Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	107,553	(T)9→8	intergenic (+79/‑152)	lpxC → / → secM	UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	107,545	0	T	.	94.4%	294.1 / 11.4	72	intergenic (+71/‑160)	lpxC/secM	UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base . (35/33);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAC  >  NC_000913/107446‑107640                                                                                                    |                                                                                                tCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGt                                                                                                 >  1:1031918/1‑100 (MQ=255) tCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGt                                                                                                 <  1:606636/100‑1 (MQ=255)    aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttt                                                                                              >  1:327630/1‑100 (MQ=255)        gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                          <  1:7316/100‑1 (MQ=255)        gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                          <  2:1017169/100‑1 (MQ=255)         ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                         >  2:864505/1‑99 (MQ=255)         ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                         <  2:1389340/100‑2 (MQ=255)         ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                          <  1:293830/99‑1 (MQ=255)           ttCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaa                                                                                       >  1:1646366/1‑97 (MQ=255)            tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaat                                                                                      >  2:34916/1‑96 (MQ=255)            tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTC‑TTTTTTTTTGAAt                                                                                     <  2:1193547/100‑1 (MQ=255)             cAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaat                                                                                      >  2:1252651/1‑95 (MQ=255)             cAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAtt                                                                                    <  1:1016810/100‑1 (MQ=255)               gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAtttt                                                                                  >  1:840077/1‑100 (MQ=255)                  gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAAtttt                                                                                  <  1:1442650/97‑1 (MQ=255)                  gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTtata                                                                               >  2:163786/1‑100 (MQ=255)                   tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATaa                                                                              >  2:209528/1‑100 (MQ=255)                     cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAaga                                                                            <  2:1563130/100‑1 (MQ=255)                        gCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCt                                                                         <  1:871148/100‑1 (MQ=255)                        gCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAACt                                                                         >  2:1013815/1‑100 (MQ=255)                         cATAACGACATTTATACTGTCGTATAAAATTCTTTTTGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCt                                                                         <  2:913883/99‑1 (MQ=255)                         cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCta                                                                        <  2:699512/100‑1 (MQ=255)                         cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCta                                                                        >  1:289429/1‑100 (MQ=255)                           tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGTTTGTTATAAGAGCtata                                                                      >  1:1236980/1‑100 (MQ=255)                           tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCtata                                                                      <  1:1248742/100‑1 (MQ=255)                             aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATaaa                                                                    >  1:620888/1‑100 (MQ=255)                                 cATTTATACTGTCGTATAAAATTCGATTGGTAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                                <  2:187468/100‑1 (MQ=255)                                 cATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                                <  2:1689455/100‑1 (MQ=255)                                  aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                                >  2:15473/1‑99 (MQ=255)                                  aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACg                                                               <  1:679333/100‑1 (MQ=255)                                  aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACg                                                               <  2:966549/100‑1 (MQ=255)                                    ttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGt                                                             <  2:196650/100‑1 (MQ=255)                                    ttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGt                                                             >  2:640948/1‑100 (MQ=255)                                     tataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTg                                                            <  1:846982/100‑1 (MQ=255)                                      ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                           <  1:1815204/100‑1 (MQ=255)                                       taCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                           <  1:789993/99‑1 (MQ=255)                                         cTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTATTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                        >  1:1488336/1‑100 (MQ=255)                                          tGTCGTATAAAATTCGACTGGTAAATCTGTCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAAc                                                       <  2:91556/100‑1 (MQ=255)                                           tctgTATAAAATTCGACTGGCAAATCTGGCACTCTATCTGTCCAAGTGAACCTGTCG‑GTTGTTCTGAGTTTTATACGAGCAACAAACAACTGTGCg                                                          >  1:1500060/4‑96 (MQ=255)                                           gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                      <  1:834725/100‑1 (MQ=255)                                           gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                      <  1:693409/100‑1 (MQ=255)                                             cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt                                                    >  1:215597/1‑100 (MQ=255)                                              gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                   <  2:840083/100‑1 (MQ=255)                                               tataAATTTCGTCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                   <  2:1031928/99‑1 (MQ=255)                                               tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                  >  2:1712984/1‑100 (MQ=255)                                                ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTT‑TTTTTTTTAAAAGCTATAAAAAACGGTGAGAACGCTGtt                                                 >  1:1509710/1‑100 (MQ=255)                                                ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                 >  2:154266/1‑100 (MQ=255)                                                ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                 >  2:1674149/1‑100 (MQ=255)                                                ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑ATTTTTTTGAATATTATAAAAGCTATAAAAAAAGATGCGAACGcag                                                   >  2:483282/1‑96 (MQ=255)                                                   aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                              >  2:177441/1‑100 (MQ=255)                                                    aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                              <  1:34916/99‑1 (MQ=255)                                                    aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                             >  2:1430163/1‑100 (MQ=255)                                                      ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                             <  1:133432/99‑1 (MQ=255)                                                      ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                           <  1:780833/100‑1 (MQ=255)                                                      ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACCGTGCGAACGCTGTTTTCTTa                                           <  1:1107410/100‑1 (MQ=255)                                                       tCGACGGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTAAAAAAAAAAAAAAAAACGGTGCGAACGCTGTTTTCTTa                                           <  1:1174949/99‑1 (MQ=255)                                                         gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACTAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                        <  2:650382/100‑1 (MQ=255)                                                          aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCa                                       >  1:1467428/1‑100 (MQ=255)                                                             ggCAAATCTGGCACTCTCTCCGACCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATACAAAACCGTGCTAACGCTGTTTTCTTaa                                          >  1:591003/1‑94 (MQ=255)                                                              gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGATTTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACttt                                   >  2:989717/1‑100 (MQ=255)                                                              gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACttt                                   >  2:82697/1‑100 (MQ=255)                                                                 aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                                <  1:640944/100‑1 (MQ=255)                                                                   tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCg                               <  2:327632/99‑1 (MQ=255)                                                                    cTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                             >  1:1469190/1‑100 (MQ=255)                                                                    cTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                             >  1:787734/1‑100 (MQ=255)                                                                     tGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcac                            <  1:1617509/100‑1 (MQ=255)                                                                     tGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcac                            >  2:103300/1‑100 (MQ=255)                                                                     tGGCACTCTATCCGGTCATGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATATAAAACGGTGCGAACGCTGTTTTTTTAAGCACTTTGCCGcac                            <  1:875664/100‑1 (MQ=255)                                                                         aCTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                        >  1:677443/1‑100 (MQ=255)                                                                          ctctctCCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACtt                       >  2:1742770/1‑100 (MQ=255)                                                                           tctctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTa                      >  1:164077/1‑100 (MQ=255)                                                                             tctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATc                    <  1:562224/100‑1 (MQ=255)                                                                               tcCGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCTGCACAACTTATCtt                  >  1:188933/1‑100 (MQ=255)                                                                                 cGGCCAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa                <  2:768591/100‑1 (MQ=255)                                                                                    ccAGGTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTc             >  2:1395339/1‑100 (MQ=255)                                                                                       ggTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGACCGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGt           >  2:283271/1‑99 (MQ=255)                                                                                        gTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGc         <  2:1092221/100‑1 (MQ=255)                                                                                        gTGAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGc         <  1:209527/100‑1 (MQ=255)                                                                                          gAACCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtg       >  2:427377/1‑100 (MQ=255)                                                                                            aCCAGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg     >  2:371485/1‑100 (MQ=255)                                                                                             acAGTCGTTTTTTTTCG‑ATTTTATAAGATTTATAAAAAATGGTGCGAACTCTGTTTGCTTAAGCACTTTTCCGCACATCTTTTCTTCATTCGTGCTGTgg    <  1:1494406/99‑1 (MQ=255)                                                                                               aGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAc  >  2:461643/1‑100 (MQ=255)                                                                                               aGTCG‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAc  >  1:1374282/1‑100 (MQ=255)                                                                                                    |                                                                                                TCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAC  >  NC_000913/107446‑107640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑