) cGCCATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTgg > 2:1589819/1‑100 (MQ=255) cATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGa < 2:696196/100‑1 (MQ=255) cATTTTTGTGCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAAAGTCCAGACGCGCGCCCGGATTTGCGAAGATGGCg > 2:601835/1‑99 (MQ=255) tgtgCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAgc > 1:1354737/1‑100 (MQ=255) tgCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAgcgc > 2:1329466/1‑100 (MQ=255) gCTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGcgcc > 2:1361334/1‑100 (MQ=255) cTTGTTGCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGcgccg > 2:1746750/1‑100 (MQ=255) ttgttgCAAACGGC‑GGTAAAAAAAGCAATGTGTATAGAGTCAGACGGGTCAAACGTCCATAAGCGCGCCCGGATTTGCGAAGCTTGCGAATAAGcgcc < 1:1124088/98‑1 (MQ=255) tgttgCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCcgcg > 2:1399741/1‑100 (MQ=255) tgttgCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGACCGGATTTGCGAAGCTGGCGAATAAGCGCcgcg > 2:91815/1‑100 (MQ=255) tgCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGc < 2:1292185/100‑1 (MQ=255) tgCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGc > 2:1531034/1‑100 (MQ=255) tgCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGc > 2:1532082/1‑100 (MQ=255) gCAAACGGCGGG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCt > 1:126973/1‑100 (MQ=255) aaCGGCGGGAAAAAAAA‑CAATGACAATAAACACAAACGGGACAAACGTCCACACGCGCACACAGATATGCGAAACCAGCGAATAAgccc > 1:692497/1‑87 (MQ=255) cggcggG‑AAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAAcc < 1:554167/99‑1 (MQ=255) | GCAGGCCATCAGATTCTCCGGCGATTAAACGATAGCTGTCGAGGCCATCTTTTTGCGCCAGCCAGTCACGCCATTTTTGTGCTTGTTGCAAACGGCGGGAAAAAAAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACC > NC_000913/1029633‑1029824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |