| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,809,774 | (T)7→8 | coding (158/738 nt) | ygaZ → | putative L‑valine exporter, norvaline resistance protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,809,767 | 1 | . | T | 94.7% | 245.4 / 6.7 | 75 | coding (151/738 nt) | ygaZ | putative L‑valine exporter, norvaline resistance protein |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base T (31/40); total (33/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGA > NC_000913/2809668‑2809860 | ggATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCg > 1:1304378/1‑100 (MQ=255) cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑tt‑t‑tt > 1:322608/1‑100 (MQ=255) gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAATCG‑ta‑t‑tttttc < 1:949856/100‑3 (MQ=255) aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑tt‑t‑tttttctcc < 1:1426700/100‑6 (MQ=255) tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTg < 1:97080/100‑1 (MQ=255) gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAGTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGcatcat > 1:1037468/1‑100 (MQ=255) gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGcatcat > 2:1137769/1‑100 (MQ=255) ttgttatttgttatATTCCGGTGGCCTTTTCGTTCGTTCTTAATGCGCCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCAttt < 2:1332300/100‑1 (MQ=255) ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCAttt < 2:818618/100‑1 (MQ=255) tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTa < 1:286383/100‑1 (MQ=255) tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTa < 1:286767/100‑1 (MQ=255) gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTAt < 2:346058/100‑1 (MQ=255) ttattaGTTATATTCCGGTGGCTTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATg < 2:75382/100‑1 (MQ=255) tattaGTTATATTCCGGTGGCCTTTTCGTTCGGTCTGAATGCGACCCGTCTGGTATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGc < 1:1434689/100‑1 (MQ=255) tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGc < 2:1304344/100‑1 (MQ=255) tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGc > 2:1106340/1‑100 (MQ=255) attaGTTATATTCCGGTGGCTTTTTCTTTCGGTCTGAGTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCa < 2:1037478/100‑1 (MQ=255) attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCa > 2:1126845/1‑100 (MQ=255) ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATAAATGATGCAggaga < 2:934115/100‑4 (MQ=255) tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGcgcg < 1:390106/100‑1 (MQ=255) atTCCGGTGTCCTTTGCGTTCTGTTTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGc < 2:253669/100‑1 (MQ=255) atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGc < 1:1368445/100‑1 (MQ=255) atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGc < 2:253670/100‑1 (MQ=255) atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATACAGGCGCGAGc > 1:471707/1‑100 (MQ=255) ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGGCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGcc < 1:334437/100‑1 (MQ=255) ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg > 1:716230/1‑100 (MQ=255) cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTTCATCATTTATGCAGTCGCGAGCCAGt > 2:1152222/1‑100 (MQ=255) ggTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGtt > 2:214672/1‑100 (MQ=255) tGGCCTTTGCTTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg < 2:295791/100‑1 (MQ=255) tGGCCTTTGCGTTCGTTCTGAATGCGACTCGTTTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg < 2:752671/100‑1 (MQ=255) ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa < 2:1300279/100‑1 (MQ=255) ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa > 1:240716/1‑100 (MQ=255) cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTTCTTTATCATTTATGCAGGCGCGAGCCAGTTCGTCAt > 1:1604335/1‑100 (MQ=255) tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc < 1:181016/100‑1 (MQ=255) cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg > 1:1309601/1‑100 (MQ=255) ttCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgcg > 2:522903/1‑100 (MQ=255) cGGTCTGAATGCGACCCGTCTGGGATTCTCTCTTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCAGCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGAt < 2:663149/100‑1 (MQ=255) ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg > 1:1748285/1‑100 (MQ=255) tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCATGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg > 1:1110271/1‑100 (MQ=255) tGAATGCGACCCGTCTGGGATACTCTCCTCTCGAAAGCGTTT‑T‑TTTTTTCCTGCATCATTTATGCAGGCGGGAGCCCGTTCGTCATTAc > 1:539717/1‑89 (MQ=255) aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa < 2:1748298/100‑1 (MQ=255) aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa > 1:854670/1‑100 (MQ=255) aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa > 2:1252765/1‑100 (MQ=255) aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa < 1:1402842/100‑1 (MQ=255) aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg > 1:1022543/1‑100 (MQ=255) tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGcc > 1:1221453/1‑100 (MQ=255) aCCCGTCTGGGTTTCTCTCCTCTCGAAGGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg < 2:956767/100‑1 (MQ=255) tCTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATGTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtag < 2:1264189/100‑1 (MQ=255) cTGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCCTTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtagt > 2:250738/1‑100 (MQ=255) tGGGATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt > 1:203379/1‑100 (MQ=255) gggATTCTCTCCTCTCTAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt < 1:1062687/100‑1 (MQ=255) gggATTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt > 1:1115133/1‑100 (MQ=255) ggATTCTCTCCTCTCGAAAGCG‑TT‑TGTTTTCGCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt > 1:810303/1‑98 (MQ=255) ggATTCTCTCCCCTCACAACCGTTT‑T‑TTTTCTCCCGCCTCCTTTATGCAGGCGCTAGCCAGTTCGTCATTACCGCGCTGCTGGCAGCCGGGagtagt < 1:1352117/97‑1 (MQ=255) ggATTCGCTCCTCGCGAAAGCG‑TTGT‑TTTTCTCCTGCATCATTTAGGCAGGCGCGAGCCAGTTCGTCGTTACCGCGATGCTGGCAGCCGGGAGTAGttn < 1:1023566/99‑2 (MQ=255) aTTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg < 1:1342247/100‑1 (MQ=255) aTTCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtg < 2:240717/98‑1 (MQ=255) ttCTCTCCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGTATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg < 2:1475753/100‑1 (MQ=255) ttCTCTCCTCTAGAAAGCGTTT‑T‑TTTTCTCCTGCATAATTTCTGAAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGAAGTAGTTtgtg < 1:733500/99‑1 (MQ=255) tctctcCTCTCGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTTTTCATTACCGCTATGCTGGCAGCCTGGAGTAGTTtgttg < 1:742100/99‑3 (MQ=255) ctctcctctcGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt > 2:108531/1‑100 (MQ=255) tctcctctcGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGACGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt > 1:1008578/1‑100 (MQ=255) ctcctctcGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg < 2:1609038/100‑1 (MQ=255) ctcctctcGAAAGCGTTT‑T‑TTTTCTACTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg > 1:49633/1‑100 (MQ=255) cctctcGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct > 2:778615/1‑100 (MQ=255) cctctcGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgag > 1:1609027/1‑98 (MQ=255) cctctcGAAAGCGTTT‑T‑TTTTCTCCTGCATAATTTATGCAGGCGCGAACCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATttct > 1:154567/1‑100 (MQ=255) ctctcGAAAGCGTTT‑T‑TTTTCTCTTTCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGTAGCCGGGAGTAGTTTGTGGATtgctg < 2:1106980/100‑1 (MQ=255) ctctcGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg < 2:164963/100‑1 (MQ=255) tatcGAGAGCGTTT‑T‑TTTTCTCCTGCAGCATTTGTGCAGGCGCGAGTCAGTTCGTCTTTACCGTGATGCTGGTAGCCTGTAGTAGTTTGTGGATtgctgc < 1:791059/98‑1 (MQ=255) tcGAAAGCGTTT‑T‑TTTTTTCCTGCATCGTTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCGGCCGGGAGTAGTTTGTGGATTGCTGCAc < 2:918094/100‑1 (MQ=255) tcGAAAGCGTTT‑T‑TTTTCTCCTGTATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc < 2:559547/100‑1 (MQ=255) tcGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCTGGTGTGAGCTAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc < 2:491612/100‑1 (MQ=255) tcGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc < 2:934865/100‑1 (MQ=255) tagccaGCG‑TTCT‑TTTTCTCCTGCATCCTTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGAGAGTATTTTGTGTATTGCTGCAc < 1:1516395/95‑1 (MQ=255) cGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCaat > 1:450593/1‑98 (MQ=255) cGAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAca > 1:934859/1‑99 (MQ=255) gAAAGCGTTT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATACTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg > 2:197361/1‑100 (MQ=255) aaGCGTTT‑T‑TTTTCTCCTGCATCATTTTTGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGACAGCCGGGAGTAGTTTGTAGATTGCTGCACTGa > 2:357755/1‑99 (MQ=255) | GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑TT‑T‑TTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGA > NC_000913/2809668‑2809860 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |