| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 60,316 | C→T | E11K (GAA→AAA) | rluA ← | dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 60,316 | 0 | C | T | 98.5% | 225.0 / ‑4.6 | 68 | E11K (GAA→AAA) | rluA | dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (33/34); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
TGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCC > NC_000913/60220‑60409 | tgtgCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGTATAACCAACCAGGGTTTCTg < 1:304274/100‑1 (MQ=255) gtgCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGc > 1:1157378/1‑100 (MQ=255) gtgCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGc < 2:459032/100‑1 (MQ=255) tgCTCTTCCAGACTACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCg < 1:1682005/100‑1 (MQ=255) gCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCgg > 2:199586/1‑100 (MQ=255) tctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTg < 1:548848/100‑1 (MQ=255) tctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTg > 2:1089106/1‑100 (MQ=255) ctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTgg < 2:1103787/100‑1 (MQ=255) ctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGAGGTgg < 1:779572/100‑1 (MQ=255) tCCAGACGACCCGGCACTGACAACAAGCCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGAt > 2:1108432/1‑100 (MQ=255) ccAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCAGTGGAtt > 2:1587395/1‑100 (MQ=255) cAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTg < 2:316431/100‑1 (MQ=255) aGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGt < 2:107740/100‑1 (MQ=255) acgacCCGGTACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAg < 2:1432152/100‑1 (MQ=255) cccGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGtt < 1:1698815/98‑1 (MQ=255) ggCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCa < 1:469837/100‑1 (MQ=255) aCTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATcc < 1:591317/100‑1 (MQ=255) aCTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATcc < 1:972910/100‑1 (MQ=255) tGACAACAAACCGCTCGGCTTGATGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATcctc > 1:1056983/1‑98 (MQ=255) gACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCa > 1:1360352/1‑100 (MQ=255) gACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCa > 2:86618/1‑100 (MQ=255) acaacaAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCAt > 1:1500111/1‑100 (MQ=255) caacaaACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCAtt > 2:1150561/1‑100 (MQ=255) caacaaACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTACGGTGGATTGTAGTTTTCCATCCCCAtt > 2:1014897/1‑100 (MQ=255) aaaCCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCg > 1:202460/1‑100 (MQ=255) aaaCCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCg > 1:670603/1‑100 (MQ=255) cGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCt < 1:548077/99‑1 (MQ=255) cGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTc > 2:200409/1‑100 (MQ=255) cGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTc > 1:637468/1‑100 (MQ=255) cGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTc < 1:881265/100‑1 (MQ=255) gCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCAATCCCCATTTTCGGCTcc > 2:429674/1‑100 (MQ=255) cTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGc < 1:892135/96‑1 (MQ=255) cTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCg > 2:950980/1‑100 (MQ=255) tCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGt > 2:1802883/1‑100 (MQ=255) cGGCTTGTAGACCACCATAATATGGTCATCCTGATAAAGGATAACCAAACAGAGGTTCGACGACGGGTTGTAGCATTCCATCCCCAATCTCGGCTACggt > 1:1401161/1‑98 (MQ=255) gCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTAc < 2:1185265/100‑1 (MQ=255) ttgttgACCACCATAATATGTTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTg < 2:1693470/100‑1 (MQ=255) ttgttgACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTg < 2:405351/100‑1 (MQ=255) gttgACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGAt < 2:476658/100‑1 (MQ=255) gttgACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCAACTCAGTTACTGa > 2:278695/1‑99 (MQ=255) ngACCACCATAATATGGTCATCCAGATACAGGATAACCAACCAGGGTTTCTGCGGTGGCTAGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGAt > 1:393462/2‑98 (MQ=255) gACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCg < 2:480110/100‑1 (MQ=255) ccaccaTAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGtt > 1:240077/1‑100 (MQ=255) accaTAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGTGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTAc > 1:504810/1‑100 (MQ=255) accaTAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTAc > 1:610418/1‑100 (MQ=255) ataataTGGTCATCCTGTTACAGGATAACCAACCAGGGTTCCTGCGGTGGAGTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACaa < 2:1148593/99‑1 (MQ=255) taataTGTTCATCCTGATACATGATAACCAACCAGTTTGTCTTCGGTTGATTGTATTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAAc < 2:1058397/99‑1 (MQ=255) tatGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATc < 1:950973/100‑1 (MQ=255) atGGTTATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTAGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGaac < 1:312025/99‑3 (MQ=255) atGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATc < 2:702284/99‑1 (MQ=255) tGGTCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCaa > 2:1296836/1‑100 (MQ=255) tCATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACg < 1:133841/100‑1 (MQ=255) cATCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGc > 2:867652/1‑100 (MQ=255) tCCTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAg < 1:421872/100‑1 (MQ=255) cTGATACAGTATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAggg < 2:528593/100‑1 (MQ=255) cTGATACAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAggg > 2:1351414/1‑100 (MQ=255) gATACAGGATAACCAACCTGGGTTTCTCCCGTCAATTGTAATTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAggg < 1:1142651/98‑1 (MQ=255) cAGGATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCa < 1:1218895/100‑1 (MQ=255) ggATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGa > 1:846481/1‑100 (MQ=255) ggATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGa > 2:1506207/1‑100 (MQ=255) gATAACCAACCAGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGAc > 2:1050948/1‑100 (MQ=255) ccaaccaGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCaa > 1:340604/1‑100 (MQ=255) ccaaccaGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGAGCATACAGACGCCaa > 2:1665173/1‑100 (MQ=255) caaccaGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAc < 1:1782148/100‑1 (MQ=255) caaccaGCTTTTCTGCGGTGGATTCTAGTGTTCCATCCCCATTTGCGGCTCCGTTACTGATGCTTTACAACGATCAAACGCAGGGCATCCAGACGCCa < 1:1658501/98‑1 (MQ=255) accaGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAcct < 2:1413976/100‑1 (MQ=255) ccaGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAcctg > 2:420542/1‑100 (MQ=255) aGGGTTTCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAcctgcc < 1:1405766/100‑1 (MQ=255) | TGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCC > NC_000913/60220‑60409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |