Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,184,649 A→G Y316H (TAC→CAC)  potA ← spermidine/putrescine ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,184,6490AG96.2% 253.9 / ‑3.1 80Y316H (TAC→CAC) potAspermidine/putrescine ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (48/28);  total (51/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTCATTGAAGAATTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTAACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCGGTTC  >  NC_000913/1184552‑1184742
                                                                                                 |                                                                                             
tCTTCATTGAAGAATTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGAc                                                                                             <  2:220855/100‑1 (MQ=255)
 cTTCATTGAAGAATTCACTGACAATCACCATCTTGCCATTTTCCACTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCTCGAACGTCAcc                                                                                            >  1:1405751/1‑100 (MQ=255)
   tCATTGAAGAATTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTTCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCaa                                                                                          <  1:993135/100‑1 (MQ=255)
   tCATTGAAGAATTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTAACaaa                                                                                          >  2:377515/1‑100 (MQ=255)
     aTTGAAGAATTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATc                                                                                        >  1:1376666/1‑100 (MQ=255)
      ttGAAGAATTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAAAGTGACCAATCa                                                                                       >  2:359400/1‑100 (MQ=255)
       tGAAGAATTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAg                                                                                      <  1:34176/100‑1 (MQ=255)
        gaagaaTTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGc                                                                                     >  2:1563961/1‑100 (MQ=255)
        gaagaaTTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGc                                                                                     >  2:829330/1‑100 (MQ=255)
        gaagaaTTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCACGAACGTGACCAATCAGc                                                                                     >  2:664516/1‑100 (MQ=255)
         aacaaTTCGCTGACCATCACCATCTTGCCACTGTCCTGTTCATCACCCGAACCCAGCGTCATGCCTTTTTATTTACTCTTGCGAACGTGACCTATCAGcc                                                                                    <  1:896186/97‑1 (MQ=255)
          agaaTTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGccc                                                                                   >  1:1741627/1‑100 (MQ=255)
               tCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAg                                                                              <  1:757175/100‑1 (MQ=255)
                cGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGc                                                                             >  1:1296289/1‑100 (MQ=255)
                   tGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTg                                                                          <  2:1239404/100‑1 (MQ=255)
                      ccatcaccatcTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGtt                                                                       >  1:1298982/1‑100 (MQ=255)
                      caatcaccatcTTGCCATTTTCAAGTTCAACAACCGACTCAAGCGTCATGCCTTTGTAGTTACGCCTGCGAACGTGACAAATCAGCCCTTCAGCGTgat                                                                        >  1:1381508/3‑97 (MQ=255)
                       catcaccatcTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTg                                                                      <  2:610490/100‑1 (MQ=255)
                         tcaccatcTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACACTCGCGAACGTGACCAATCAACCCTTCAGCGTGGTTgt                                                                     >  2:661319/1‑99 (MQ=255)
                             catcTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGtcgtcg                                                                <  2:592441/100‑1 (MQ=255)
                              atcTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTcgtcgt                                                               <  1:1278254/100‑1 (MQ=255)
                                 ttGCCATTTTCCAGTTCAACACCCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGCCGTCGTTaa                                                            >  1:1439739/1‑100 (MQ=255)
                                  tGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAAt                                                           <  1:1561527/100‑1 (MQ=255)
                                   gCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAAtc                                                          >  2:1713945/1‑100 (MQ=255)
                                    ccATTTTCCAGTTCAACAAACGACTCCAGCGTCATGCCTTTGTAGTTACCCTCCCGCACGTGACCAATCAGCCATTCAGAGCGGTTCTAGTCGTTaa                                                            >  1:1269994/1‑97 (MQ=255)
                                      aTTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAAtc                                                          <  2:859978/97‑1 (MQ=255)
                                      aTTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATctct                                                       <  2:1745616/100‑1 (MQ=255)
                                      aTTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATctct                                                       >  1:44575/1‑100 (MQ=255)
                                        tttCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAATGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTc                                                     <  1:1707536/100‑1 (MQ=255)
                                        tttCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCtta                                                     <  1:640081/100‑2 (MQ=255)
                                        tttCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTc                                                     <  2:1187375/100‑1 (MQ=255)
                                         ttCCAGTTCAACAACCGTCTCCAGCGTCATGCCTTTGTAGTTACGTTCGCGAACGTGACCAATCGGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCa                                                    <  1:371834/100‑1 (MQ=255)
                                          tCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCaa                                                   >  1:127294/1‑100 (MQ=255)
                                          tCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCaa                                                   >  1:1043684/1‑100 (MQ=255)
                                            cAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAaca                                                 >  1:368488/1‑100 (MQ=255)
                                            cAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAaca                                                 >  1:328267/1‑100 (MQ=255)
                                            cAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAaca                                                 >  2:1452309/1‑100 (MQ=255)
                                            cAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAac                                                  <  2:261713/99‑1 (MQ=255)
                                             aGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAacac                                                >  2:1359841/1‑100 (MQ=255)
                                              gTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAacac                                                >  1:1393972/1‑99 (MQ=255)
                                               ttCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGt                                              >  2:1555642/1‑100 (MQ=255)
                                               ttCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGt                                              >  2:1089837/1‑100 (MQ=255)
                                               ttCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGt                                              >  1:416418/1‑100 (MQ=255)
                                                 caacaaCCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTaa                                            >  2:807921/1‑100 (MQ=255)
                                                  aacaacCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTAACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAg                                           <  1:550846/100‑1 (MQ=255)
                                                   acaacCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGt                                          <  2:818305/100‑1 (MQ=255)
                                                    caacCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTc                                         >  2:258142/1‑100 (MQ=255)
                                                    caacCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAATc                                         >  2:258404/1‑100 (MQ=255)
                                                      acCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTAGTAGTCGTTAATCTCTTCAACACGTAAGTCtt                                       >  2:110330/1‑100 (MQ=255)
                                                       ccGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTc                                      >  2:618706/1‑100 (MQ=255)
                                                         gACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCg                                    <  2:182730/100‑1 (MQ=255)
                                                         gACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCg                                    >  2:1006726/1‑100 (MQ=255)
                                                           cTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCggg                                  >  1:1260938/1‑100 (MQ=255)
                                                              cAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGgcgc                               >  1:280859/1‑100 (MQ=255)
                                                              cAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTACGGgcgc                               >  2:1557412/1‑100 (MQ=255)
                                                              cAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTAGATAATCCCTTCAACACGTAAGCCTTCAGGgc                                 >  1:529215/1‑98 (MQ=255)
                                                                 cGTCATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCgcagc                            <  1:829830/100‑1 (MQ=255)
                                                                    cATGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATATCTTCAACACGTAAGTCTTCCGGGCGCAGCAGa                         >  1:431609/1‑100 (MQ=255)
                                                                      tGCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAAc                       <  2:1727666/100‑1 (MQ=255)
                                                                       gCCTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACa                      >  2:1241338/1‑100 (MQ=255)
                                                                        ccTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACAt                     >  2:1311077/1‑100 (MQ=255)
                                                                        ccTTTGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACAt                     <  2:1376676/100‑1 (MQ=255)
                                                                        ccTTTGTAGTTACGCTCGCGAACGTAACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACAt                     >  1:1290337/1‑100 (MQ=255)
                                                                          tttGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGc                   >  1:1696981/1‑100 (MQ=255)
                                                                           ttGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCa                  >  1:314297/1‑100 (MQ=255)
                                                                            tGTAGTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAg                 <  2:525951/100‑1 (MQ=255)
                                                                                gTTACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGtttt             >  2:1239193/1‑100 (MQ=255)
                                                                                  tACGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTg           <  1:998978/100‑1 (MQ=255)
                                                                                    cGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGcc         >  1:1229666/1‑100 (MQ=255)
                                                                                    cGCTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGcc         <  2:127294/100‑1 (MQ=255)
                                                                                      cTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGcccc       >  2:62301/1‑100 (MQ=255)
                                                                                      cTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGcccc       >  1:1291256/1‑100 (MQ=255)
                                                                                      cTCGCGAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGcccc       >  1:698616/1‑100 (MQ=255)
                                                                                        cgcgAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCgg     <  1:377513/100‑1 (MQ=255)
                                                                                        cgcgAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCgg     >  2:1802604/1‑100 (MQ=255)
                                                                                        cgcgAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCgg     >  2:1759776/1‑100 (MQ=255)
                                                                                        cgcgAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCgg     <  2:1361320/100‑1 (MQ=255)
                                                                                        cgcgAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCgg     >  2:1199679/1‑100 (MQ=255)
                                                                                          cgAACGTGACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCGGtt   >  1:255643/1‑100 (MQ=255)
                                                                                           gAACGTGACCAATCAGCCCTTCTGCGTGGTTGTCGTCGTTAATCTCTTCAACACGGAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCGGTTc  <  2:709493/100‑1 (MQ=255)
                                                                                                 |                                                                                             
TCTTCATTGAAGAATTCGCTGACCATCACCATCTTGCCATTTTCCAGTTCAACAACCGACTCCAGCGTCATGCCTTTGTAGTTACGCTCGCGAACGTAACCAATCAGCCCTTCAGCGTGGTTGTCGTCGTTAATCTCTTCAACACGTAAGTCTTCCGGGCGCAGCAGAACATGCAGTTTTTGCCCCGGTTC  >  NC_000913/1184552‑1184742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑