| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,110,872 | A→G | T4A (ACA→GCA) | opgH → | OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,110,872 | 0 | A | G | 98.5% | 233.1 / ‑4.4 | 68 | T4A (ACA→GCA) | opgH | OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (37/30); total (38/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
GATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGACAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCC > NC_000913/1110775‑1110967 | gATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCa < 1:225433/100‑1 (MQ=255) aTGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCaa > 2:1383023/1‑100 (MQ=255) tGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAAc > 2:1623388/1‑100 (MQ=255) tGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAAc < 2:380670/100‑1 (MQ=255) gCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACt < 2:1200317/100‑1 (MQ=255) aaaaCCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTAc < 1:1645673/100‑1 (MQ=255) aaaaCCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGCGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTAc < 2:1350373/100‑1 (MQ=255) aaaCCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTTAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACa < 1:169006/100‑1 (MQ=255) aaaCCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACa < 1:468811/100‑1 (MQ=255) cTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACg > 2:517159/1‑100 (MQ=255) tGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGc < 2:426648/100‑1 (MQ=255) gCGTGCTGCGCTGGTGAATACCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGTAACTGAGTACATTGACGCAATGcc < 1:870785/100‑1 (MQ=255) tgcGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATcgc < 1:1039610/100‑1 (MQ=255) tgcGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATcgc < 2:1586991/100‑1 (MQ=255) tgcGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATcgc < 2:1019372/100‑1 (MQ=255) tgcGCTGGTGAATGCCGAACAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATcgc > 1:353711/1‑100 (MQ=255) gcgcTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATcgcc > 1:1539784/1‑100 (MQ=255) gcgcTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATcgcc > 2:105170/1‑100 (MQ=255) gcgcTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATcgcc > 2:608509/1‑100 (MQ=255) cgcTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATcgcc > 1:182374/1‑99 (MQ=255) ggTGAATGCCGATCAGACGTTGAGTGAAAGCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAg < 1:1088161/100‑1 (MQ=255) gAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCga > 2:1094186/1‑100 (MQ=255) aaTGCCGATCAGACGTTGAGTGAATCCGTGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCgag < 1:1539636/100‑1 (MQ=255) aaTGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCgag > 1:1162201/1‑100 (MQ=255) tGCCGATCAGACGTTGAGTGAAACCTGGAGCTGCCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGaa > 2:1471161/1‑100 (MQ=255) ccGACCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTTACGCAATGCCCATCGCCGCAAGCGAGAAAg > 2:204582/1‑100 (MQ=255) cGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAg < 2:1766478/99‑1 (MQ=255) cGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGc > 1:1453100/1‑100 (MQ=255) gATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCg < 1:678984/100‑1 (MQ=255) tCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGc > 1:1806166/1‑100 (MQ=255) cAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCa > 2:1396331/1‑100 (MQ=255) aGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCAt > 1:774940/1‑100 (MQ=255) gACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCAtt < 2:1117151/100‑1 (MQ=255) gTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGcc < 1:1817130/100‑1 (MQ=255) aGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAg > 1:1082414/1‑100 (MQ=255) aGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAg < 1:1508/100‑1 (MQ=255) aaaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTg < 1:472104/100‑1 (MQ=255) aaaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTg > 2:1040505/1‑100 (MQ=255) aaaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCAGCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTg < 1:1402567/100‑1 (MQ=255) aaCGTGGATCTACCAGTTACTTGCCAAAGAATAAGGCAACTGAGTACATTGACGCAATGCCTACCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGa < 2:594351/100‑1 (MQ=255) aaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGa < 1:1407873/100‑1 (MQ=255) ccTGGAGCTACTAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGata > 2:1514983/1‑100 (MQ=255) cTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCgcgc > 2:169844/1‑100 (MQ=255) cTACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCgcgc < 2:66383/100‑1 (MQ=255) cTACCAGTTACCTGCCAATGAATAAGACAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCgcgc > 1:1474415/1‑100 (MQ=255) tACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGcc > 1:347061/1‑100 (MQ=255) tACCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGcc > 1:847056/1‑100 (MQ=255) aCCAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCg > 1:424405/1‑100 (MQ=255) ccAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGt < 1:668896/100‑1 (MQ=255) cAGTTACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGtt > 2:1412583/1‑100 (MQ=255) ttACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATGGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTtca < 1:608505/99‑1 (MQ=255) tACCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTtcatc > 2:12860/1‑100 (MQ=255) aCCTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTtcatca > 2:1559240/1‑100 (MQ=255) cTGCCAATGAATAAGGCAACTGAGTACATTGACTCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAgg > 1:1199564/1‑100 (MQ=255) cTGCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAgg > 1:1710273/1‑100 (MQ=255) gCCAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGgcg > 1:948852/1‑100 (MQ=255) ccAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGgcgc < 2:847062/100‑1 (MQ=255) ccAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGgcgc > 2:476636/1‑100 (MQ=255) ccAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGgcgc > 2:1296528/1‑100 (MQ=255) cAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCt > 1:911269/1‑100 (MQ=255) cAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCt > 1:1042117/1‑100 (MQ=255) cAATGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCt < 1:1040495/100‑1 (MQ=255) tGAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGa < 2:543107/100‑1 (MQ=255) gAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGAt > 1:960711/1‑100 (MQ=255) gAATAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGAt > 2:607908/1‑100 (MQ=255) aaTAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATg > 1:1592761/1‑100 (MQ=255) tAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTTCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGCATGcc > 1:1449926/1‑100 (MQ=255) tAAGGCAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGcc < 1:658485/100‑1 (MQ=255) | GATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGACAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCC > NC_000913/1110775‑1110967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |