Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,143,684 | (G)6→7 | coding (419/3318 nt) | yegE → | putative diguanylate cyclase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,143,678 | 1 | . | G | 92.1% | 206.4 / 13.0 | 64 | coding (413/3318 nt) | yegE | putative diguanylate cyclase |
Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (27/31); total (31/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
CAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTT‑GGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCA > NC_000913/2143579‑2143772 | cAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGtt > 1:1363844/1‑100 (MQ=255) tgctgcTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTT‑ggg < 1:1081080/100‑1 (MQ=255) ctgctACGTAAGTTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTT‑gggg‑g < 1:425426/100‑1 (MQ=255) gctACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTT‑gggg‑ggg > 1:308406/1‑99 (MQ=255) gTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTc < 1:732148/100‑1 (MQ=255) tAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCt < 1:894305/100‑1 (MQ=255) tAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTT‑gggg‑gggag > 2:1655812/1‑93 (MQ=255) ttGCTGCCTTTGTATTATCTCTTTTAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTgg < 2:1628626/98‑1 (MQ=255) ttGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGtt > 2:881755/1‑100 (MQ=255) gctgcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGt < 2:648171/100‑1 (MQ=255) ctgcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTc > 2:269802/1‑100 (MQ=255) tgcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTcc < 1:1353876/100‑1 (MQ=255) tgcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTcc < 2:1358693/100‑1 (MQ=255) gcCGTGGTATAACACAATGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCct < 1:1062095/100‑1 (MQ=255) cGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCctgc < 2:1363854/100‑1 (MQ=255) cGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCctgc > 1:1371699/1‑100 (MQ=255) cGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTT‑GGGG‑GG‑TGTTATGGTTGTCctgct > 2:1736433/1‑100 (MQ=255) gTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCctgct < 1:1611064/100‑1 (MQ=255) gTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCctgct > 1:132478/1‑100 (MQ=255) gTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCCCCTCTGTTAGGGA‑GG‑TGTTCTGGTTGTCctg > 2:757663/1‑98 (MQ=255) tGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCctgctg < 1:481387/100‑1 (MQ=255) tGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCctgctg > 1:1785668/1‑100 (MQ=255) tataACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACg > 2:1153826/1‑100 (MQ=255) tataACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACg > 1:1746640/1‑100 (MQ=255) ccccTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCgg > 2:136739/1‑100 (MQ=255) ccTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCGGACGCCCGgag < 1:1396595/100‑1 (MQ=255) cTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGgaga < 2:1200672/100‑1 (MQ=255) ttGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGAc < 2:385593/100‑1 (MQ=255) tGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGAc < 1:1520740/99‑1 (MQ=255) tGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACg > 2:1516455/1‑100 (MQ=255) tGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACg < 1:1500824/100‑1 (MQ=255) gCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGa > 2:444914/1‑100 (MQ=255) cAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGAt > 1:1149329/1‑100 (MQ=255) cAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGAt > 1:92048/1‑100 (MQ=255) cAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGAt > 2:1112730/1‑100 (MQ=255) aaaTCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATcc < 2:1170292/100‑1 (MQ=255) tGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCa < 1:444912/100‑1 (MQ=255) tGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCa < 2:1214074/100‑1 (MQ=255) cTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAggg < 2:92048/100‑1 (MQ=255) tGACTGGCTGCGTCTGGCACTCGGCAGCGTCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCggg < 2:177003/99‑1 (MQ=255) gACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGATGCCCTGAGAGGATCGTCTCCGGGcg < 1:1563092/100‑2 (MQ=37) gACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCa < 2:1559487/100‑1 (MQ=255) cTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAtt > 1:1334373/1‑100 (MQ=255) gCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAttttt > 2:1178409/1‑100 (MQ=255) gCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAttttt < 2:788484/100‑1 (MQ=255) tGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa > 1:1039034/1‑100 (MQ=255) gCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGATCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCCCAGGGCATTTTTGat > 1:1173709/1‑100 (MQ=255) gTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGatat > 1:6509/1‑100 (MQ=255) tCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGatat < 1:1535326/99‑1 (MQ=255) tCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATg > 1:407556/1‑100 (MQ=255) tGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATggg < 1:837410/100‑1 (MQ=255) ggCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGt > 2:1135974/1‑100 (MQ=255) ggCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGt < 2:1614688/100‑1 (MQ=255) cACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTAc > 2:1169508/1‑100 (MQ=255) cTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACTCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTAc < 1:325337/98‑1 (MQ=255) cGGCAGCTCCATTGTTCCGCCTCTGTT‑GGGG‑GGTTGTTCTGGTTGTCCTGCTGACGCCAGGAGACGTTCCTCTCAGGGCATTTTTGATATGGGTACTGTc < 1:1147025/100‑1 (MQ=255) cGGCAGCGCCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTc < 2:407558/100‑1 (MQ=255) ggCAGCGCCATTGTTCCGCCTCTGTT‑GGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAg > 1:657954/1‑100 (MQ=255) cgcCATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTCATATGGGTACTGTCAGAATc < 1:1112720/100‑1 (MQ=255) ccATTGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCa > 1:782460/1‑100 (MQ=255) tGTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCgg < 2:824454/100‑1 (MQ=255) gTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGTCGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGgc > 2:1278052/1‑100 (MQ=255) gTTCCGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGgc > 2:317327/1‑100 (MQ=255) cGCCTCTGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGctc > 2:15786/1‑100 (MQ=255) ccTCTGTT‑GGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTgg > 1:23919/1‑100 (MQ=255) ctctGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTgg < 2:136625/100‑1 (MQ=255) ctctGTT‑GGGGTGG‑TGTTCTGGTTGTCCTGCTGACGCTCGCAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCGCTgg < 1:277532/100‑1 (MQ=255) ctGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCa > 1:1784477/1‑100 (MQ=255) ctGTTGGGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCa > 1:1455072/1‑100 (MQ=255) | CAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTT‑GGGG‑GG‑TGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCA > NC_000913/2143579‑2143772 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |