Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,154,207 (A)9→8 coding (645/660 nt) yghB → general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,154,1990A.91.5% 265.4 / 23.4 72coding (637/660 nt)yghBgeneral envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base . (32/33);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

ACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCG  >  NC_000913/3154108‑3154293
                                                                                           |                                                                                              
aCGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTaaaaaaaat                                                                                        <  1:184094/100‑2 (MQ=255)
   ccATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGTCACGCTGTTTGTGGTGATTaaaaaaaatact                                                                                     >  1:1581779/1‑96 (MQ=255)
    cATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTg                                                                                   <  1:1219844/100‑1 (MQ=255)
     aTGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGt                                                                                  <  2:1140220/100‑1 (MQ=255)
      tGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGTCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGt                                                                                  >  1:993752/1‑99 (MQ=255)
      tGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTa                                                                                 >  1:130654/1‑100 (MQ=255)
       gAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTaaaaaaagatcggaag                                                                                 >  1:1232532/1‑91 (MQ=255)
        aaGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTTATT‑AAAAAAAATACTGTaa                                                                                <  1:273796/99‑1 (MQ=255)
         aGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACg                                                                              <  1:1318891/100‑1 (MQ=255)
          gATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGc                                                                             >  1:412962/1‑100 (MQ=255)
           aTCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACTTTGTTTGTAATGATT‑AAAAAAAATACTGTaa                                                                                <  1:274073/96‑1 (MQ=255)
            tCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAAcccc                                                                            >  2:1100170/1‑100 (MQ=255)
             cAGGTAATGACGTTCCTGATGATCCTGCCAATTACGTTGTTAACCGCTGGCGGGTTAGGGACGCTGTTGGTGGTGA‑TGAAAAAGAATACTGTatc                                                                               >  1:1516645/1‑93 (MQ=255)
               ggTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGAc                                                                        >  2:753695/1‑100 (MQ=255)
               ggTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGAc                                                                        <  1:568370/100‑1 (MQ=255)
               ggTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGAc                                                                        <  1:1388714/100‑1 (MQ=255)
               ggTAATGACGTTCCTGATGATCCCGCCAATTACCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑ACAAAAAATACTGTAACGCCTGAc                                                                        >  1:535950/1‑100 (MQ=255)
                  aaTGACGTTCATGATGATCCTGCCAATTGCCTTGTTAACCACTGGTCTGTTTGGCATGTTTTTTCTGGTGATTAACAAAATATACTGTAACGCCTGACGa                                                                      >  1:1690948/1‑100 (MQ=255)
                    tGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACACCTGACGAttt                                                                   >  2:171826/1‑100 (MQ=255)
                        gTTCCTGATGATCTTGCCAATTGCCTTTTTAACCGCCGGCTTGTTAGGTACGCTGTTTGTGGTGATT‑AAAAAATATACTGTTTCGCCTGACGATTTTcc                                                                <  1:547997/99‑1 (MQ=255)
                        gTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTcc                                                                <  1:85066/100‑1 (MQ=255)
                        gTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTccc                                                               <  1:107444/100‑1 (MQ=255)
                        gTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTccc                                                               <  2:1178297/100‑1 (MQ=255)
                        gTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTccc                                                               <  1:1679587/100‑1 (MQ=255)
                         ttCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑CAAAAAAATACTGTACCGCCTGACGATTTTcccc                                                              <  1:82011/100‑1 (MQ=255)
                         ttCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTcccc                                                              <  2:429322/100‑1 (MQ=255)
                          tCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCg                                                             <  1:1199153/100‑1 (MQ=255)
                             tgatgaTCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCg                                                             >  1:578245/1‑97 (MQ=255)
                               atgatCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTccc                                                        <  1:481794/100‑1 (MQ=255)
                                  atCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCgg                                                      >  2:43974/1‑99 (MQ=255)
                                     cTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTg                                                   >  2:1264127/1‑99 (MQ=255)
                                     cTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGc                                                  >  2:1422275/1‑100 (MQ=255)
                                      tGCCAATTGCGTTGTTAACCACTGGATTGTTAGGCACGCTTTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTcccggttcccggtt                                                    >  1:1297361/1‑97 (MQ=255)
                                         cAATTGCCTTGTTAACCGCTGGCATGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGc                                                  >  1:880053/1‑96 (MQ=255)
                                          aaTTGCCTTGTTAACCGCTGGCTTGTAAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTAc                                             >  2:1103360/1‑100 (MQ=255)
                                           aTTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTAcc                                            <  2:1779062/100‑1 (MQ=255)
                                           aTTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTAc                                             >  1:255607/1‑99 (MQ=255)
                                            ttGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCg                                           >  1:1402229/1‑100 (MQ=255)
                                            ttGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCg                                           >  1:23521/1‑100 (MQ=255)
                                             tGCCTTGTTAAACGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCgg                                          <  2:415138/100‑1 (MQ=255)
                                                cTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGaa                                       >  2:1292162/1‑100 (MQ=255)
                                                 ttGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAAc                                      <  2:110834/100‑1 (MQ=255)
                                                 ttGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTAATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAAc                                      <  1:1512746/100‑1 (MQ=255)
                                                  tGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAAc                                      <  1:864378/100‑1 (MQ=255)
                                                  tGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACg                                     >  1:1576924/1‑100 (MQ=255)
                                                  tGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACg                                     <  2:1577610/100‑1 (MQ=255)
                                                   gTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGt                                    >  2:371896/1‑100 (MQ=255)
                                                     tAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTAt                                  <  2:883173/100‑1 (MQ=255)
                                                       tctgCTGGCTTGTTAAGCACGCTGTTTGTAGTGATT‑AAAAAAAATACTGTCACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTAttt                                <  1:717300/97‑1 (MQ=255)
                                                        ccGCTGGCTTGTTCGGCACGCTGTTTGTTGTTCTT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTAtt                                 <  1:219424/98‑1 (MQ=255)
                                                        ccGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTAttt                                <  1:1130417/100‑1 (MQ=255)
                                                          gCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTaga                             >  2:717304/1‑98 (MQ=255)
                                                          gCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAAt                             <  2:179590/100‑1 (MQ=255)
                                                          gCTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAAt                             <  1:1353287/100‑1 (MQ=255)
                                                           cTGGCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAAtt                            >  2:497273/1‑100 (MQ=255)
                                                             ggCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTcc                          >  2:1679640/1‑100 (MQ=255)
                                                             ggCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGAGAACGTATTTAATTcc                          >  2:37417/1‑100 (MQ=255)
                                                             ggCTTGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCAGGAACGTATTTAATTcc                          >  2:96469/1‑100 (MQ=255)
                                                                ttGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCt                       <  1:1323693/100‑1 (MQ=255)
                                                                 tGTTAGGCACGCTGTTTGTGGTGATT‑ATAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTg                      <  2:1158508/100‑1 (MQ=255)
                                                                 tGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTg                      >  2:1396818/1‑100 (MQ=255)
                                                                 tGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTg                      >  1:555629/1‑100 (MQ=255)
                                                                 tGTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACAGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTg                      >  1:966625/1‑100 (MQ=255)
                                                                  gTTAGGCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCACGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGc                     >  2:579397/1‑100 (MQ=255)
                                                                      ggCACGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATc                  >  2:118293/1‑99 (MQ=255)
                                                                          cGCTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGccc              <  1:451937/99‑1 (MQ=255)
                                                                            cTGTTTGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCTCCATTACCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCa           >  1:115935/1‑100 (MQ=255)
                                                                                ttGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCt       <  2:806156/100‑1 (MQ=255)
                                                                                ttGTGGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCt       <  1:171826/100‑1 (MQ=255)
                                                                                 tgtgGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCt       <  1:1100160/100‑1 (MQ=255)
                                                                                 tgtgGTGATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCtt      <  1:1553676/100‑1 (MQ=255)
                                                                                  gtggtgATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTg     <  1:1745963/100‑1 (MQ=255)
                                                                                    ggtgATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGcc   >  1:1348514/1‑100 (MQ=255)
                                                                                    ggtgATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGcc   >  1:338623/1‑100 (MQ=255)
                                                                                     gtgATT‑AAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCg  <  1:681973/100‑1 (MQ=255)
                                                                                           |                                                                                              
ACGCCATGAAGATCAGGTAATGACGTTCCTGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCG  >  NC_000913/3154108‑3154293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑