Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,698,281	A→G	V300V (GTT→GTC)	malI ←	transcriptional repressor of Mal regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,698,281	0	A	G	100.0%	272.5 / NA	76	V300V (GTT→GTC)	malI	transcriptional repressor of Mal regulon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/38);  total (38/38)

AGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTT  >  NC_000913/1698186‑1698377                                                                                                |                                                                                                 aGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAg                                                                                              <  2:380496/100‑1 (MQ=255) aGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAg                                                                                              >  2:1527404/1‑100 (MQ=255)  gATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAgg                                                                                             >  2:1117191/1‑100 (MQ=255)  gATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAgg                                                                                             <  1:717573/100‑1 (MQ=255)  gATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAgg                                                                                             <  1:1628061/100‑1 (MQ=255)      gCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGtata                                                                                         <  1:838868/100‑1 (MQ=255)       cGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTatat                                                                                        >  1:1507183/1‑100 (MQ=255)         tGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATatca                                                                                      <  1:371335/100‑1 (MQ=255)           aaTGCGTCTCTTCATGTGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATatcatc                                                                                    <  2:549753/100‑1 (MQ=255)           aaTGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATatcatc                                                                                    <  1:593666/100‑1 (MQ=255)            aTGCGTCTCTTCATGGGTGATTTTTTGCATCGTGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATAtcatca                                                                                   <  1:1221579/100‑1 (MQ=255)             tgtttctcTTCATGGGTGATTTTTTGCATCATGCTATCTGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATAtcatca                                                                                   <  2:562082/95‑1 (MQ=255)              gCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATTCGCAAGTGTGATACAAAGTTCCGGCGCTGGCGTGCTGGCCTAGGTGACAGGTATATCATCAAg                                                                                 >  1:1308104/1‑100 (MQ=255)               cGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAgt                                                                                <  1:1338102/100‑1 (MQ=255)               cGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAgt                                                                                <  1:1339549/100‑1 (MQ=255)               cGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAgt                                                                                >  2:1442146/1‑100 (MQ=255)                gTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAgtg                                                                               <  1:707095/100‑1 (MQ=255)                  ctctTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGtgtg                                                                             <  1:139887/100‑1 (MQ=255)                   tctTCATGGGTGATTTTTTGCATCATGCGATTCGCAAGTGTTATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGtgg                                                                            <  2:84500/100‑1 (MQ=255)                    ctTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGtggt                                                                           <  1:1782690/100‑1 (MQ=255)                         tctgTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGtggttgg                                                                        <  2:1308054/95‑1 (MQ=255)                         tGGGTGATTTTTTGCATCATGCGCTCCGCAAGTGTGATCCCAAGTTCCCGCGCTAGCGTGCTGGCCCAGGTGACGTGTATATCATCAAGTGtggttgg                                                                        <  1:1243403/98‑1 (MQ=255)                         tGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGgtg                                                                      >  1:1210835/1‑100 (MQ=255)                          gggTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGgtgt                                                                     >  2:1519821/1‑100 (MQ=255)                            gTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGtggttggagatc                                                                   >  1:1308044/1‑95 (MQ=255)                            gTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCg                                                                   >  2:1579362/1‑100 (MQ=255)                            gTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCg                                                                   >  1:1334467/1‑100 (MQ=255)                            gTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCg                                                                   <  2:63033/100‑1 (MQ=255)                                ttttttGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATc                                                               <  1:1616651/100‑1 (MQ=255)                                 tttttGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCg                                                              >  2:1578110/1‑100 (MQ=255)                                 tttttGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCg                                                              >  1:774678/1‑100 (MQ=255)                                  ttttGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCgg                                                             <  2:1531771/100‑1 (MQ=255)                                  ttttGCATCATGCGATCCGCAAGTGGGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCg                                                              <  1:981072/99‑1 (MQ=255)                                   tttGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGt                                                            <  2:840607/100‑1 (MQ=255)                                     tGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTaa                                                          >  1:621376/1‑100 (MQ=255)                                        atcatGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATg                                                       >  2:733356/1‑100 (MQ=255)                                        atcatGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATg                                                       >  2:1423211/1‑100 (MQ=255)                                        atcatGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATg                                                       <  2:1321055/100‑1 (MQ=255)                                             gCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATgccgcc                                                  <  1:22850/100‑1 (MQ=255)                                             gCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATgccgcc                                                  >  1:851532/1‑100 (MQ=255)                                              cGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCa                                                 >  2:1041204/1‑100 (MQ=255)                                                  ccGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGa                                             >  1:274096/1‑100 (MQ=255)                                                   cGCAAGTGTGATACCAAGTTCCCGCGCTGGAGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGaa                                            <  1:1703126/100‑1 (MQ=255)                                                    gCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGaaa                                           <  2:259362/100‑1 (MQ=255)                                                     cAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGacac                                          >  2:1290725/1‑100 (MQ=255)                                                          gtgATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGtt                                     >  1:484919/1‑100 (MQ=255)                                                          gtgATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGACGCCAGCGAAACCtttt                                     >  2:1690239/1‑100 (MQ=255)                                                            taTACCAAGTTCCCGCGCTGGCGTGGTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGc                                   <  2:1194514/99‑1 (MQ=255)                                                            gATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGc                                   <  1:1041194/100‑1 (MQ=255)                                                             aTACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGc                                   <  1:1622554/99‑1 (MQ=255)                                                             aTACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCAGCCAGCGAAACCTGTTGCt                                  >  2:722568/1‑100 (MQ=255)                                                              tACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTc                                 >  2:1006799/1‑100 (MQ=255)                                                               aCCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTTTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCa                                <  1:1117181/100‑1 (MQ=255)                                                               aCCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCa                                <  2:1534309/100‑1 (MQ=255)                                                                ccAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTTTGGTTAGTGACGCATCAGTAAATGCCGCCAGCGAAACCAGTTGCTCa                                >  2:386678/1‑99 (MQ=255)                                                                 cAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCaa                               <  1:1579351/99‑1 (MQ=255)                                                                  aaGTTCCCGCGCTGGCGTGCTGGCCCAGGTGAGAGGTATATCATCAAGTGTGGTTGGTGGCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAg                             >  2:1726212/1‑100 (MQ=255)                                                                  aaGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAg                             <  1:1527393/100‑1 (MQ=255)                                                                  aaGTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAg                             <  2:851538/100‑1 (MQ=255)                                                                    gTTCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTa                           >  2:1573581/1‑100 (MQ=255)                                                                      tCCCGCGCTGGCGTGCTGGCCCAGGTGACAGGTATACCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAAc                         <  2:1273178/100‑1 (MQ=255)                                                                       cccGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACg                        >  2:430862/1‑100 (MQ=255)                                                                        ccGCGCTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGa                       >  1:673204/1‑100 (MQ=255)                                                                           cgcTGGCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCg                    >  2:113794/1‑100 (MQ=255)                                                                                gCGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTcc               <  2:898689/100‑1 (MQ=255)                                                                                 cGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCg              >  2:964171/1‑100 (MQ=255)                                                                                 cGTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCg              >  1:1334404/1‑100 (MQ=255)                                                                                  gTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGc             >  2:1684795/1‑100 (MQ=255)                                                                                  gTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGc             <  1:381045/100‑1 (MQ=255)                                                                                  gTGCTGGCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGc             <  1:1519810/100‑1 (MQ=255)                                                                                       ggCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTcc        >  2:1601141/1‑100 (MQ=255)                                                                                       ggCCCAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTcc        >  2:1248811/1‑100 (MQ=255)                                                                                         cccAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTcccc      >  1:707089/1‑100 (MQ=255)                                                                                           cAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGc    >  2:848685/1‑100 (MQ=255)                                                                                           cAGGTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGc    >  1:1478968/1‑100 (MQ=255)                                                                                             ggTGACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCtt  >  1:1087937/1‑100 (MQ=255)                                                                                                |                                                                                                 AGATTGCGTGAATGCGTCTCTTCATGGGTGATTTTTTGCATCATGCGATCCGCAAGTGTGATACCAAGTTCCCGCGCTGGCGTGCTGGCCCAGGTAACAGGTATATCATCAAGTGTGGTTGGTGTCGCATCGGTAAATGCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTT  >  NC_000913/1698186‑1698377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑