Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,470,410	(T)7→6	coding (1280/1332 nt)	gtrS →	serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,470,404	0	T	.	100.0%	388.3 / NA	83	coding (1274/1332 nt)	gtrS	serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (40/43);  total (40/43)

ATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCT  >  NC_000913/2470306‑2470498                                                                                                   |                                                                                               aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtt                                                                                               <  2:659561/100‑1 (MQ=255)   tttATGTGTCATTTTTCAGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttt                                                                                             <  2:1404458/100‑1 (MQ=255)      aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttttta                                                                                          >  2:1198181/1‑99 (MQ=255)       tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaa                                                                                         <  2:344924/100‑3 (MQ=255)        gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaat                                                                                        >  2:92984/1‑97 (MQ=255)        gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaat                                                                                        >  1:720526/1‑97 (MQ=255)          gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAAttt                                                                                     >  1:1323289/1‑100 (MQ=255)          gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAAttt                                                                                     <  2:1303739/100‑1 (MQ=255)           tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAATCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTta                                                                                    <  2:684606/100‑1 (MQ=255)            cATTTTTCGGGAAAGGATATAGTGATTTAAGCAAGCATTTATTTTGTATGTATTTTTTGTTCGACCTTTTCTTATACATAACAATCGttttttaagt                                                                                       <  1:1663056/97‑5 (MQ=255)             aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTtata                                                                                  <  2:296230/100‑1 (MQ=255)              tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATaa                                                                                 <  1:1627255/100‑1 (MQ=255)              tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATaa                                                                                 >  1:372941/1‑100 (MQ=255)              tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACATTTGCTTATACATAACAGTCG‑TTTTTTAATTTATaa                                                                                 <  1:1300209/100‑1 (MQ=255)               ttttCGGTGAAGGATTTAGTTATTTAAGCAAACTTTTATTTGGAATGTATTTATCGTTCGACCTATGCTTATACATAACAGTCG‑TTTTTTAATTTATaan                                                                                <  2:541304/100‑2 (MQ=255)                 ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAaat                                                                              >  1:254115/1‑100 (MQ=255)                 ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAaat                                                                              >  1:254114/1‑100 (MQ=255)                    ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAaataat                                                                           >  2:1677231/1‑100 (MQ=255)                      ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTc                                                                         <  2:992379/100‑1 (MQ=255)                         nggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCaaa                                                                      >  1:33480/2‑100 (MQ=255)                          ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAg                                                                     >  2:1346716/1‑100 (MQ=255)                           gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAg                                                                     <  2:720530/99‑1 (MQ=255)                             tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGaaa                                                                  <  1:1076499/100‑1 (MQ=255)                              ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                 <  2:978848/100‑1 (MQ=255)                                 gcgaTTTAAGCAAACATTTATTTGGAATATATTTTTCGTTAGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                              <  1:602011/98‑1 (MQ=255)                                 gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                              <  1:203308/100‑1 (MQ=255)                                 gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                              <  1:181570/100‑1 (MQ=255)                                  agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                             <  2:1575283/100‑1 (MQ=255)                                  agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                             <  2:1806915/100‑1 (MQ=255)                                   gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                            >  1:363380/1‑100 (MQ=255)                                   gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                            >  1:1778621/1‑100 (MQ=255)                                   gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                            >  2:329202/1‑100 (MQ=255)                                      ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACaa                                                         >  1:139066/1‑100 (MQ=255)                                       naagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAt                                                        >  1:1125004/2‑100 (MQ=255)                                         agcaagcaTTTATTTGGAATGTATTTTTCGTTTGACCTTTGCTTATACATAACAGTCG‑TTTCCTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                       >  1:493169/1‑99 (MQ=255)                                           caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                    >  1:1770819/1‑100 (MQ=255)                                           caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                    >  2:662239/1‑100 (MQ=255)                                           caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                    <  2:1525142/100‑1 (MQ=255)                                            aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                   <  2:457746/100‑1 (MQ=255)                                              gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                 <  2:363382/100‑1 (MQ=255)                                                 tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                              <  2:273998/100‑1 (MQ=255)                                                 tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                              <  1:1523935/100‑1 (MQ=255)                                                 tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                              <  1:1560733/100‑1 (MQ=255)                                                  ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATATAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                             >  1:554715/1‑100 (MQ=255)                                                  ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                             >  2:1668275/1‑100 (MQ=255)                                                   tatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                            <  2:1451754/100‑1 (MQ=255)                                                   tatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                            >  1:1709418/1‑100 (MQ=255)                                                   tatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                            >  2:1635069/1‑100 (MQ=255)                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                           <  1:897610/100‑1 (MQ=255)                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                           <  1:99307/100‑1 (MQ=255)                                                     tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                          <  2:778803/100‑1 (MQ=255)                                                     tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                          >  2:381657/1‑100 (MQ=255)                                                       tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                        >  1:1737016/1‑100 (MQ=255)                                                        ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAATAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAGAGCACTa                                       >  1:975503/1‑100 (MQ=255)                                                           aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                    >  1:417912/1‑100 (MQ=255)                                                             gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                  <  2:8171/100‑1 (MQ=255)                                                               ntttttCGTTCGACCGTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                >  2:248607/2‑100 (MQ=255)                                                                tttttCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                               <  2:1740471/100‑1 (MQ=255)                                                                tttttCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                               <  2:956175/100‑1 (MQ=255)                                                                tttttCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                               >  2:160040/1‑100 (MQ=255)                                                                 ttttCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaag                              >  2:405778/1‑99 (MQ=255)                                                                   ttcgttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTg                            <  2:1288079/100‑1 (MQ=255)                                                                     cgttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                          <  2:417914/100‑1 (MQ=255)                                                                      gttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgn                         <  2:1125015/100‑2 (MQ=255)                                                                      gttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                         <  1:213105/100‑1 (MQ=255)                                                                      gttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                         <  1:1206894/100‑1 (MQ=255)                                                                      gttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGAACTAAGTTTAAATTgcgc                         >  2:479283/1‑100 (MQ=255)                                                                        tcgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                       >  2:1607929/1‑100 (MQ=255)                                                                         cgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                      >  1:1401066/1‑100 (MQ=255)                                                                         cgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                      <  2:1717511/100‑1 (MQ=255)                                                                         cgACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                      <  1:412324/100‑1 (MQ=255)                                                                          gACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                     <  2:88582/100‑1 (MQ=255)                                                                              tttGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                 >  1:1483866/1‑100 (MQ=255)                                                                              tttGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                 <  2:1198664/100‑1 (MQ=255)                                                                              nttGCTTATACATAACAGTCG‑TTTTTTATTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                 >  1:197021/2‑100 (MQ=255)                                                                               ttGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                >  2:1476222/1‑100 (MQ=255)                                                                                tGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg               <  2:872643/100‑1 (MQ=255)                                                                                tGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg               >  2:746771/1‑100 (MQ=255)                                                                                 gCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg              <  2:834515/100‑1 (MQ=255)                                                                                    tataCATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc           >  2:692724/1‑100 (MQ=255)                                                                                      taCATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcgc         >  1:1036692/1‑100 (MQ=255)                                                                                      taCATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcgc         <  2:738749/100‑1 (MQ=255)                                                                                       aCATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca        <  1:329200/100‑1 (MQ=255)                                                                                        cATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac       >  2:1419106/1‑100 (MQ=255)                                                                                          tAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa     >  1:348516/1‑100 (MQ=255)                                                                                          tAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa     >  1:1389071/1‑100 (MQ=255)                                                                                           aaCAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg    <  2:861530/100‑1 (MQ=255)                                                                                           aaCAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg    >  1:27158/1‑100 (MQ=255)                                                                                             cAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt  >  2:726415/1‑100 (MQ=255)                                                                                             cAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt  <  2:91455/100‑1 (MQ=255)                                                                                                   |                                                                                               ATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCT  >  NC_000913/2470306‑2470498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑