| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,854,144 | +G | coding (891/1011 nt) | hypE → | carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,854,140 | 1 | . | G | 87.1% | 216.7 / 28.2 | 71 | coding (887/1011 nt) | hypE | carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein |
| Reads supporting (aligned to +/- strand): ref base . (5/4); new base G (32/29); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
GACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCAC > NC_000913/2854041‑2854235 | gACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACt < 1:1324628/100‑1 (MQ=255)gACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTACTGGCAGCGTTACATTCCCATCCACt > 2:33129/1‑100 (MQ=255) gACGCTTTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑g‑ggg < 2:452285/99‑1 (MQ=255) gACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑g‑gggg < 1:1264696/100‑2 (MQ=255) tccgatctcTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGgaaa < 2:1220135/92‑2 (MQ=255) cccTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGgaaag < 2:1643690/100‑3 (MQ=255) cccTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGgaaag > 1:437509/1‑98 (MQ=255) cccTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGgaaag > 1:437503/1‑98 (MQ=255) cacTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGgaaa < 2:679176/97‑2 (MQ=255) ccTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTAATGGCAGCGTTACATACCCATCCACTGG‑GGGAAAGa > 2:540989/1‑100 (MQ=255) tGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGAcg < 1:1406022/100‑1 (MQ=255) aaacttGCCCACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTCG‑GGGAAAGAcgc < 1:1351424/95‑1 (MQ=255) aCTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACgcgg > 2:1490180/1‑100 (MQ=255) cTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACgcggc > 1:717549/1‑100 (MQ=255) ttGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGgcgc > 2:1205029/1‑100 (MQ=255) tggcgaaCCAAGGCAATCTAGTAAGAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACgcggcg < 1:1434640/94‑1 (MQ=255) tGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCt < 2:1366607/100‑1 (MQ=255) tGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCt > 2:924372/1‑100 (MQ=255) tGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGAAAGCAGCGGCGCTg > 1:330058/1‑100 (MQ=255) gCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTg < 2:1458722/100‑1 (MQ=255) gCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTg > 2:1313768/1‑100 (MQ=255) ccAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGACGCTGa > 2:170944/1‑100 (MQ=255) cAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGAt > 1:104269/1‑100 (MQ=255) aCGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGAAacggcgataa > 2:287967/1‑93 (MQ=255) cGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTgg < 2:1683001/100‑1 (MQ=255) cGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTgg > 1:965484/1‑100 (MQ=255) aaGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTg < 1:1652597/100‑1 (MQ=255) aGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGgaga > 2:780982/1‑100 (MQ=255) aGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGa > 1:993975/1‑100 (MQ=255) aCTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAgtggt < 1:1743005/100‑1 (MQ=255) aCTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAgtggt > 2:1335436/1‑100 (MQ=255) tAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGtggtgg < 2:888184/100‑1 (MQ=255) aaTAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACg < 2:1426846/100‑1 (MQ=255) aaTAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACg < 1:387305/100‑1 (MQ=255) aaTAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑GTGGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACg < 1:1124817/100‑1 (MQ=255) aTAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGt < 2:864672/100‑1 (MQ=255) aTAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGt < 2:717553/100‑1 (MQ=255) aGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTaa > 1:232415/1‑100 (MQ=255) gCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTaaa > 2:1507569/1‑100 (MQ=255) gCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTaaa > 2:911170/1‑100 (MQ=255) tGTTGAATGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAACACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAgg < 1:975250/100‑1 (MQ=255) tGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAgg < 1:1490169/100‑1 (MQ=255) gTTGAACGCAACGCGGCAGAGCCAGTGCTGGGAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGgt < 1:1227492/100‑1 (MQ=255) aacgcaacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTc > 1:1066280/1‑100 (MQ=255) acgcaacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCg < 2:366068/100‑1 (MQ=255) caacgcGGTAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCt < 2:855732/100‑1 (MQ=255) caacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCt > 2:861548/1‑100 (MQ=255) caacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCt < 2:1524028/100‑1 (MQ=255) aacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCtt < 1:1397167/100‑1 (MQ=255) aacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCtt > 1:561624/1‑100 (MQ=255) cgGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCg < 1:1555055/100‑1 (MQ=255) ggCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGt > 1:1773628/1‑100 (MQ=255) gCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCgg < 2:395785/99‑1 (MQ=255) gCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGt > 2:1337599/1‑100 (MQ=255) agagCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCt > 2:495279/1‑100 (MQ=255) agagCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCt > 2:414760/1‑100 (MQ=255) agCAAGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGt > 1:1663711/1‑100 (MQ=255) aGTGCTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATgg < 1:1574951/100‑1 (MQ=255) cTGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTg < 1:1756946/100‑1 (MQ=255) tGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGATGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTg < 2:595331/99‑1 (MQ=255) tGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGa > 1:943639/1‑100 (MQ=255) tGGCAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGa > 2:1458134/1‑100 (MQ=255) cAGCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAAc < 2:1410540/100‑1 (MQ=255) gCGTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGa < 1:1325291/100‑1 (MQ=255) gTTACATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAAc > 1:667160/1‑100 (MQ=255) aCATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCt > 1:948433/1‑100 (MQ=255) cATTCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTc > 1:1725638/1‑100 (MQ=255) tCCCATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGAt < 1:924366/100‑1 (MQ=255) ccATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGAttt < 2:388411/100‑1 (MQ=255) cATCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTa > 1:747768/1‑100 (MQ=255) aTCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTAc > 2:721396/1‑100 (MQ=255) aTCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTAc > 1:525552/1‑100 (MQ=255) tCCACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTAcc > 2:480292/1‑100 (MQ=255) ccACTGG‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTAcc < 1:1445274/99‑1 (MQ=255) ccACT‑G‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACcac > 1:445805/1‑100 (MQ=255) | GACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACT‑G‑GGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCAC > NC_000913/2854041‑2854235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |