| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,370,044 | (T)9→8 | intergenic (+41/‑172) | pspE → / → ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,370,036 | 0 | T | . | 89.7% | 202.7 / 22.7 | 59 | intergenic (+33/‑180) | pspE/ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base . (28/24); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTG > NC_000913/1369937‑1370131 | gggATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGt > 2:1303238/1‑100 (MQ=255) ggATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAAAATTCACCATGAGCGGCGCTTATGCGCCGtt > 2:515838/1‑100 (MQ=255) cccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttt‑tttttc < 2:1326777/100‑2 (MQ=255) ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttt‑tttttct < 1:920444/100‑3 (MQ=255) aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttt‑tttttctgt < 2:940913/100‑5 (MQ=255) cGTTGAGCACTCCGGTGGCATGAAAGACATCGCAATGCCGAAAGTCAAAAGTTAAAAGAATGCACCATGAGCGGCGCTTATGTGCCG‑TT‑TTTTTTCTGTc < 1:444155/100‑1 (MQ=255) cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttt‑tttttct > 2:66798/1‑95 (MQ=255) cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTc > 2:915866/1‑100 (MQ=255) cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTc < 2:480004/100‑1 (MQ=255) gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATTCGTCG‑TT‑TTTTTTCTGTCacac < 1:393343/100‑1 (MQ=255) agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACAcc < 2:279595/100‑1 (MQ=255) agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACAcc > 2:1352790/1‑100 (MQ=255) cGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTAt < 1:1303228/100‑1 (MQ=255) atctggTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTT‑TTTTTTCTGTCACACCTTa < 1:295777/96‑1 (MQ=255) gCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTAtt < 1:128268/100‑1 (MQ=255) cGGTGGCCCGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATTAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTAtttn < 1:132951/100‑2 (MQ=255) gtttgCCTTAAAGATATTGGAATGCCGAAGGTCAATGGTTATAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTAtt < 2:269326/93‑1 (MQ=255) ggTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTT‑TTTTTTCTGTCACACCTTaaga > 2:295779/1‑96 (MQ=255) gTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACa < 2:924608/100‑1 (MQ=255) gCCTGCAAGACATAGCCATGCCGAAGGTCAAAGGTTCAAAGAATTCAACATGCGCGGCGCTTATGCGCCGTTT‑TTTTTTCTGTTAAAAATTAtttttatc > 2:360109/1‑95 (MQ=255) ccTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATcc > 1:240793/1‑99 (MQ=255) cTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATcccc > 1:1614513/1‑100 (MQ=255) aCATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGatt < 1:770482/100‑1 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGcttat > 1:702795/1‑100 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGattat > 2:53251/1‑100 (MQ=255) tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGattatt > 1:1793519/1‑100 (MQ=255) cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGattatt > 2:440991/1‑97 (MQ=255) tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTc > 2:1462245/1‑100 (MQ=255) tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTc < 1:255307/100‑1 (MQ=255) tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCAAACCTTATTTACATCCCCATAGATTATTTGCGTc > 1:63286/1‑100 (MQ=255) gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCa > 2:167495/1‑100 (MQ=255) ccGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAg > 2:929539/1‑100 (MQ=255) cGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTGATTTACATGCCCATAGATTATTTGCGTCAGc > 1:1223029/1‑100 (MQ=255) gAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt < 2:813477/100‑1 (MQ=255) aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCGCACCTTATTTACATCCCCATAGATTATTTGCGTCAGc > 1:1340316/1‑97 (MQ=255) ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcac > 1:457679/1‑100 (MQ=255) ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGATTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTCCCTCCCCATAGATAATTTGCGTCAGCTcac > 1:1710971/1‑100 (MQ=255) gTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcaca < 2:80641/100‑1 (MQ=255) gTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑AT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcaca > 1:634890/1‑100 (MQ=255) cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaaa > 2:609484/1‑100 (MQ=255) cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaaa > 2:1455910/1‑100 (MQ=255) aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa < 2:791886/100‑1 (MQ=255) ggTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACg > 2:762750/1‑100 (MQ=255) ttAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCt < 1:304476/100‑1 (MQ=255) aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCttt < 1:736288/100‑1 (MQ=255) aaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCtttt > 2:184148/1‑100 (MQ=255) aaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCtttt > 2:967602/1‑100 (MQ=255) aaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCttttt < 2:11204/100‑1 (MQ=255) aGAATTCACCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTc < 2:1793532/100‑1 (MQ=255) agaaaaaaaacggcgCATAAGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATCATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg > 1:556410/11‑100 (MQ=255) aCCATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACTCCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTa > 1:927476/1‑100 (MQ=255) ccATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaa > 1:752572/1‑100 (MQ=255) ccATGAGCGGCGCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaa > 2:1506368/1‑100 (MQ=255) cATGAGCGGCGCTTATGCGCCGTTT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaa > 2:1257516/1‑100 (MQ=255) aacggcgCATAAGCGCCGCTT‑TTTTTTTTTTTTTTCCTTATTTACATCCCCATATATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTTGTaaaaa > 1:1775962/3‑99 (MQ=255) aGCGGCGCTTATGCGCCG‑TT‑TTTCTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa < 1:1064925/99‑1 (MQ=255) aGCGGCGCATAAGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTTCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt > 2:1685545/1‑100 (MQ=255) gcggcgCTTATGCGCCG‑TT‑TTTTTTCTGTTACACCTTATTTTCATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt < 2:136957/99‑1 (MQ=255) cggcgCTTATGCGCCGTTTACTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt < 2:800630/100‑1 (MQ=255) cggcgCATAAGCGCCGTTT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt > 2:1638037/1‑99 (MQ=255) ccgcgcTTATGCGCCG‑TT‑TTTTTTCTTTCACGACTTATTTACATCCCCATCGATTATTTGCGTCAGCTCACATATGCGCTTTTTCCCTGGTAAAAAAtt < 1:677891/97‑2 (MQ=255) ggcgCTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAt < 1:1363298/100‑1 (MQ=255) gtgcTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAt < 1:1353654/97‑1 (MQ=255) gcTTATGCGCCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAttt > 2:1261518/1‑99 (MQ=255) atgcgctttt‑ttttttCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc < 2:1804353/93‑1 (MQ=255) cgcCG‑TT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTg < 2:36630/100‑1 (MQ=255) | GGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTT‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTG > NC_000913/1369937‑1370131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |