| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,143,641 | G→A | H243Y (CAT→TAT) | rne ← | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,143,641 | 0 | G | A | 98.8% | 277.4 / ‑4.3 | 81 | H243Y (CAT→TAT) | rne | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (42/38); total (43/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCT > NC_000913/1143544‑1143737 | aTCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACt < 1:229004/100‑1 (MQ=255) aCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg > 2:1133493/1‑99 (MQ=255) tctaTCTGGTAGCGGCTGACCAGCGGGAGCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc < 1:224550/96‑1 (MQ=255) cGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGCGCGACCTAATGCAGCGATATACTGACGTGCCAg < 1:1093890/100‑1 (MQ=255) aTCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGtt > 1:1481429/1‑100 (MQ=255) cTGGTAGTGGCTTAACAGCGGGATCTCGCCTGGGTAAAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGACAGtt < 1:520130/98‑1 (MQ=255) cTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTc > 1:1141494/1‑99 (MQ=255) ggTAGTGGCTGAACAGCGGGATCTGGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg < 2:1190266/100‑1 (MQ=255) ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGa > 1:1572822/1‑99 (MQ=255) ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg < 1:1202728/100‑1 (MQ=255) ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg < 1:1087259/100‑1 (MQ=255) ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg < 2:1410944/100‑1 (MQ=255) gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc < 2:1543874/100‑1 (MQ=255) gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc < 2:479963/100‑1 (MQ=255) gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc > 2:1794923/1‑100 (MQ=255) tAGTGGCTGAACAGCGGGATCTCGTCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg < 2:392321/98‑1 (MQ=255) aGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCAc > 1:337059/1‑100 (MQ=255) ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt < 1:1221333/100‑1 (MQ=255) cTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTACTGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCg > 1:1166375/1‑100 (MQ=255) gAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg < 2:714856/100‑1 (MQ=255) aaCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGt > 2:1420597/1‑100 (MQ=255) aCAGCGGGAATTCGCCGGTGTACAGTTTGTTTTTGCTGCTGAAAGCCGGGCGACGTAATACAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGt < 1:478895/99‑1 (MQ=255) cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGt < 1:1603308/98‑1 (MQ=255) aGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTAt < 1:1115619/100‑1 (MQ=255) gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc < 2:960567/100‑1 (MQ=255) gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc < 1:979531/100‑1 (MQ=255) gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc > 2:1579800/1‑100 (MQ=255) gCGGGATCTCGCCGGCGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc < 2:1793430/100‑1 (MQ=255) cGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCg < 2:1077575/100‑1 (MQ=255) gtaTCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATAAACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCAAt > 2:1684361/3‑100 (MQ=255) gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa < 2:1141505/100‑1 (MQ=255) gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa < 1:31415/100‑1 (MQ=255) aTCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATaa < 1:1265619/100‑1 (MQ=255) tctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAg < 1:1667577/100‑1 (MQ=255) ctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCATTTCGAGCACTTTCGGGTTATCGATaa > 1:57050/1‑98 (MQ=255) ctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAgg > 2:119475/1‑100 (MQ=255) ctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAgg > 2:705374/1‑100 (MQ=255) tcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGa > 1:94230/1‑100 (MQ=255) cGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTc < 1:1796428/100‑1 (MQ=255) ggTGTACAGCTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCg > 1:1701597/1‑100 (MQ=255) gtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCg < 2:67488/100‑1 (MQ=255) tACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa < 2:1392456/100‑1 (MQ=255) aGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg < 1:1077563/99‑1 (MQ=255) gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc > 1:53406/1‑100 (MQ=255) gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc > 1:1292464/1‑100 (MQ=255) gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc > 2:1127745/1‑100 (MQ=255) gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCAGGTTATCGATAAGGATTTCGCCGATGTc > 2:92658/1‑100 (MQ=255) tGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCAGATGTCCTg > 2:170972/1‑100 (MQ=255) gATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGa < 1:284957/100‑1 (MQ=255) aTTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc > 1:1115810/1‑100 (MQ=255) ttttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc > 1:845462/1‑99 (MQ=255) gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag < 2:352771/100‑1 (MQ=255) ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGATATCGATAAGGATTTCGCCGATGTCCTGACgtaagt > 2:97547/1‑100 (MQ=255) gctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagtaa > 1:107564/1‑100 (MQ=255) gctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagtaa > 1:582049/1‑100 (MQ=255) gctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta > 1:1433406/1‑99 (MQ=255) ctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAAt > 1:876861/1‑100 (MQ=255) ctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAAt > 1:1290879/1‑100 (MQ=255) ctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCAAATGTCCTGAAgtaagaaa > 2:666344/1‑99 (MQ=255) tgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTCCGCCGATGTCCTGACGTAAGTAATc > 1:927438/1‑100 (MQ=255) gAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcg > 1:249605/1‑100 (MQ=255) naTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGGACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgcg > 1:1007560/2‑100 (MQ=255) aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg > 2:593247/1‑100 (MQ=255) cGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAAt > 1:596938/1‑100 (MQ=255) ggCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAAt > 1:1169529/1‑98 (MQ=255) cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTACTGACGTAAATAATCGCGGaa > 2:116627/1‑95 (MQ=255) ccTAATGCAGCGATATACTGGCGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGaa > 1:1107189/1‑100 (MQ=255) cTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAAc < 1:1420586/100‑1 (MQ=255) cTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAAc < 2:61959/100‑1 (MQ=255) tAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg < 1:1746992/100‑1 (MQ=255) tAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg < 2:1290890/100‑1 (MQ=255) tAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg > 2:1136627/1‑100 (MQ=255) aaTGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGa > 1:1561748/1‑100 (MQ=255) tGCAGCGATATATTGACGTACCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGCTGTCCTGACGTAAGTAATCGCGGACTGCGCGAACGAtt > 1:695232/1‑100 (MQ=255) gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa > 2:245310/1‑100 (MQ=255) gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa > 1:27532/1‑100 (MQ=255) gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa < 1:119475/100‑1 (MQ=255) cGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGtt > 1:392042/1‑100 (MQ=255) atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc < 1:1579789/100‑1 (MQ=255) atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc < 2:1701610/100‑1 (MQ=255) atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc < 2:1445446/100‑1 (MQ=255) tataCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGct < 2:1292475/100‑1 (MQ=255) | ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCT > NC_000913/1143544‑1143737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |