| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,595,315 | (A)5→4 | coding (166/1278 nt) | livM ← | branched‑chain amino acid ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,595,311 | 0 | A | . | 94.9% | 238.4 / 9.9 | 60 | coding (170/1278 nt) | livM | branched‑chain amino acid ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base . (27/29); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
TTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCA > NC_000913/3595212‑3595404 | ttCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGgaaga > 2:1122492/1‑100 (MQ=255) tCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGgaagaa > 1:578602/1‑100 (MQ=255) tCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGgaagaa > 1:578608/1‑100 (MQ=255) tGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaa < 1:1271022/100‑1 (MQ=255) gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaag > 2:727852/1‑99 (MQ=255) gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaag < 1:33603/100‑2 (MQ=255) gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaag < 1:396978/100‑2 (MQ=255) ttCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagac > 2:1473984/1‑97 (MQ=255) ttCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagac < 2:150126/100‑4 (MQ=255) ttCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGa > 2:1094348/1‑100 (MQ=255) ttCACCGTGGAGCCATCAATGGCGGGCAGAATAAAATTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagac > 1:565969/1‑97 (MQ=255) cacccgggAGACAGCAATGGCGGGCACCATAACCTTCTGTCCGTAAATGCTTTTCAACCCTTTCTGCAAAGCCGGTCGCAATAGCTGGAAGaaaaga < 1:755931/91‑3 (MQ=255) cACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGacca > 2:1219831/1‑100 (MQ=255) cGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCAccg > 1:1798915/1‑100 (MQ=255) tGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCAccgcc < 1:741058/100‑1 (MQ=255) cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCg > 1:128993/1‑100 (MQ=255) cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAACTGGAAG‑AAAAGACCACCGCCGTGCCg > 2:585143/1‑100 (MQ=255) aTCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGa > 1:1168413/1‑100 (MQ=255) cAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGAt < 1:1493752/99‑1 (MQ=255) aaTGGCGGGCAGAATAAACTTCTGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGAt < 1:1701822/98‑1 (MQ=255) ggCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAaca > 2:1651727/1‑100 (MQ=255) ggCGGGCAGAATAAACCTCAGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGACGGTCGAAAAAGCTGGAAG‑AAAAGACCACCGACATGACGATAAaca > 1:825010/1‑100 (MQ=255) ggtcataaTAAACATGGGGCGGGTAAGGCGTTCTAACCCTTTCTGGAAACCCGGTAGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATCAac < 1:653534/90‑1 (MQ=255) gggCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACAccc > 1:1551855/1‑100 (MQ=255) cAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACt > 2:166598/1‑100 (MQ=255) aGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAATAGCTGGAAG‑ACAAGACCACCGCCGTGCCGATAAACACCCACt < 2:1224843/99‑1 (MQ=255) aTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTTCCGATAAACACCCACTGCCa < 1:1779387/100‑1 (MQ=255) aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg > 2:1404896/1‑100 (MQ=255) aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg < 2:1551866/100‑1 (MQ=255) aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg < 2:905963/100‑1 (MQ=255) aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAACTGGAAGAAAAA‑ACAACCACAGTGCCAAAAAAAACCCACTGCCAACg > 2:89381/1‑100 (MQ=255) ggTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCTATAAACACCCACTGCCAACGGACATc < 1:40442/100‑1 (MQ=255) gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATcc < 2:1802818/100‑1 (MQ=255) gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATcc < 2:128993/100‑1 (MQ=255) ggAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTTGAAG‑AAAACACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGaa > 2:345598/1‑99 (MQ=255) ggAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAACACCACCGCCGTGCCGATAAACACCCACTGCAAACGGACATCCGaa > 1:151399/1‑100 (MQ=255) gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc < 1:467574/100‑1 (MQ=255) gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc < 1:1255603/100‑1 (MQ=255) gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc < 1:1480895/100‑1 (MQ=255) gAAACGCTCTTTAAGCCTTTCTGTAGAGTCGGCCGCAAAAGCTGGAAG‑AATAGGCCACCGCCGTGCCGATAAACACTCTCTGCCAACGGACATCCGAAGc < 1:19413/100‑1 (MQ=255) aaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCGCTGCCAACGGACATCCGAAGCCg > 1:1411413/1‑100 (MQ=255) aaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCg < 1:1638922/100‑1 (MQ=255) aaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCg < 2:1785332/100‑1 (MQ=255) cGCTTTTCAACCCTTTCTGGAAAGTCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCgt > 1:1238238/1‑99 (MQ=255) cGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCgtg > 1:490562/1‑100 (MQ=255) cGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCgtg < 1:330586/100‑1 (MQ=255) cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc > 2:1588721/1‑100 (MQ=255) cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc < 2:1386591/100‑1 (MQ=255) ttttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCg < 1:1571636/100‑1 (MQ=255) ttttCAACCCTTTCTGGAAAGCCGGTAGCAAAAGCTGGAA‑CAAAAGACCACCGCCGTGCCGATAAACACCAACAGCCAAAGGACAGCCGAAAACGTGTCg > 1:1666075/1‑100 (MQ=255) tttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGa < 2:490565/100‑1 (MQ=255) tttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGa > 2:1695432/1‑100 (MQ=255) ttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGac < 2:104738/100‑1 (MQ=255) cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca > 2:1119777/1‑100 (MQ=255) cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca > 1:1282698/1‑100 (MQ=255) ccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAGCACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAg > 1:1279193/1‑100 (MQ=255) ggAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg > 2:1163930/1‑100 (MQ=255) ggAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg > 1:362831/1‑100 (MQ=255) gAAAGCCGGTCGCAACAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTtgctgc > 1:1428918/1‑100 (MQ=255) gAAAGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg < 2:1300130/99‑1 (MQ=255) aaccGGTCGCAAAGGCTGGAAG‑AAAAGACCAACGCCGTGCCGTTAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCAt > 2:1226195/3‑100 (MQ=255) aGCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCAt < 2:1485711/100‑1 (MQ=255) gCCGGTCGCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATc < 2:701457/100‑1 (MQ=255) gCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCt < 1:1541810/98‑1 (MQ=255) gCAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc < 2:1032487/100‑1 (MQ=255) cAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCa > 2:727581/1‑100 (MQ=255) cAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGAAATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCa > 1:750772/1‑100 (MQ=255) cAAAAGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACAACCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCa > 1:1403582/1‑100 (MQ=255) aaaaGCTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAg < 1:1624744/100‑1 (MQ=255) cTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa < 1:1317692/100‑1 (MQ=255) cTGGAAG‑AAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCCCCAGTTGCa > 1:887118/1‑100 (MQ=255) | TTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCA > NC_000913/3595212‑3595404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |