New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2700550 =	73 (0.980)	6 (0.100)	5/160	9.7	9.8%	intergenic (‑353/+68)	shoB/acpS	toxic membrane protein/holo‑[acyl‑carrier‑protein] synthase 1
	?	NC_000913	2700601 =	51 (0.850)					intergenic (‑404/+17)	shoB/acpS	toxic membrane protein/holo‑[acyl‑carrier‑protein] synthase 1
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

CTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCA  >  NC_000913/2700451‑2700648                                                                                                    |                                                                                                   cTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGt                                                                                                    >  1:1526437/1‑100 (MQ=255) cTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATAAATAATAGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGt                                                                                                    >  2:112755/1‑100 (MQ=255)   cGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTa                                                                                                  >  1:436433/1‑100 (MQ=255)       tttttATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTaaaaa                                                                                              >  2:1301237/1‑100 (MQ=255)          ttATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTa                                                                                           <  1:482143/100‑1 (MQ=255)           tATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTaa                                                                                          <  1:1559175/100‑1 (MQ=255)              gCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAAc                                                                                       >  1:662196/1‑100 (MQ=255)              gCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAAc                                                                                       >  1:338305/1‑100 (MQ=255)              ccTAAAGATTGCAAGTTGATTGTAAAAGATAAGTACAATGATCCAAAATCGCTGATGGTGAGGGTGCATGCTGCACAACATTAAAGTTAAAAAGTAAAAc                                                                                       >  1:866217/2‑100 (MQ=255)               cTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAAcc                                                                                      >  1:245356/1‑100 (MQ=255)               cTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAAcc                                                                                      >  2:795166/1‑100 (MQ=255)               cTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGATGCACAAAATTAAAGTTAAAAAGTACAAcc                                                                                      >  2:1200897/1‑100 (MQ=255)                  aaGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAAccccc                                                                                   <  1:1609502/100‑1 (MQ=255)                   aGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCg                                                                                  <  2:584091/100‑1 (MQ=255)                   aGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCg                                                                                  <  1:1464543/100‑1 (MQ=255)                   aGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCg                                                                                  <  2:851444/100‑1 (MQ=255)                     aTTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGtt                                                                                <  1:1439574/100‑1 (MQ=255)                      ttGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCTCTGTTGTTGAGAGTTCATACTGCACAAAATTAAAGATAAAAAGTaaaa                                                                                        >  2:571675/1‑91 (MQ=255)                      ttGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTc                                                                               <  2:1657674/100‑1 (MQ=255)                      ttGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCACCGt                                                                                 >  2:1773750/1‑98 (MQ=255)                       tGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTcc                                                                              <  2:256728/100‑1 (MQ=255)                         cAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCtt                                                                            <  1:1141490/100‑1 (MQ=255)                          aaGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTa                                                                           <  2:436435/100‑1 (MQ=255)                           aGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTAc                                                                          >  1:202181/1‑100 (MQ=255)                             ttGCTTGTAAAAGATAAGTACACTTATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAATTTAAAGTTAAAAAGTAAAACCCCCGTTCCtt                                                                            <  1:856971/96‑1 (MQ=255)                              tGCTTGTAATAGATAAGTACACTGGTCCATAATCGCTCTTGTTGAGGTTGCATGCTGCACAAAATTTATGTTATAAAGTAAAAACCCCGTGCCTTACCAg                                                                       <  1:570405/100‑1 (MQ=255)                              tGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAg                                                                       >  2:786708/1‑100 (MQ=255)                              tGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAg                                                                       >  2:1172015/1‑100 (MQ=255)                                cTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGtt                                                                     >  2:1419874/1‑100 (MQ=255)                                  tGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCg                                                                   <  2:1273329/100‑1 (MQ=255)                                   gTAAAAGCTAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGATAAAAAGTAAAACCCCCGTTCCTTACCAGTTCgg                                                                  >  1:955828/1‑100 (MQ=255)                                   gTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCgg                                                                  >  1:826790/1‑100 (MQ=255)                                   gTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCg                                                                   >  2:1203169/1‑99 (MQ=255)                                    tAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCggg                                                                 <  2:50984/100‑1 (MQ=255)                                    tAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATAAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCggg                                                                 >  2:25242/1‑100 (MQ=255)                                      aaaGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCggggg                                                               <  1:635115/100‑1 (MQ=255)                                         gATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGttt                                                            <  2:380305/100‑1 (MQ=255)                                          aTAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGtttt                                                           <  2:1136301/100‑1 (MQ=255)                                              gTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAATAAAACCCCCGTTCCTTACAAGTTCGGGGGTTTTACtt                                                       >  2:558343/1‑100 (MQ=255)                                               taaaatGATCCATAATCGCTGTTGTTGAGGTTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCTGTTCTTTACCAGTTCGTGGGTTTTACttt                                                      <  1:1519665/95‑1 (MQ=255)                                                 cacTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACttttt                                                    >  1:609500/1‑100 (MQ=255)                                                 cacTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACtttt                                                     >  2:1072114/1‑99 (MQ=255)                                                   cTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTaa                                                  <  1:1653759/100‑1 (MQ=255)                                                    tGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTaaa                                                 <  1:358180/100‑1 (MQ=255)                                                     gATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAag                                                >  2:535521/1‑100 (MQ=255)                                                     gATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAag                                                <  2:1481246/100‑1 (MQ=255)                                                        ccATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAgaga                                             >  1:1778877/1‑100 (MQ=255)                                                         cATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGaa                                            >  1:345764/1‑100 (MQ=255)                                                          aTAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAAc                                           <  2:766503/100‑1 (MQ=255)                                                            aaTCGCTGTTGTTGAGGGTGCATGCTGCACCAAATTAACGTTAAAAAATAAAACCCCCGTTACTTACCAGATCAAGGGTTTTACTAATTAAAaacaac                                           >  2:148703/1‑98 (MQ=255)                                                               cGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGtat                                      <  2:1416421/100‑1 (MQ=255)                                                                    ttgttgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTattatt                                  >  2:730866/1‑99 (MQ=255)                                                                     tgttgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGtattat                                   >  2:1511121/1‑97 (MQ=255)                                                                      gttgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTAttttt                               >  2:1332918/1‑100 (MQ=255)                                                                        tgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTaa                             >  1:1065130/1‑100 (MQ=255)                                                                         gAGTGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAAc                            <  1:759192/100‑1 (MQ=255)                                                                               gCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCaa                      <  2:591466/100‑1 (MQ=255)                                                                                cATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCaat                     <  2:86749/100‑1 (MQ=255)                                                                                 aTGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCaata                    >  1:532663/1‑100 (MQ=255)                                                                                   gctgcACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCaataat                  >  1:425300/1‑100 (MQ=255)                                                                                    ctgcACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAAtt                 >  1:1761109/1‑100 (MQ=255)                                                                                     tgcACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTa                >  2:377335/1‑100 (MQ=255)                                                                                      gcACAATATTAAAGTTAAAAAATAAAACACCAGTTCCTTACCAGTACGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTAc               >  1:449911/1‑100 (MQ=255)                                                                                         caAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCg             >  1:212036/1‑99 (MQ=255)                                                                                              ttAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGcac       >  1:762104/1‑100 (MQ=255)                                                                                              ttAAAGTTAAAAAGTAAAACCCACGTTACTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGcac       >  1:367398/1‑100 (MQ=255)                                                                                               tAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGcaca      >  2:107436/1‑100 (MQ=255)                                                                                               tAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGcaca      <  2:1606820/100‑1 (MQ=255)                                                                                                 aaGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAg    <  2:309610/100‑1 (MQ=255)                                                                                                 aaGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAg    <  1:1172004/100‑1 (MQ=255)                                                                                                  aGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGc   <  2:1319327/100‑1 (MQ=255)                                                                                                   gTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCa  >  1:1363517/1‑100 (MQ=255)                                                                                                   gTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCa  >  2:870300/1‑100 (MQ=255)                                                                                                    |                                                                                                   CTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCA  >  NC_000913/2700451‑2700648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.