| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 561,696 | (G)6→7 | coding (2000/2604 nt) | sfmD → | putative outer membrane export usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 561,690 | 1 | . | G | 86.2% | 195.2 / 28.5 | 65 | coding (1994/2604 nt) | sfmD | putative outer membrane export usher protein |
| Reads supporting (aligned to +/- strand): ref base . (5/4); new base G (24/32); total (29/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑G‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGC > NC_000913/561595‑561784 | ccACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑g‑g‑gg > 1:1515329/1‑100 (MQ=255)ccACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑g‑g‑gg > 2:1263291/1‑100 (MQ=255)ccACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑g‑g‑gg > 1:93053/1‑100 (MQ=255)ccACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑g‑g > 1:1346815/1‑98 (MQ=255) aaCCTGAGTTATCGCGTAAGCCAGGGTGATACCTCGAAGAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑g‑g‑gggggc > 1:1121873/1‑98 (MQ=255) aaCCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCAAGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑g‑g‑gggggc > 1:361625/1‑98 (MQ=255) ccTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑G‑G‑GGGGCggg < 1:113869/100‑1 (MQ=255) cTGAGTTATCACGTAAGCCAGGGTGATCCCTCGAATAATGGGTACACGGGCAGCGCCACGGCCCACTGGCAGGCCGCTTACGGTACGC‑t > 1:1020193/1‑89 (MQ=255) cTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑g‑g‑gggggcggg < 2:213123/100‑6 (MQ=255) tGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGt < 1:247476/100‑1 (MQ=255) tGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGt < 2:1510627/100‑1 (MQ=255) gAGTTATCACGTAAGACAGGGTGATACCTCGAACAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑g‑g‑gggggcggg < 2:178814/98‑6 (MQ=255) gTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGtata > 1:1668079/1‑100 (MQ=255) ttATCACGTAAGCCTGGGTTTTACCTCGAATAATGTATACACGGGCAGGGCCTCGGCAAACTGTCAGGCCGCTTTCGGTACGC‑T‑g‑g‑gggggggggtata < 1:413084/99‑6 (MQ=255) ttATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑G‑G‑GGGGCGGGTATAAc < 2:1142356/100‑1 (MQ=255) ttATCACGTAAGCCAGGGTGATACCTCGAATAAGGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATaa < 1:1717696/100‑1 (MQ=255) tATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAAc < 2:1507560/100‑1 (MQ=255) aTCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACt < 1:547907/100‑1 (MQ=255) aTCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACt < 2:505443/100‑1 (MQ=255) aTCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCAACCGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑G‑G‑GGGGCGGGTATAACTa > 1:1095582/1‑100 (MQ=255) cGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGCCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGa > 1:1122152/1‑100 (MQ=255) aaGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACcg < 1:1761523/100‑1 (MQ=255) aGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACcgc > 1:1382076/1‑100 (MQ=255) gCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACcgcg > 2:983874/1‑100 (MQ=255) ccAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACcgc > 1:696388/1‑98 (MQ=255) cAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGAt > 1:1350650/1‑100 (MQ=255) gggTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCa > 2:928121/1‑100 (MQ=255) gggTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCa > 1:1042779/1‑100 (MQ=255) tGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACa < 2:1668090/100‑1 (MQ=255) tGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACa < 2:1658604/100‑1 (MQ=255) aCCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGAc < 2:716709/100‑1 (MQ=255) aCCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGAc < 1:506499/100‑1 (MQ=255) ccTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACg > 1:1210974/1‑100 (MQ=255) ccTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGAATAACTACGACCGCGATCAACATGACg > 2:561176/1‑100 (MQ=255) ctgtaataatGGTTACACGGGCAGCGGCGCGGCAAACTGGCAGGCCGCTTACGGGACGC‑TGG‑G‑GGGGCGGGTATAAGTAGGACCGCGAGCAACATGag < 1:766804/94‑2 (MQ=255) cGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACTC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGtt < 1:1656222/99‑1 (MQ=255) cGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTa > 1:1519969/1‑100 (MQ=255) ataatGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACt < 1:531948/100‑1 (MQ=255) ataatGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑GAG‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACt > 2:1001120/1‑100 (MQ=255) gggTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAg < 2:361627/100‑1 (MQ=255) tACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTg < 2:1306903/100‑1 (MQ=255) cacGGGCAGCGCCACGGCAAACTGGCAGGCCGCTCACGGTACGC‑T‑GAG‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGGCAACTGGCAGCTGTc > 1:1804167/1‑100 (MQ=255) ngggCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTg > 1:1411316/2‑100 (MQ=255) gggCAGCGCCACGGCAAACTGGCAGGACGCTTACGGTACGCAG‑G‑G‑GGGGCGGGTATAACTACGACCGCGATAAACATGACGTTAACTGGCAGCTGTCTgg > 1:1029825/1‑100 (MQ=255) ggCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGc > 1:325130/1‑100 (MQ=255) cgcCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGgtgt < 2:443495/100‑1 (MQ=255) cgcCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGgtgt < 1:983866/100‑1 (MQ=255) ccACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTgg < 2:1121884/100‑1 (MQ=255) ccACGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTgg < 2:1270485/100‑1 (MQ=255) aCGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTc > 1:1511136/1‑100 (MQ=255) cGGCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCg < 1:483625/100‑1 (MQ=255) gCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCggg < 1:1115577/100‑1 (MQ=255) gCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCgg > 1:1414765/1‑99 (MQ=255) gCAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTAGTCgg > 2:389375/1‑99 (MQ=255) cAAACTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGc < 2:250875/100‑1 (MQ=255) aaaCTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCa > 1:348031/1‑100 (MQ=255) aCTGGCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTTTCTGGCGGTGTGGTCGGGCa < 1:844550/98‑1 (MQ=255) ggCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGaaa > 2:212720/1‑100 (MQ=255) ggCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGaaa > 2:428191/1‑100 (MQ=255) ggCAGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGaa < 1:1655771/99‑1 (MQ=255) tgaGGCCGCTTATGGTACGCTT‑G‑T‑TAGAAGGGTATGTCGAGTACCGCGATCAACATGAGGTTAACTGGCAGCTGTCTGGCGTTGTGGTCGGGCATGaaaa < 1:505523/98‑1 (MQ=255) cAGGCCGCTTACAGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAAt > 2:1194285/1‑100 (MQ=255) aGGCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATg > 2:813714/1‑100 (MQ=255) ggCCGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATgg < 1:235027/100‑1 (MQ=255) ccGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCa < 2:1788971/100‑1 (MQ=255) cGCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCAt < 2:486181/100‑1 (MQ=255) gCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATa < 2:118640/100‑1 (MQ=255) gCTTACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATa < 2:865656/100‑1 (MQ=255) gCTTACGGTACGC‑T‑G‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATaa > 2:1149620/1‑100 (MQ=255) tACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGTCAGCTGTCTGGCGGTGTTGTCGGGCATGAAAATGGCATAACg > 1:603249/1‑100 (MQ=255) tACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGTTGTCTGGCGGTGTGGTCGGGCATGGAAATGGCATAACg < 1:1120117/100‑1 (MQ=255) tACGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAAATGGCAGCTGACTAACGGTGTGGTCAAGCATGAAAATGGCATAACg > 2:553696/1‑100 (MQ=255) cGGTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGc < 1:942641/99‑1 (MQ=255) gTACGC‑TGG‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGa > 2:245502/1‑100 (MQ=255) aCGC‑T‑G‑GAGGGGCAGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTTTCCGGCGGTGTGGTCGGTCATGAAAATGGCATAACGCTGagc < 1:1462320/100‑1 (MQ=255) | CCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGC‑T‑G‑G‑GGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTGAGC > NC_000913/561595‑561784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |