| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,512,561 | Δ1 bp | intergenic (‑217/+73) | yhfZ ← / ← trpS | putative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,512,560 | 0 | T | . | 96.0% | 208.8 / 2.3 | 50 | intergenic (‑216/+74) | yhfZ/trpS | putative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (23/25); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
TAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCA > NC_000913/3512462‑3512655 | tAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAta > 2:1168722/1‑99 (MQ=255) aaaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtac < 1:96948/100‑3 (MQ=255) aaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTg < 1:118231/100‑1 (MQ=255) aaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCTTACATTTCCTTGCAATACGTGCA‑TACtttct < 2:1224071/100‑3 (MQ=37) aCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTa < 1:163059/100‑1 (MQ=255) ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAg > 1:684141/1‑100 (MQ=255) ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAg > 2:1204224/1‑100 (MQ=255) ggTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGcc > 2:1429024/1‑100 (MQ=255) tCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGAt > 1:280492/1‑100 (MQ=255) cccGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATa < 1:1239382/99‑1 (MQ=255) cGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAgg < 1:1050150/100‑1 (MQ=255) tttCAGAGAGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGTCCTTGCAATCTGTGCA‑TACTTTGTAGGCCGGATAAGGCGt < 1:1786034/100‑1 (MQ=25) ttCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGtt > 1:1802658/1‑100 (MQ=255) cAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCa > 2:791113/1‑100 (MQ=255) agagTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCAc > 1:1603495/1‑100 (MQ=255) gagTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCAcg < 2:1598764/100‑1 (MQ=255) gatgatAAAAGCAAAATTGCCTGATGCGCTACGCTTATAAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCAcgccgc > 1:28925/1‑100 (MQ=255) gatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1485207/1‑100 (MQ=255) atAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:38911/1‑98 (MQ=255) tAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 2:1399228/1‑100 (MQ=255) cAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa < 2:1730952/100‑1 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc < 2:809188/100‑1 (MQ=255) aTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaa > 2:359007/1‑100 (MQ=255) aTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaa < 1:286441/100‑1 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAg < 2:1624181/100‑1 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAgcgc < 2:933687/100‑1 (MQ=255) gcTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGc > 1:1047425/1‑100 (MQ=255) cTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc < 1:1567092/100‑1 (MQ=255) cTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc > 1:1763185/1‑100 (MQ=255) tACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc < 2:1506939/100‑1 (MQ=255) cGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAg < 2:1199609/100‑1 (MQ=255) gCTTATCAGGCCTACATTTCCTTTCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGc < 1:1368537/99‑1 (MQ=255) gCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCa < 1:166735/100‑1 (MQ=255) cTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCaa < 1:766784/100‑1 (MQ=255) cTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCaa > 1:137732/1‑100 (MQ=255) aTCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGACATGAACAAAGCGCAATTTGCCAGCAATAg > 2:40303/1‑100 (MQ=255) aGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGa > 1:204836/1‑100 (MQ=255) ggCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTg < 1:123425/98‑1 (MQ=255) ggCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGaa > 1:1612504/1‑100 (MQ=255) cTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTa > 2:1262321/1‑100 (MQ=255) tACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTAc > 2:1603856/1‑100 (MQ=255) aCATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAATGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACg < 1:587194/100‑1 (MQ=255) ttCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCtt < 2:1196575/100‑1 (MQ=255) tCCTTGCAATATGTGCA‑TACTTTCTGGGCTGGATAAAGCGTTCCCGCAGCATCAGCTATTAACAAAGCTCAATTTGCCAGTAATAGAGAATTACGGCt < 1:1586834/98‑1 (MQ=255) cTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGc < 2:1802671/100‑1 (MQ=255) ttGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc < 2:137732/100‑1 (MQ=255) ttGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGc < 1:547044/99‑1 (MQ=255) gCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCcac > 2:1531745/1‑100 (MQ=255) cAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCcaca > 1:784417/1‑100 (MQ=255) atatGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACaaa < 2:922749/100‑1 (MQ=255) tgtgCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAcc < 2:1577417/100‑1 (MQ=255) gtgCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCa > 2:1098181/1‑100 (MQ=255) | TAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCA > NC_000913/3512462‑3512655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |